[논문]보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과

양지영; 함현미; 이현진; 김현영; 우소연; 서우덕; 이미자

doi:10.9799/ksfan.2021.34.5.423

보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과
Antioxidative and Hepatoprotective Effects of Ethanol Extracts from Different Barley Cultivars 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.5, 2021년, pp.423 - 429

양지영 (농촌진흥청 국립식량과학원 작물기초기반과) , 함현미 (농촌진흥청 국립식량과학원 작물기초기반과) , 이현진 (농촌진흥청 국립식량과학원 작물기초기반과) , 김현영 (농촌진흥청 국립식량과학원 작물기초기반과) , 우소연 (농촌진흥청 국립식량과학원 작물기초기반과) , 서우덕 (농촌진흥청 국립식량과학원 작물기초기반과) , 이미자 (농촌진흥청 국립식량과학원 작물기초기반과)

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Barley's nutritional value as a health food is increasing due to its excellent nutritional functionality. In this study, the levels of β-glucan, total polyphenols, and total flavonoids were analyzed in the ethanol extracts of different barley cultivars (Hinchalssal, Heuksoojeongchal, Betaone, Ganghochung, and Saechalssal). Also, the free radical scavenging abilities of 2,2-diphenyl-1-picryl-hydrazil (DPPH) and 2,2'-azino-bis-3-ethylbenzo-thiaxoline-6-sulfonic acid (ABTS) were measured to determine their antioxidant activity. The results confirmed that Betaone extract contained highly activefunctional components and exhibitedantioxidant activity. Next, we evaluated the hepatoprotective and inhibitory effects of reactive oxygen species (ROS) generated by barley ethanol extracts after inducing oxidative stress with tert-butyl hydroperoxide (tBHP) in HepG2 cells. Hinchalssal and Saechalssal extracts showed the most significant cytoprotective effect and also reduced ROS production significantly. These results suggest that Hinchalssal, Saechalssal, and Betaone represent potential natural antioxidant and hepatoprotective agents.

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표/그림 (6)

표 Table 1. β-Glucan, total phenol and total flavonoid content of ethanol extracts of barley cultivar
그림 Fig. 1. Effect of barley ethanol extracts on viability of HepG2 cells. HepG2 cells were treated with barely ethanol extracts at (a) 20 μg/mL, (b) 50 μg/mL and (c) 100 μg/mL concentrations for 24 hours and the relative cell viability was assessed by EZ-Cytox cell viability assay. Data are presented as the mean±S.D. of three independent experiments. N.S. means no significant difference between all treatment group. Abbreviations: C, vehicle only; No. 1, Hinchalssal; No. 2, Heuksoojeongchal; No. 3, Betaone; No. 4, Ganghochung; No. 5, Saechalssal.
표 Table 2. Radical scavenging activity of ethanol extracts of barley cultivar
그림 Fig. 2. Protective effect of barely ethanol extracts on against tBHP-induced cytotoxicity in HepG2 cells. HepG2 cells were treated with 0.5 mM tBHP for 2 hours after pre-treatment with barely ethanol extracts at (a) 20 μg/mL, (b) 50 μg/mL and (c) 100 μg/mL concentrations for 20 hours. Data are presented as the mean±S.D. of three independent experiments. Different small letters in the same items indicate a significant difference (p<0.05) compared to control. Abbreviations: C, vehicle only; T, vehicle + tBHP only; No. 1, Hinchalssal; No. 2, Heuksoojeongchal; No. 3, Betaone; No. 4, Ganghochung; No. 5, Saechalssal.
그림 Fig. 3. Effect of Hinchalssal and Saechalssal on the generation of intracellular ROS levels in tBHP-treated HepG2 cells. HepG2 cells were treated with 0.5 mM tBHP for 3 hours after pre-treatment with or without 50 μg/mL extracts for 20 hours. After staining with DCF-DA florescent dye, DCF fluorescence intensity was measured using a fluorescence microplate reader. Data are presented as the mean± S.D. of three independent experiments. Different small letters in the same items indicate a significant difference (p<0.05) compared to control. Abbreviations: C, vehicle only; T, vehicle + tBHP only; No. 1, Hinchalssal; No. 5, Saechalssal; Q, quercetin 20 μM.
표 Table 3. Correlation coefficients between chemical composition, radical scavenging activity and protective effect of barely ethanol extracts on against tBHP-induced cytotoxicity in HepG2 cells

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보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과
Antioxidative and Hepatoprotective Effects of Ethanol Extracts from Different Barley Cultivars 원문보기

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보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과 Antioxidative and Hepatoprotective Effects of Ethanol Extracts from Different Barley Cultivars 원문보기

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보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과
Antioxidative and Hepatoprotective Effects of Ethanol Extracts from Different Barley Cultivars 원문보기

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