[논문]AMPK/Sirt1/PGC-1α 신호 전달 경로의 조절을 통한 오미자 추출물의 비만 개선 효과

이세희; 박해진; 신미래; 노성수

doi:10.6116/kjh.2022.37.2.1.

AMPK/Sirt1/PGC-1α 신호 전달 경로의 조절을 통한 오미자 추출물의 비만 개선 효과
Anti-Obesity Effect of Schizandrae Fructus Water Extract through Regulation of AMPK/Sirt1/PGC-1α signaling pathway 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.2, 2022년, pp.1 - 11

이세희 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (대구한의대학교 DHU바이오융복합시험센터) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objectives : Although the anti-obesity effect of Schizandrae Fructus water extract has been demonstrated, its underlying mechanism is still unclear. Therefore, we aimed to evaluate the anti-obesity effect of Schizandrae Fructus water extract through the p-AMP-activated protein kinase (p-AMPK), sirtuin1 (Sirt1), and peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) signaling in 60% high-fat diet (HFD)-induced obese mouse model. Methods : Male C57BL/6 mice were divided into four groups. The Normal group was fed a normal diet and the obese groups were fed 60% HFD. Except for the Control group, the GG group was supplemented with 0.5% Garcinia gummigutta and the SCW group was supplemented with 0.5% Schizandrae Fructus water extract. After 6 weeks, obesity-related biomarkers in serum were measured and the expressions of protein for lipid-related factors in liver tissue were analyzed by western blot. Results : Treatment with SCW significantly down-regulated body weight compared to the Control group. SCW down-regulated levels of triglyceride and total cholesterol in serum and significantly increased p-AMPK, Sirt1, and PGC-1α in liver tissue. In addition, the expressions of fatty acid oxidation-related proteins such as peroxisome proliferator-activated receptor α (PPARα), carnitine palmitoyltransferase 1A (CPT-1A), uncoupling protein 1 (UCP1), and uncoupling protein 3 (UCP3) were significantly up-regulated. However, fatty acid synthesis-related proteins including sterol regulatory element-binding protein-1 (SREBP-1), phospho-Acetyl-CoA Carboxylase (p-ACC), and fatty acid synthase (FAS) were significantly down-regulated. Conclusions : Taken together, SCW treatment showed anti-obesity effect by regulating both fatty acid oxidation-related and fatty acid synthesis-related proteins through AMPK/Sirt1/PGC-1α signaling in 60% HFD-induced obese mice.

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표/그림 (7)

그림 Fig. 1. Scavenging activity of Schizandrae Fructus water extract on DPPH and ABTS radical. All values are expressed as mean ± SEM of 3 replications.
그림 Fig. 2. Body weight change, and food efficiency ratio in 60% HFD-fed Obese mice. All data are expressed mean ± SD (n=8 per group). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract. Significance: ^###p < 0.001 versus Normal group and *p < 0.05, **p < 0.01 versus Control group.
그림 Fig. 3. Effects of Schizandrae Fructus water extract on serum TG and TC levels in 60% HFD-fed obese mice. All data are expressed mean ± SD (n=8 per group). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract. Significance: ^###p < 0.001 versus Normal group and ***p < 0.001 versus Control group.
그림 Fig. 4. Effects of Schizandrae Fructus water extract on AMPK/Sirt1/PGC-1α proteins in liver tissue. All data are expressed mean ± SD (n=8 per group). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract. Significance: ^#p < 0.05 and ^##p < 0.01 versus Normal group and *p < 0.05, **p < 0.01 versus Control group.
그림 Fig. 5. Effects of Schizandrae Fructus water extract on fatty acid oxidation related proteins in liver tissue. All data are expressed mean ± SD (n=8 per group). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract. Significance: ^##p < 0.01 versus Normal group and *p < 0.05, **p < 0.01 versus Control group.
그림 Fig. 6. Effects of Schizandrae Fructus water extract on fatty acid synthesis related proteins in liver tissue. All data are expressed mean ± SD (n=8 per group). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract. Significance: ^##p < 0.01, ^###p < 0.001 versus Normal group and *p < 0.05, **p < 0.01 versus Control group.
그림 Fig. 7. Effect of Schizandrae Fructus water extract on histopathological analysis in liver tissue. (Original magnification × 200, magnification bar = 100 μm). Mice were divided into 4 groups: Normal, normal mice; Control, 60% HFD-fed mice; GG, the mice fed HFD supplemented with 0.5% Garcinia gummi-gutta; SCW, the mice fed HFD supplemented with 0.5% Schizandrae Fructus water extract.

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