[논문]고당 및 올레산으로 유도된 간세포에서의 염증반응에 대한 말차(Camellia sinensis) 추출물의 보호효과

김종민; 이욱; 강진용; 박선경; 신은진; 문종현; 김민지; 이효림; 김길한; 정혜린; 박효원; 김종철; 허호진

doi:10.9721/kjfst.2021.53.3.267

고당 및 올레산으로 유도된 간세포에서의 염증반응에 대한 말차(Camellia sinensis) 추출물의 보호효과
Protective effect of matcha green tea (Camellia sinensis) extract on high glucose- and oleic acid-induced hepatic inflammatory effect 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.3, 2021년, pp.267 - 277

김종민 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 이욱 (국립산림과학원 산림특용자원연구과) , 강진용 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 박선경 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 신은진 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 문종현 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 김민지 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 이효림 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 김길한 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 정혜린 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) , 박효원 (국립산림과학원 산림특용자원연구과) , 김종철 ((재)하동녹차연구소) , 허호진 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원)

초록
AI-Helper

본 연구는 가루녹차의 가공품인 말차를 이용하여 항산화 평가와 고당으로 유도된 간세포에서의 간세포 보호효과와 지방간 개선 활성을 평가하기 위해 진행되었다. 말차의 catechin 함량과 총 페놀성 화합물 및 총 플라보노이드 화합물의 함량은 잎 녹차보다 우수한 함량을 나타내었으며, 라디칼 소거활성과 지질과산화물 억제활성 역시 잎 녹차보다 우수한 것을 확인하였다. 또한, 항당뇨 활성을 나타내는 α-glucosidase, α-amylase 및 최종당화산물에 대한 우수한 억제활성을 확인하였다. In vitro 간세포 보호효과를 평가한 결과, 말차는 효과적으로 산화적 스트레스 및 고당으로 인한 활성산소 생성 억제활성과 간세포 생존율 나타내었다. 또한, oleic acid로 유도된 지방 축적에 대한 말차 추출물의 억제활성을 확인하였으며, 지방간으로 인한 염증반응에 대한 조절을 확인하였다. 이러한 연구 결과를 바탕으로 녹차의 가공품 중 하나인 말차는 가공 전 잎 녹차에 비해 우수한 카테킨 함량과 항산화 활성을 지니며, 탄수화물의 섭취를 억제해주는 소화효소의 활성을 억제하여 간세포의 지질축적을 억제하는 데 도움을 줄 수 있을 뿐만 아니라 염증 감소에 도움을 줄 수 있는 소재로서의 가능성을 확인하였다.

Abstract ▼ AI-Helper

To evaluate hepatoprotective effects, the antioxidant capacities of matcha green tea extract (Camellia sinenesis) were compared to those of green leaf tea and the anti-inflammatory activities in HepG2 cells were investigated. Evaluation of the total phenolic and total flavonoid content, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and inhibitory effect on lipid peroxidation indicated that the aqueous extract of matcha green tea presented significant catechin content and antioxidant capacity compared to those of green leaf tea. In addition, the extract had considerable inhibitory effects on α-glucosidase, α-amylase, and advanced glycation end-products. The matcha green tea extract significantly increased cell viability and reduced reactive oxygen species in H2O2- and high-glucose-treated HepG2 cells. Furthermore, in response to oleic acid-induced HepG2 cell injury, treatment with matcha green tea aqueous extract inhibited lipid accumulation and regulated the expression of inflammatory proteins such as p-JNK, p-Akt, p-GSK-3β, caspase-3, COX-2, iNOS, and TNF-α. Matcha green tea could be used as a functional material to ameliorate hepatic lipid accumulation and inflammation.

주제어

표/그림 (7)

표 Table 1. Catechin conetents of aqueous extract of leaf green tea and matcha green tea (Unit: mg/g extract)
표 Table 2. Antioxidant capacity of aqueous extract of leaf green tea and matcha green tea
그림 Fig. 1. α-Amylase inhibitory effect (A), α-glucosidase inhibitory effect (B) and advanced glycation end products (AGEs) formation inhibitory activity (C) of aqueous extract of matcha. Results shown are mean±SD (n=3). Data were statistically represented at p<0.05, and different small letters represent statistical differences.
그림 Fig. 2. Reactive oxygen species (ROS) inhibitory effect of aqueous extract of matcha on H₂O₂-induced (A) and high glucose-induced (B) cellular oxidative stress and hepatic cell viability of aqueous extract of matcha on H₂O₂-induced (C) and high glucose-induced (D) cytotoxicity in HepG2 cells. Results shown are means±SD (n=5). Data were statistically represented at p<0.05, and different small letters represent statistical differences.
그림 Fig. 3. Inhibitory effect of aqueous extract of matcha on oleic acid-induced cell viability (A) and lipid accumulation (B) in HepG2 cells. Results shown are means±SD (n=5). Data were statistically represented at p<0.05, and different small letters represent statistical differences.
그림 Fig. 4. Protective effect of aqueous extract of matcha on oleic acid-induced inflammatory pathway in HepG2 cells. (A) Representative western blots for total protein and expression of p-JNK, p-Akt, p-GSK-3β, caspase-3, COX-2, iNOS, TNF-α and β-actin; Protein expression levels of p-JNK (B); p-Akt (C); p-GSK-3β (D); caspase-3 (E); COX-2 (F), iNOS (G) and TNF-α (H) normalized to β-actin. Results shown are mean±SD (n=3). Data were statistically represented at p<0.05, and different small letters represent statistical differences.
그림 Fig. 4. Continued

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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