[논문]감초 추출물 함유 키토산 나노캡슐의 특성 및 항산화 활성

김민정; 이지수; 이현규

doi:10.9721/kjfst.2021.53.4.479

감초 추출물 함유 키토산 나노캡슐의 특성 및 항산화 활성
Characteristics of Glycyrrhiza uralensis extract-loaded chitosan nanocapsules and their antioxidant activity 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.4, 2021년, pp.479 - 485

김민정 (한양대학교 식품영양학과) , 이지수 (한양대학교 식품영양학과) , 이현규 (한양대학교 식품영양학과)

초록
AI-Helper

감초 추출물의 항산화 안정성을 증진시키기 위하여 키토산의 ionic gelation 특성을 이용한 감초 추출물을 함유한 키토산/TPP 나노캡슐을 제조하였다. 먼저 반응표면분석을 통해서 감초 추출물의 항산화활성을 최대로 할 수 있는 추출조건을 최적화하였다. 감초의 최적 추출조건은 83.01% 에탄올 농도에서 32.6분 동안 추출하는 것으로 결정되었으며, 최적 조건에서 제조된 감초 추출물의 총 플라보노이드 함량과 항산화 활성은 각각 20.47 ㎍ RE/mg과 22.32%/mg로 관측되어 예측값과 모두 표준편차 범위 내에 있음을 확인하였다. 다양한 감초 추출물과 키토산 농도에서 나노캡슐을 제조한 결과, 약 280-370 nm 범위의 캡슐크기를 나타냈으며 0.3 이하의 PDI를 나타내어 전반적으로 균일한 나노캡슐이 형성 되는 것으로 확인되었다. 포집효율 및 함유효율을 고려하여 감초 추출물과 키토산 농도를 각각 0.8과 2.0 mg/mL로 결정하였다. 지방과산화 억제능을 통한 APF 측정 결과, 나노캡슐화되지 않은 감초추출물은 반응 후에 급격히 상승되어 반응 4시간에 가장 높은 APF를 나타낸 후 급격히 감소된 반면, 감초 추출물 나노캡슐은 초반에는 약한 APF를 나타내다가 꾸준히 증가한 후 유지되어 반응 6시간 이후에는 모든 농도에서 유리 감초 추출물보다 유의적으로 높은 항산화 활성을 나타냈다. 따라서 감초 추출물은 키토산 나노캡슐화에 의해서 산화 안정성이 향상됨으로써 항산화 활성의 유지기간이 증진됨을 확인하였다. 본 연구를 통해서 키토산 나노캡슐화는 감초추출물의 항산화 안정성 증대에 효과적으로 활용될 수 있을 것으로 판단된다.

Abstract ▼ AI-Helper

To improve the oxidative stability of Glycyrrhiza uralensis extract (GU), GU extraction conditions were optimized for maximal antioxidant activity, and GU-loaded nanocapsules were prepared by chitosan ionic gelation. The optimized ethanol concentration and extraction time were 83.0% and 32.6 min, respectively, using response surface methodology. The particle size of the GU-loaded nanocapsules ranged from 280 to 370 nm. A GU extract of 0.8 mg/mL and chitosan concentration of 2.0 mg/mL were selected as the optimal conditions for entrapment and loading efficiency. Both free GU and GU-loaded chitosan nanocapsules exhibited concentration-dependent antioxidant activity. However, the antioxidant protection factor of GU was effectively maintained when it was entrapped within the chitosan nanocapsules. In conclusion, chitosan nanoencapsulation is a potentially valuable technique for improving the oxidative stability of GU.

주제어

표/그림 (7)

표 Table 1. Experimental values of total flavonoid content and DPPH radical-scavenging activity determined from ten experimental runs generated by the central composite design
그림 Fig. 1. Three-dimensional response surface plots for effects of ethanol concentration and extraction time on (A) total flavonoid content and (B) DPPH radical scavenging activity of G. uralensis extracts.
표 Table 2. Optimized values obtained by maximizing DPPH radical-scavenging activity
그림 Fig. 2. Linear regression analyses between DPPH radical scavenging activity and total flavonoid content of G. uralensis extracts.
그림 Fig. 3. Particle size and PDI of chitosan nanocapsules with different concentrations of G. uralensis extract (A) and chitosan (B). Different letters (a-b) indicate a significant difference in particle size (chitosan=2.0 mg/mL, TPP=1.6 mg/mL (A) and G. uralensis extract=1.0 mg/mL, TPP=1.6 mg/mL (B)).
그림 Fig. 4. Entrapment and loading efficiency of chitosan nanocapsules with different concentrations of G. uralensis extract (A) and chitosan (B). Different letters (A-C and a-c) indicate a significant different LE or EE, respectively (chitosan=2.0 mg/mL, TPP=1.6 mg/mL (A) and G. uralensis extract=1.0 mg/mL, TPP=1.6 mg/mL (B)).
그림 Fig. 5. Antioxidant protection factor of free G. uralensis extract and G. uralensis extract-loaded chitosan nanocapsules by β-carotene bleaching assay.

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저자의 다른 논문 :

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동의어: Packet Collision Rate

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