[논문]백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향

박석만; 이세희; 정다운; 조수정; 신미래; 박해진; 노성수

doi:10.6116/kjh.2022.37.1.19.

백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향
Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.1, 2022년, pp.19 - 29

박석만 (대구한의대학교 한의과대학 본초약리학교실) , 이세희 (대구한의대학교 한의과대학 본초약리학교실) , 정다운 (대구한의대학교 간질환한약융복합활용연구센터) , 조수정 (대구한의대학교 간질환한약융복합활용연구센터) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (대구한의대학교 DHU 바이오융복합시험센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

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표/그림 (8)

그림 Figure. 1. Total polyphenol and total flavonoid contents of AM All values are expressed as mean±SEM of 3 replications. AM; Atractylodes macrocephala Koidzumi water extract.
그림 Figure. 2. Scavenging activity of AM on DPPH and ABTS radical. All values are expressed as mean±SEM of 3 replications. AM: Atractylodes macrocephala Koidzumi water extract.
그림 Figure. 3. Body weight change. All data are expressed mean±SD (n=8 per group). AM; Atractylodes macrocephala Koidzumi water extract. Animals were divided into 4 groups: normal mice (Normal), 5% DSS-induced ulcerative colitis mice (Control), 5% DSS-induced ulcerative colitis mice treated with AM 100 mg/kg body weight mice (AM100), 5% DSS-induced ulcerative colitis mice treated with AM 200 mg/kg body weight mice (AM200). Significance: ^##p < 0.01 compared vs. Normal group.
그림 Figure. 4. Macroscopic length changes of colon. All data are expressed mean±SD (n=8 per group). AM; Atractylodes macrocephala Koidzumi water extract. Animals were divided into 4 groups: normal mice (Normal), 5% DSS-induced ulcerative colitis mice (Control), 5% DSS-induced ulcerative colitis mice treated with AM 100 ㎎/㎏ body weight mice (AM100), 5% DSS-induced ulcerative colitis mice treated with AM 200 mg/kg body weight mice (AM200). Significance: ^###p < 0.001 compared vs. Normal group.
표 Table 1. Indicators of biochemical analysis of serum and colon.
그림 Figure. 5. Expressions of NADPH-related proteins in colon. A) NADPH oxidase 2 (NOX2); B) P22phox, C) iNOS. All data are expressed mean±SD (n=8 per group). AM; Atractylodes macrocephala Koidzumi water extract. Animals were divided into 4 groups: normal mice (Normal), 5% DSS-induced ulcerative colitis mice (Control), 5% DSS-induced ulcerative colitis mice treated with AM 100 mg/kg body weight mice (AM100), 5% DSS-induced ulcerative colitis mice treated with AM 200 mg/kg body weight mice (AM200). Significance: ^#p < 0.05 compared vs. Normal group and *p < 0.05, vs. Control group.
그림 Figure. 6. Expressions of antioxidant-related proteins in colon. A) SOD; B) Catalase; C) GPx-1/2. All data are expressed mean±SD (n=8 per group). AM; Atractylodes macrocephala Koidzumi water extract. Animals were divided into 4 groups: normal mice (Normal), 5% DSSinduced ulcerative colitis mice (Control), 5% DSS-induced ulcerative colitis mice treated with AM 100 mg/kg body weight mice (AM100), 5% DSS-induced ulcerative colitis mice treated with AM 200 mg/kg body weight mice (AM200). Significance: ^#p < 0.05 compared vs. Normal group and *p < 0.05 vs. Control group.
그림 Figure. 7. Histopathological analysis. H&E staining of colon (magnification × 200 / scale bar = 100 μm). Blue arrows were mean Inflammation cellular infiltration in large intestine tissue. AM; Atractylodes macrocephala Koidzumi water extract. A; Normal, normal mice, B; Control, 5% DSS-induced ulcerative colitis mice, C; AM100, 5% DSS-induced ulcerative colitis mice treated with AM extract 100 mg/kg body weight, AM200; 5% DSS-induced ulcerative colitis mice treated with AM 200 mg/kg body weight.

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