[논문]제2형 당뇨 동물모델에서 일반메밀과 쓴메밀의 항당뇨 효과 비교

김수정; 손황배; 최지명; 조은주; 남정환; 이종남; 서종택; 장동칠; 김율호

doi:10.9721/kjfst.2022.54.1.17

제2형 당뇨 동물모델에서 일반메밀과 쓴메밀의 항당뇨 효과 비교
Anti-diabetic effects of common buckwheat and tartary buckwheat in type II diabetes animal model 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.54 no.1, 2022년, pp.17 - 27

김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) , 손황배 (농촌진흥청 국립식량과학원 고령지농업연구소) , 최지명 (부산대학교 식품영양학과 및 김치연구소) , 조은주 (부산대학교 식품영양학과 및 김치연구소) , 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) , 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) , 서종택 (농촌진흥청 국립식량과학원 고령지농업연구소) , 장동칠 (바이오에너지 작물연구소) , 김율호 (농촌진흥청 국립식량과학원 중부작물과)

초록
AI-Helper

메밀은 전 세계적으로 곡식, 싹, 차 등 다양한 형태로 이용되고 있으며, 주요 재배종으로는 일반메밀과 쓴메밀이 있다. 본 연구는 일반메밀과 쓴메밀 종실과 전초를 대상으로 항산화 및 항당뇨 활성 효과를 평가하였다. 루틴 함량은 쓴메밀 추출물이 일반메밀 추출물보다 44-48배 이상 높았으며, 퀘세틴은 전초에서만 검출되었다. 플라보노이드 및 폴리페놀 함량도 쓴메밀 추출물이 일반메밀 추출물보다 2.5-4.8배 높았다. STZ 투여로 제2형 당뇨가 유발된 동물모델에 일반메밀과 쓴메밀의 종실과 전초를 급여하여 공복혈당, 경구 내당능, 인슐린 내성 변화, 혈중 포도당 및 인슐린 농도 등을 통해 항당뇨 효과를 확인하였다. 종실의 경우는 일반메밀에 비해 쓴메밀 종실군에서 경구 내당능 효과가 뛰어났으며, 혈청 포도당 농도도 유의적으로 감소하는 것을 확인하였다. 전초의 경우, 일반메밀이나 쓴메밀 급여 실험군에서 모두 당뇨대조군에 비하여 유의적인 항당뇨 효과를 보였으며, 인슐린 저항성 지표인 HOMA-IR과 인슐린 민감성 지표인 QUICKI를 비교해본 결과에서도 종실과 전초 섭취군 모두 인슐린 저항성을 감소시키고, 인슐린 민감성을 증가시키는 것을 확인하였다. 이러한 결과들을 고려하면 루틴 함량 및 항산화활성이 쓴메밀 전초의 경우 항당뇨 효과와 밀접한 관계가 있으며, 루틴 함량과 항산화 활성이 높은 쓴메밀 종실 및 일반메밀과 쓴메밀 전초는 항당뇨 식의약 소재로 활용 가능성이 높은 것으로 판단된다.

Abstract ▼ AI-Helper

In this study, we evaluated the antioxidant and antidiabetic effects of buckwheat. The diabetic animal models were divided into four groups: normal mice group (NOR), streptozotocin-induced diabetic mice group (STZ), group treated with seeds of common or tartary buckwheat (SCB or STB), and the group treated with whole plants of common or tartary buckwheat (PCB or PTB). Rutin content was 44-48 times higher in STB or PTB than in SCB. Oral glucose tolerance and insulin resistance were significantly reduced by treatment with STB, PCB, and PTB. Treatment with PTB also decreased the serum glucose level significantly and the serum insulin levels slightly compared with the STZ group. These results suggest that rutin content and antioxidant activity are closely related to the antidiabetic effect of the treatment. Our results demonstrate that the seeds of tartary buckwheat and whole plants of either common or tartary buckwheat have antidiabetic effects-attenuating blood glucose in an animal model of type II diabetes.

주제어

표/그림 (10)

