[논문]두충 추출물의 류마티스관절염 동물모델에 대한 개선 효과

지중구

doi:10.12925/jkocs.2022.39.1.18

두충 추출물의 류마티스관절염 동물모델에 대한 개선 효과
Improvement Effect of the Eucommia ulmoides Extracts on CIA-induced Rheumatoid Arthritis Animal Models 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.39 no.1, 2022년, pp.18 - 26

초록
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본 연구는 CIA에 의해 유발된 DBA/1 마우스를 통해 류마티스관절염 바이오마커에 대한 두충 추출물의 효과를 평가하였다. 평가를 위해 류마티스관절염 동물모델 제작 후 100 mg/kg/day로 4주간 경구 투여하고 혈청 바이오마커, 방사선, 구조적 매개변수 분석을 통해 치료 효과를 확인하였다. 음성대조군과 비교하여, 두충 추출물은 염증 및 면역글로불린 마커(TNF-α, IgG 및 hs-CRP)의 생성을 유의하게 감소시켰고, 백혈구의 단핵구 수를 유의적으로 감소시켰다. 또한, 두충 추출물은 관절 파괴를 효과적으로 보존하고 관절 변형을 줄였으며, 골 밀도의 증가와 유의적으로 골 염증을 감소시켰다. 이러한 결과는 두충 추출물이 류마티스관절염 증상을 개선하는 것을 나타낸다. 따라서, 두충 추출물은 류마티스관절염 관리를 위한 새로운 치료 옵션이 될 수 있음을 보여준다.

Abstract ▼ AI-Helper

The present study aimed to evaluate the effect of Eucommia ulmoides extracts on rheumatoid arthritis biomarker in a CIA-induced DBA/1 mice. For evaluation, Eucommia ulmoides extracts was administered orally at dose of 100 mg/kg/day for 4 weeks after production of an animal model of rheumatoid arthritis and we confirmed the treatments' effects based on serum biomarker, radiological, structural parameter analysis. Compared to the negative control group, the Eucommia ulmoides extracts treatments significantly reduced the serum level of inflammation and immunoglobulin markers (i.e., TNF-α, IgG, and hs-CRP), and significantly decreased the monocyte count of white blood cells. Furthermore, the Eucommia ulmoides extracts treatments effectively preserved the joint destruction, and little the joint deformation. Moreover, compared to the negative control group, the Eucommia ulmoides extracts treatments increased the bone volume, and significantly decreased bone inflammation. The results indicate that the Eucommia ulmoides extracts improved rhrumatoid arthritis symptoms. Thus, the Eucommia ulmoides extracts may be a novel therapeutic option for the management of rheumatoid arthritis.

주제어

표/그림 (5)

그림 Fig. 1. Effects of Eucommia ulmoides extracts on IL-6(A), IL-17(B), and TNF-α(C) level in CIA-induced DBA/1 mice. The measured levels were expressed as the mean±standard deviation (n = 8/group). ***p<0.001, **p<0.01 indicates a significant difference from the control group. Normal, non treatment group; Control, rueumatoid arthritis group; Indo, indomethacin was administered orally at doses of 2.5 mg/kg/day; EDU, Eucommia ulmoides extracts was administered orally at doses of 100 mg/kg/day.
그림 Fig. 2. Effects of Eucommia ulmoides extracts on IgG level in CIA-induced DBA/1 mice. The measured levels were expressed as the mean±standard deviation (n = 8/group). **p<0.01 indicates a significant difference from the control group. Normal, non treatment group; Control, rueumatoid arthritis group; Indo, indomethacin was administered orally at doses of 2.5 mg/kg/day; EDU, Eucommia ulmoides extracts was administered orally at doses of 100 mg/kg/day.
그림 Fig. 3. Effects of Eucommia ulmoides extracts on monocyte counting(A), and hs-CRP(B) level in CIA-induced DBA/1 mice. The measured levels were expressed as the mean±standard deviation (n = 8/group). **p<0.01 indicates a significant difference from the control group. Normal, non treatment group; Control, rueumatoid arthritis group; Indo, indomethacin was administered orally at doses of 2.5 mg/kg/day; EDU, Eucommia ulmoides extracts was administered orally at doses of 100 mg/kg/day.
그림 Fig. 4. Effects of Eucommia ulmoides extracts on the micro CT in CIA-induced DBA/1 mice. Representative morphological images of the right paw was obtained by three-dimension micro-computed tomography. Normal, non treatment group; Control, rueumatoid arthritis group; Indo, indomethacin was administered orally at doses of 2.5 mg/kg/day; EDU, Eucommia ulmoides extracts was administered orally at doses of 100 mg/kg/day.
그림 Fig. 5. Effects of Eucommia ulmoides extracts on bone volume(A), and bone surface/bone volume(B) ratio in CIA-induced DBA/1 mice. The measured levels were expressed as the mean±standard deviation (n = 8/group). *p<0.05 indicates a significant difference from the control group. BV; bone volume, BS; bone surface. Normal, non treatment group; Control, rueumatoid arthritis group; Indo, indomethacin was administered orally at doses of 2.5 mg/kg/day; EDU, Eucommia ulmoides extracts was administered orally at doses of 100 mg/kg/day.

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