[논문]은행나무 종자 추출물의 미백효능 분석

최은영; 장영아

doi:10.12925/jkocs.2021.38.5.1229

은행나무 종자 추출물의 미백효능 분석
The Analysis of Whitening Effects on Extracts from Ginkgo (Ginkgo biloba L.) Seeds 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.5, 2021년, pp.1229 - 1240

최은영 (대구한의대학교 바이오산업융합학부) , 장영아 (경성대학교 스마트헬스케어융복합연구센터)

초록
AI-Helper

은행나무 종자인 백과를 70% 에탄올로 추출하여 미백 효능을 규명하고 화장품 소재로서의 응용 가능성을 확인하고자 하였다. 백과 에탄올추출물 (GBE)을 B16F10 melanoma cells에 처치하여 멜라닌 생성과 tyrosinase 활성을 확인한 결과 유의한 수준의 멜라닌 생성 저해가 관찰되었고, 멜라닌 생성과정에 관여하는 주요 효소인 tyrosinase의 활성이 농도 의존적으로 억제됨이 관찰되었다. 멜라닌 생성 관련 주요 인자들의 단백질 발현과 mRNA 수준을 관찰한 결과, tyrosinase, tyrosinase-related protein-1, -2 (TRP-1, -2), microphthalmia-associated transcription factor (MITF)의 단백질 발현과 유전자 수준이 GBE의 처리에 의해 유의한 수준으로 저해되었다. 이 결과를 통해, 본 연구의 백과 에탄올추출물은 멜라닌 세포의 멜라닌 생성 관련 핵심 전사인자인 MITF의 조절을 통해 멜라닌 생성 억제 효과를 나타내는 것으로 보인다. 이에 따라 백과 에탄올추출물을 화장품 미백 기능성 소재로 활용 가능성이 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Ginkgo (Ginkgo biloba L.) seeds, called 'Baekqwa', were extracted from 70% ethanol to investigate the whitening effect and to confirm the application potential as a cosmetic material. Ginkgo seed ethanol extracts (GBE) were treated with B16F10 melanoma cells, and melanin contents and tyrosinase, which is the main enzyme concerning the synthesis process of melanin, inhibitory activity were confirmed. As a result, there were inhibited in a concentration-dependent manner, and GBE also significantly reduced the protein expression and mRNA levels of tyrosinase, tyrosinase-related protein-1, -2 (TRP-1, -2), and their upstream transcription factor, microphthalmia-associated transcription factor (MITF). These results suggest that GBE could be used as an effective whitening agent that has an inhibitory effect on melanin production by regulating the expression and degradation of MITF in melanocytes.

주제어

표/그림 (7)

그림 Fig. 1. DPPH radical scavenging activity (A) and ABTS⁺ radical scavenging activity (B) of GBE. GBE : 70% ethanol extract of Ginkgo seeds, Vit.C : positive control. Results are mean±SD of triplicate data. Means with different letters (a-i) above the bars are significantly different by Duncan's multiple range test at p<0.05.
그림 Fig. 2. Tyrosinase inhibition activity of GBE. GBE : 70% ethanol extract of Ginkgo seeds, Vit.C : positive control. Results are mean±SD of triplicate data. Means with different letters (a-g) above the bars are significantly different by Duncan’s multiple range test at p<0.05.
그림 Fig. 3. Cell viability assay on B16F10 melanoma cells of GBE. The cells were treated for 24 hr with the indicated concentrations of GBE. GBE : 70% ethanol extract of Ginkgo seeds, Cont : not treated any extract, Results aremeans±S.D. of triplicate data. One-way ANOVA was used for comparisons of multiple group means followed by t-test (significant as compared to control ; *p<0.05).
그림 Fig. 4. Melanin contents in α-MSH stimulated B16F10 melanoma cells. The cells were treated with various concentrations of GBE for 48 hr after treatment with α-MSH (1 nM) for 4 hr. GBE : 70% ethanol extract of Ginkgo seeds, Non : not treated any extract and α-MSH, Cont : only treated α-MSH (positive control). Results are means±S.D. of triplicate data. One-way ANOVA was used for comparisons of multiple group means followed by t-test (significant as compared to control ; ^*p<0.05).
그림 Fig. 5. Tyrosinase inhibition activity in α-MSH stimulated B16F10 cells. The cells were treated with various concentrations of GBE for 48 hr after treatment with α-MSH (1 nM) for 4 hr. GBE : 70% ethanol extract of Ginkgo seeds, Non : not treated any extract and α-MSH, Cont : only treated α-MSH (positive control). Results are means±S.D. of triplicate data. One-way ANOVA was used for comparisons of multiple group means followed by t-test (significant as compared to control ; ^* p<0.05).
그림 Fig. 6. The expression of melanogenesis-related proteins in α-MSH stimulated B16F10 melanoma cells from GBE. The cells were treated with various concentrations of GBE for 24 hr before treatment with α-MSH (1 nM) for 1 hr. And the cells were incubated for 48 hr and then the protein expressions of Tyrosinase, MITF, TRP-1, and TRP-2 were analysed by western blot assay. GBE : 70% ethanol extract of Ginkgo seeds, Cont : not treated any extract and α-MSH, α-MSH : positive control. Results are means±S.D. of triplicate data. One-way ANOVA was used for comparisons of multiple group means followed by t-test (significant as compared to control ; ^* p<0.05).
그림 Fig. 7. The expression of melanogenesis-related mRNA in α-MSH stimulated B16F10 melanoma cells from GBE. The cells were treated with various concentrations of GBE for 24 hr before treatment with α-MSH (1 nM) for 1 hr. And the cells were incubated for 48 hr and then the mRNA expressions of Tyrosinase, MITF, TRP-1, and TRP-2 were analysed by real-time PCR. GBE : 70% ethanol extract of Ginkgo seeds, Cont : not treated any extract and α-MSH, α-MSH : positive control. Results are means±S.D. of triplicate data. One-way ANOVA was used for comparisons of multiple group means followed by t-test (significant as compared to control ; ^* p<0.05).

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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