[논문]채취 시기 및 추출 용매에 따른 환삼덩굴의 항산화 및 기능성 화장품 활성

채정우; 조희선; 염현지; 이진영

doi:10.14578/jkfs.2021.110.2.254

[국내논문] 채취 시기 및 추출 용매에 따른 환삼덩굴의 항산화 및 기능성 화장품 활성
Antioxidation and Functional Cosmetics Activity of Humulus japonicus Sieboid & Zucc. According to Collection Time and Extraction Solvent 원문보기

한국산림과학회지 = Journal of korean society of forest science, v.110 no.2, 2021년, pp.254 - 265

채정우 (경기도산림환경연구소) , 조희선 (경기도산림환경연구소) , 염현지 (호서대학교 화장품생명공학부) , 이진영 (호서대학교 화장품생명공학부)

초록
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채취 시기 및 추출 용매에 따른 환삼덩굴 추출물의 총 폴리페놀 함량을 측정한 결과 6월에 채취한 환삼덩굴의 열수 추출물의 총 폴리페놀 함량은 41.09 mg/g GAE, 같은 시기에 채취한 70% 에탄올 추출물은 56.64 mg/g GAE를 나타내었으며, 8월에 채취하여 추출한 열수 추출물은 31.56 mg/g GAE, 에탄올 추출물은 31.81 mg/g GAE의 함량을 나타내어, 열수와 에탄올 추출물 모두 6월에 채취하여 추출한 것이 총 폴리페놀 함량이 높게 나타났으며, 채취 시기가 같은 경우 열수 추출물보다 70% 에탄올 추출물의 총 폴리페놀 함량이 높은 것으로 나타났다. 전자공여능, SOD 유사활성, ABTS radical 소거능 측정을 통하여 환삼덩굴 추출물의 항산화 활성을 측정한 결과 모든 실험에서 총 폴리페놀 함량이 가장 높게 나타난 6월 채취 70% 에탄올 추출물의 활성이 가장 높았다. 또한 8월 채취 추출물의 경우 총 폴리페놀 함량은 비슷하였으나 모든 실험에서 에탄올 추출물의 활성이 높게 나타나 항산화에 관여하는 물질은 70% 에탄올로 추출하였을 때 활성이 높게 유지된다고 판단되었다. 미백활성 평가를 위한 tyrosinase 저해활성 측정 결과 환삼덩굴 열수 추출물은 6월 채취 추출물은 활성을 나타내지 않았으며 8월 채취 추출물은 최고농도에서의 활성이 16.18%로 나타났다. 에탄올 추출물의 경우 최고농도의 활성이 6월 채취 추출물은 8.07%, 8월 추출물이 14.7%로 나타나 미백활성을 나타내는 성분은 6월보다는 8월에 함량이 높은 것으로 판단되었다. 피부 주름 생성 방지 활성 평가를 위한 elastase 저해활성의 경우 에탄올 추출물은 매우 미미한 활성이 나타났으며 열수 추출물은 활성을 나타내지 않았다. 또한 모든 추출물에서 수렴활성이 나타나지 않아 환삼덩굴을 주름 생성 방지를 위한 기능성 원료로 사용하는 것은 적절하지 않다고 판단된다. 총 폴리페놀 함량과 항산화 활성이 가장 높게 나타난 6월 채취 환삼덩굴 에탄올 추출물의 세포독성(MTT assay) 결과, 저농도인 5~10 ㎍/mL에서는 세포증식효과를 나타내었으나 50 ㎍/mL에서 64.13%의 세포 생존율을 보여 강한 세포독성을 나타내었다. NO 생성 억제 능력의 경우, 농도가 증가함에 따라 Raw 264.7의 NO 생성이 억제되어 1,000 ㎍/mL에서의 NO 생성량이 40.7%까지 감소하였다. 그러나 이러한 결과가 세포 독성에 의한 것인지, 추출물의 효능에 의한 것인지는 추가 연구가 필요한 부분이다.

