[논문]Cytotoxic Evaluation of the Essential Oils from Korean Native Plant on Human Skin and Lung Cells

AHN, Changhwan; YOO, Yeong-Min; PARK, Mi-Jin; HAM, Youngseok; YANG, Jiyoon; JEUNG, Eui-Bae

doi:10.5658/wood.2021.49.4.371

Cytotoxic Evaluation of the Essential Oils from Korean Native Plant on Human Skin and Lung Cells 원문보기

목재공학 = Journal of the Korean wood science and technology, v.49 no.4, 2021년, pp.371 - 383

AHN, Changhwan (Laboratory of Veterinary Physiology, College of Veterinary Medicine, Jeju National University) , YOO, Yeong-Min (Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University) , PARK, Mi-Jin (Division of Forest industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science) , HAM, Youngseok (Division of Forest industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science) , YANG, Jiyoon (Division of Forest industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science) , JEUNG, Eui-Bae (Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University)

Abstract ▼ AI-Helper

Plant essential oils are used in products such as fragrances and cosmetics due to their individual aromatic characteristics. Currently, essential oils are not only used in cosmetics but also in pharmaceutical products with anti-bacterial, anti-viral, anti-fungal, anti-parasitic, insecticidal, anti-cancer, neuroprotective, psychophysiological, or anti-aging effects. Despite their pharmaceutical properties, some studies reported cytotoxic effects in high doses. Therefore, for pharmaceutical purposes, the margin of safety of essential oils needs to be examined. Herein, we evaluated the IC50 of 10 essential oil from Korean native plants: Juniperus chinensis L. var. sargentii Henry, Citrus natsudaidai Hayata, Citrus reticulata Blanco, Citrus unshiu (Yu. Tanaka ex Swingle) Marcow, Artemisia capillaris Thunb, Aster glehnii F. Schmidt, Juniperus chinensis L, Zanthoxylum schinifolium Siebold & Zucc, Zanthoxylum piperitum (L.) D, and Cinnamomum loureirii. In addition, gene regulation of the cell-cycle gene and apoptosis marker CASP3 was examined at the IC50 level. The purpose of this study was to describe the toxic concentrations of essential oils extracted from Korean native plants, thereby providing toxic concentration guidelines for inclusion in a toxicity database and in the application of plant essential oils in various fields.

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표/그림 (7)

그림 Fig. 1. Cell survival curves for determining the IC₅₀ values of essential oil in the Detroit-551 cell line. Through the foregoing experiments conducted to determine the experimental concentration, the essential oil was diluted to a more detailed five concentrations; 10^-4, 10^-3, 10^-2, 10^-1, and 10^-0 (%, v/v). After the cells are treated with essential oils, the OD value was measured using the CCK assay. Cell viability was determined by OD value (treated) / OD value (untreated) × 100 (%), and the IC₅₀ value is measured through the drawn survival curve.
그림 Fig. 2. Cell survival curves for determining the IC₅₀ values of essential oil in the A549 cell line. Through the foregoing experiments conducted to determine the experimental concentration, the essential oil was diluted to a more detailed five concentrations; 10^-4, 10^-3, 10^-2, 10^-1, and 10^-0 (%, v/v). After the cells are treated with essential oils, the OD value was measured using the CCK assay. Cell viability was determined by OD value (treated) / OD value (untreated) × 100 (%), and the IC₅₀ value is measured through the drawn survival curve.
그림 Fig. 3. The expression level of CASP3 in Detroit-551 and A549 cells after treatment with essential oil. Relative expression of CASP3 in Detroit-551 (A) and A549 cell line (B). Based on the IC₅₀ values obtained above, two concentrations were set so that the difference was ten times near IC₅₀. The expression levels of CASP3 in each group were determined by real-time polymerase chain reaction after 24 hours of essential oil treatment. (Juniperus chinensis L. var. sargentii Henry; JC L., Citrus natsudaidai Hayata; CN., Citrus reticulata Blanco; CR., Citrus unshiu (Yu.Tanaka ex Swingle) Marcow; CU., Artemisia capillaris Thunb; AC., Aster glehnii F. Schmidt; AG., Juniperus chinensis L; JC., Zanthoxylum schinifolium Siebold & Zucc; ZS., Zanthoxylum piperitum (L.) DC; ZP., Cinnamomum loureirii Nees; CL.).
그림 Fig. 4. The expression level of CycllinA, Cyclin B, Cyclin D, and Cyclin E in Detroit-551 cells after treatment with essential oil. Based on the IC₅₀ values obtained above, two concentrations were set so that the difference was ten times. The expression levels Cyclin A (A), Cyclin B (B), Cyclin D (C), and Cyclin E (D) in the Detroit-551 cell line were determined by real-time polymerase chain reaction after 24 hours of essential oil treatment. The abbreviations of plant names were shown in the legend of Fig. 3.
그림 Fig. 5. The expression level of CycllinA, Cyclin B, Cyclin D, and Cyclin E in A549 cells after treatment with essential oil. Based on the IC₅₀ values obtained above, two concentrations were set so that the difference was ten times. The expression levels Cyclin A (A), Cyclin B (B), Cyclin D (C), and Cyclin E (D) in the A549 cell line were determined by real-time polymerase chain reaction after 24 hours of essential oil treatment. The abbreviations of plant names were shown in the legend of Fig. 3.
그림 Fig. 6. The expression level of Cyclin A, Cyclin B, Cyclin D, and Cyclin E in Detroit-551 cells after treatment with essential oil. Based on the IC₅₀ values obtained above, the higher dose of essential oil 10^-2 (%, v/v) was chosen to examine protein expression of cell cycle markers and cell apoptosis marker gene. The expression levels Cyclin A, Cyclin B, Cyclin D, Cyclin E, and Caspase-3 in each group were determined by western blotting after 24 hrs of essential oil treatment. The abbreviations of plant names were shown in the legend of Fig. 3.
표 Table 1. Physical properties of the essential oil from Korean native plants

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