그림 Fig. 1. Seed and plant of common buckwheat (Fagopyrum esculentum Moench) and tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). A, seed of common buckwheat (SCB); B, seed of tartary buckwheat (STB); C, whole plant of common buckwheat in flowering time (PCB); D, whole plant of tartary buckwheat in flowering time (PTB).
그림 Fig. 2. Schedule for treatment of mice in experiment for 10 weeks. Type 2 diabetic mice were divided into each group: NOR, STZ, SCB, STB, PCB, and PTB. HFD refer to high fat diet; NOR, normal mice group (normal rats+basal control diet); STZ, streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat), seed or whole plant powder of common buckwheat treated group; STB or PTB, seed or plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat); FBG, fasting blood glucose; ITT, insulin tolerance test; OGTT, oral glucose tolerance test; SG & SI, serum glucose & serum insulin.
표 Table 1. Composition of diet-control, fat diet, and buckwheat diet (unit: g/kg)
표 Table 2. Rutin and quercetin contents in seed and whole plant from common buckwheat and tartary buckwheat.
그림 Fig. 3. Boxplots of anti-oxidant activities in seed and whole plant from common buckwheat and tartary buckwheat based on material parts, including DPPH (A), ABTS (B), flavonoid (C), and polyphenol contents (D). Material of buckwheat was cultivated in Deagwallyeong, Pyeongchang. SCB, seed of common buckwheat; STB, seed of tartary buckwheat; PCB, whole plant of common buckwheat in flowering time; PTB, whole plant of tartary buckwheat in flowering time. The levels of DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS radical scavenging activity, flavonoid and polyphenol content in the extractions were determined by a multi-mode microplate reader, respectively. All microplate images passed through multimode reader at standard solution, which are as follows: (1) DPPH standard (0.00-0.083 mg/mL, yellow-purple); (2) ABTS standard (0.00-0.083 mg/mL, white-turquoise); (3) Flavonoid standard (0.00- 0.400 mg/mL, white-orange); and (4) Polyphenol standard (0.00- 0.400 mg/mL, white-molybdenum blue). UV/Vis absorbance detectors of anti-oxidants are as follows: DPPH (520 nm); (2) ABTS (734 nm); (3) Flavonoid (510 nm); and (4) Polyphenol (750 nm). Data shown are the mean±SD (n=6). In each categorized aberration, different letters indicate statistically significant differences (p<0.05, Duncan's multiple range test).
그림 Fig. 4. Changes in fasting blood glucose of STZ-induced diabetic mice fed with experimental diets of buckwheat seed (A) and whole plant (B). NOR (○), Normal mice group (normal rats+basal control diet); STZ (■), Streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB (▲), seed or whole plant powder of common buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat); STB or PTB (◆), seed or whole plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat). Mean±SD (n=6) with different superscripts at the top of bar represent that they are significantly different according to DMRT at α=0.05. Superscript "a" indicates the highest value while "c" indicates the lowest value. The standard bar in the figure represents the standard deviation.
그림 Fig. 5. Effects of buckwheat seed (A) and whole plant (B) intake on oral glucose tolerance test in STZ-injected mice. NOR (○), Normal mice group (normal rats+basal control diet); STZ (■), Streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB (▲), seed or whole plant powder of common buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat); STB or PTB (◆), seed or whole plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat). Mean±SD (n=6) with different superscripts at the top of bar represent that they are significantly different according to DMRT at α=0.05. Superscript "a" indicates the highest value while "c" indicates the lowest value. The standard bar in the figure represents the standard deviation.
그림 Fig. 6. Effects of buckwheat seed (A) and whole plant (B) intake on insulin tolerance test (ITT) in STZ-injected mice. NOR (○), Normal mice group (normal rats+basal control diet); STZ (■), Streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB (▲), seed or whole plant powder of common buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat); STB or PTB (◆), seed or whole plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat). Mean±SD (n=6) with different superscripts at the top of bar represent that they are significantly different according to DMRT at α=0.05. Superscript "a" indicates the highest value while "c" indicates the lowest value. The standard bar in the figure represents the standard deviation.
그림 Fig. 7. Effects of buckwheat seed (A, C) and whole plant (B, D) intake on serum glucose and insulin level test in STZ-injected mice. NOR, Normal mice group (normal rats+basal control diet); STZ, Streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB, seed or whole plant powder of common buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat); STB or PTB, seed or whole plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat). Mean±SD (n=6) with different superscripts at the top of bar represent that they are significantly different according to DMRT at α=0.05. Superscript "a" indicates the highest value while "c" indicates the lowest value. The standard bar in the figure represents the standard deviation.
그림 Fig. 8. Effects of buckwheat seed (A, C) and whole plant (B, D) intake on HOMA-IR and QUICKI level test in STZ-injected mice. NOR , Normal mice group (normal rats+basal control diet); STZ, Streptozotocin-induced diabetic mice group (Streptozotocin-induced diabetic rats+high fat diet with 60 calories); SCB or PCB, seed or whole plant powder of common buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of common buckwheat); STB or PTB, seed or whole plant powder of tartary buckwheat treated group (Streptozotocin-induced diabetic rats+feed diet containing 10% seed or whole plant powder of tartary buckwheat). Mean±SD (n=6) with different superscripts at the top of bar represent that they are significantly different according to DMRT at α=0.05. Superscript "a" indicates the highest value while "c" indicates the lowest value. The Standard bar in the figure represents the standard deviation.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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