Abstract ▼ AI-Helper

As a result of measuring the polyphenol content of Humulus japonicus (HJ) extract according to the collection time and extraction solvent, bothhot water extract and 70% ethanol extract were collected and extracted in June, and the polyphenol content was high. When the harvesting time was the same, the polyphenol content of the ethanol extract was higher than that of the hot water extract. As a result of measuring the antioxidant activity of HJ extract by measuring electron-donating ability, SOD-like activity, and ABTS radical scavenging ability, HJ6E, which has the highest polyphenol content, showed the highest activity. In addition, in the case of the extract collected in August, the polyphenol content was similar. However, the antioxidant activity of the ethanol extract was high, sothe antioxidant activity remained high when extracted with 70% ethanol. As a result of measuring tyrosinase inhibitory activity for evaluating skin whitening activity, HJ6W did not show any activity. The activity at the highest concentration was 16.18% for HJ8W, 8.07% for HJ6E, and 14.7% for HJ8E. Therefore, the content of ingredients showing skin whitening activity was higher in August than in June. In the elastase inhibitory activity for evaluating the anti-wrinkle activityof the skin, the ethanol extract showed very little activity, and the hot water extract did not. In addition, since all extracts do not show astringent activity, it is judged that it is not appropriate to use HJ as a functional ingredient for preventing wrinkle formation. As a result of measuring the cell viability of HJ6E, which showed the highest polyphenol content and antioxidant activity, it showed a cell proliferation effect at low concentrationsbut strong cytotoxicity at concentrations above 50 ㎍/mL. In the case of the NO production inhibitory ability, as the concentration increased, the NO production of Raw 264.7 was suppressed. Theamount of NO production at 1,000 ㎍/mL decreased to 40.7%. However, whether these results are due to cytotoxicity or the extract's efficacy is a part that requires further research.

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표/그림 (9)

표 Table 1. The content of total phenolic compounds from Humulus japonicus extracts.
그림 Figure 1. Electron donating ability of Humulus japonicus extracts. Water (June) : Hot water extracts of Humulus japonicus collected in June, 70% ethanol (June) : 70% EtOH extracts of Humulus japonicus collected in June, Water (August) : Hot water extracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August, Vit. C : Ascorbic acid. Result are means ±S.D. of triplicate data.
그림 Figure 2. SOD-like activity of Humulus japonicus extracts. Water (June) : Hot water extracts of Humulus japonicus collected in June, 70% e thanol (June) : 70% EtOH extracts o f Humulus japonicus collected in June, Water (August) : Hot water extracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August, Vit. C : Ascorbic acid. Result are means ±S.D. of triplicate data.
그림 Figure 3. ABTS+ radical scavenging activity of Humulus japonicus extracts. Water (June) : Hot water extracts of Humulus japonicus collected in June, 70% ethanol (June) : 70% EtOH extracts of Humulus japonicus collected in June, Water (August) : Hot w ater e xtracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August, Vit. C : Ascorbic acid. Result are means ±S.D. of triplicate data.
그림 Figure 4. Tyrosinase inhibition rate of Humulus japonicus extracts. Water (June) : Hot water extracts of Humulus japonicus collected in June, 70% ethanol (June) : 70% EtOH extracts of Humulus japonicus collected in June, Water (August) : Hot water extracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August. Result are means ±S.D. of triplicate data.
그림 Figure 5. Elastase inhibition rate of Humulus japonicus extracts. Water (June) : Hot water extracts of Humulus japonicus collected in June, 70% e thanol (June) : 70% EtOH extracts o f Humulus japonicus collected in June, Water (August) : Hot water extracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August. Result are means ±S.D. of triplicate data.
그림 Figure 6. Astringent effect of Humulus japonicus e xtracts. W ater (June) : Hot w ater e xtracts o f Humulus japonicus collected in June, 7 0% e thanol (June) : 70% EtOH extracts o f Humulus japonicus collected in June, Water (August) : Hot water extracts of Humulus japonicus collected in August, 70% ethanol (August) : 70% EtOH extracts of Humulus japonicus collected in August. Result are means ±S.D. of triplicate data.
그림 Figure 7. Cell viability of extract from Humulus japonicus on melanoma cell (B16F10). After B16F10 cells were incubated for 24 h in DMEM, the cells were treated with various concentrations of Humulus japonicus for 24 h. Result are means ±S.D. of triplicate data (**p＜0.01, ***p＜0.001).
그림 Figure 8. Effect of Humulus japonicus extract on NO production in Raw 264.7 cell. Raw 264.7 cells were incubated with 1 μg/mL of LPS for 2 h and then treated with various concentrations (5, 10, 50, 100, 500 and 1,000 μg/mL) of extract from Humulus japonicus for 24 h. Result are means ±S.D. of triplicate data (**p＜0.01, ***p＜0.001).

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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