[논문]오수유 물 추출물의 선천 면역 활성과 염증 억제 효과

정소미; 이진무; 이창훈; 황덕상; 장준복

doi:10.15204/jkobgy.2021.34.2.001

오수유 물 추출물의 선천 면역 활성과 염증 억제 효과
Innate Immunity Activation and Anti-Inflammation Effects of Evodia Rutaecarpine Water Extract 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.2, 2021년, pp.1 - 15

정소미 (경희대학교 대학원 임상한의학과) , 이진무 (경희대학교 대학원 임상한의학과) , 이창훈 (경희대학교 대학원 임상한의학과) , 황덕상 (경희대학교 대학원 임상한의학과) , 장준복 (경희대학교 대학원 임상한의학과)

Abstract ▼ AI-Helper

Objectives: This study was designed to examine immuno-modulatory effects of Evodia Rutaecarpine by activating innate immune system and inhibiting inflammation. Methods: First, Cell cytotoxicity was examined with 4T1 breast carcinoma and TG-induced macrophage. To investigate activating innate immune system of Evodiamine Rutacarpine Extract (ERE) on macrophage, we tested tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with ERE to observe innate immune modulating effect of ERE on RAW 264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In cytotoxicity analysis, ERE significantly affected tumor cell growth above specific concentration. Also, ERE significantly affected macrophage growth above specific concetration. As compared with the control group, the production of TNF-α, IL-12 and IL-6 were increased in TG-induced macrophage. As compared with the control group, TNF-α and IL-6 were significantly up-regulated in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating ERE was significantly decreased compared with control group. In addition, We observed ERE inhibited the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38 in western blotting by treating ERE on RAW 264.7 cell. Conclusions: ERE seems to have considerable impact on the anti-cancer effect by activation of innate immune system and inflammation control.

주제어

표/그림 (11)

그림 Fig. 1. Cytotoxicity of ERE on 4T1 breast carcinoma cell. **: p<0.01, statistically significant difference compared with media group
그림 Fig. 2. Cytotoxicity of ERE on RAW 264.7 cell. *: p<0.05, statistically significant difference compared with media group
그림 Fig 3. Production of TNF-α from TGinduced macrophages stimulated by ERE. *: p<0.05, statistically significant difference compared with media group
그림 Fig. 4. Production of IL-12 from TG-induced macrophages stimulated by ERE. *: p<0.05, statistically significant difference compared with media group
그림 Fig. 5. Production of IL-6 from TG-induced macrophage stimulated by ERE. *: p<0.05, statistically significant difference compared with media group
그림 Fig. 6. Production of TNF-α from RAW 264.7 cell stimulated by ERE. **: p<0.01, statistically significant difference compared with media group
그림 Fig. 7. Production of IL-6 from RAW 264.7cell stimulated by ERE. *: p<0.05, statistically significant difference compared with media group
그림 Fig. 8. Production of TNF-α from RAW 264.7 cell before and after stimulation of LPS. *: p<0.05, **: p<0.01, statistically significant difference compared with LPS treated group
그림 Fig. 9. Production of NO from RAW 264.7 cell before and after stimulation of LPS. **: p<0.01, statistically significant difference compared with LPS treated group
그림 Fig. 10. Inhibition of NF-κB pathway by treatment of ERE and LPS on RAW 264.7 cell.
그림 Fig. 11. Inhibition of MAPK pathway by treatment of ERE and LPS on RAW 264.7 cell.

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