[논문]산삼 공생 미생물 Weizmannia ginsengihumi LGHNH의 특징 및 배양물의 항노화 효능

권민정; 이혜진; 이소영; 진무현

doi:10.48022/mbl.2204.04007

산삼 공생 미생물 Weizmannia ginsengihumi LGHNH의 특징 및 배양물의 항노화 효능
Characterization of Weizmannia ginsengihumi LGHNH from Wild-Ginseng and Anti-Aging Effects of Its Cultured Product 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.50 no.3, 2022년, pp.414 - 421

권민정 (LG 생활건강 기술연구원) , 이혜진 (LG 생활건강 기술연구원) , 이소영 (LG 생활건강 기술연구원) , 진무현 (LG 생활건강 기술연구원)

초록
AI-Helper

식물 공생 미생물은 기주 식물과 함께 공생하는 미생물로 생장 촉진, 면역력 증진, 대사체 생성 등의 역할을 수행하며 식물 발달에 영향을 준다. 본 연구를 통해 30년근 산삼에서 분리 동정한 미생물인 W. ginsengihumi LGHNH (KCTC 14462BP)은 식물 생장 촉진 호르몬인 indole-3-acetic acid (IAA)을 1.38 ㎍/ml에서 2.22 ㎍/ml 수준으로 분비함을 확인하였다. 또한 발효 전, 후의 진세노사이드 함량 비교를 통해 진세노사이드 전환능이 있음을 확인하였다. 전환된 저분자 진세노사이드인 Rg2(R), Rg4, Rg6, Rg3(S), Rg3(R), Rk1, Rg5, Rh1(R), Rk3, Rh4 등은 생체 이용률이 높고 다양한 피부 효능을 갖는다고 알려져 있다. 배양물로 제조한 W. ginsengihumi LGHNH (W. ginsengihumi LGHNH Cultured product, WCP)의 항노화 소재로서 가능성을 탐색하기 위해 미토콘드리아의 막전위와 ATP 생합성량을 측정하여 기능 저하 억제 여부를 확인하였다. 노화를 발생시키는 인자인 UVB를 조사한 HaCaT 세포 내 미토콘드리아 막전위 값을 측정한 결과, 미조사군 대비 39.3%로 감소하나 WCP 0.001% (v/v), 0.01% (v/v)에 의해 각각 57.3%, 58.1% 수준까지 회복함을 확인하였다. 또한 미토콘드리아의 ATP 생합성량 측정 결과, UVB 조사에 의해 미조사군 대비 94.3% 수준으로 감소하나 WCP를 0.001% (v/v), 0.01% (v/v) 처리한 군에서 각 각 105.3%, 105.7%로 증가하여 미토콘드리아 기능을 정상으로 회복하는데 도움을 줄 수 있다고 판단된다. 따라서, 본 연구를 통해 확보한 30년근 산삼의 공생 미생물은 항노화 관련 생물 자원으로서 산업적 활용 가능성이 높다.

Abstract ▼ AI-Helper

In this study, we isolated Weizmannia ginsengihumi LGHNH (KCTC 14462BP) from 30-year-old wild Panax ginseng C.A. Meyer and elucidated the characteristics of the isolated bacterium and its industrial potential as an anti-aging material. W. ginsengihumi LGHNH was investigated to produce indole-3-acetic acid (IAA), a plant growth-promoting hormone (1.38 ㎍/ml to 2.22 ㎍/ml). We also confirmed the existence of bioconversion activity via the comparison of the ginsenoside content before and after fermentation. As for the converted minor ginsenoside, Rg2(R), Rg4, Rg6, Rg3(S), Rg3(R), Rk1, Rg5, Rh1(R), Rk3 and Rh4 are known to have high bioavailability and various skin effects. We measured mitochondrial membrane potential and ATP biosynthesis to elucidate W. ginsengihumi LGHNH cultured product (WCP) as an anti-aging material. As a result, the mitochondrial membrane potential in HaCaT cells with UVB decreased to 39.3% compared to the unirradiated group, but was recovered to 57.3% and 58.1% by 0.001% (v/v) and 0.01% (v/v) WCP, respectively. In addition, we measured mitochondrial ATP biosynthesis. It decreased to 94.3% compared to the unirradiated group with UVB, but was recovered to 105.3% and 105.7% by 0.001% (v/v) and 0.01% (v/v) WCP.

주제어

표/그림 (5)

그림 Fig. 1. Concentration of indole-3-acetic acid (IAA) produced by W. ginsengihumi LGHNH. The concentrations of IAA produced were estimated according to a standard curve using pure IAA (Sigma-Aldrich). Each column represents mean ± S.D. (n = 3). There were no significant (NS) difference among samples.
표 Table 1. Contents change of ginsenoside by W. ginsengihumi LGHNH.
그림 Fig. 2. Cell viability of W. ginsengihumi LGHNH cultured product (WCP) in HaCaT cells. (A) Cell viability was measured with CCK-8 assay after treated with various concentrations of WCP (0.001, 0.005, 0.01 and 0.05% (v/v)) for 24 h. (B) Cells were irradiated with 50 mJ/cm² UVB before treatment of WCP. After UVB irradiation, various concentrations of WCP were treated for 24 h. Data represent mean ± S.D. (n = 3). #, p < 0.05 compared to non-treated control and *, p < 0.05 compared to UVB-treated control.
그림 Fig. 3. Effect of W. ginsengihumi LGHNH cultured product (WCP) on UVB induced decrease of mitochondrial membrane potential (Δψm) in HaCaT cells. (A) Decrease and recovery of mitochondrial membrane potential were visualized with JC-1 staining following treatment with UVB and/or of WCP. Cells were irradiated 50 mJ/cm²UVB and treated with WCP as indicated concentration 0.001 and 0.01% (v/v). Then, the cells cultured for 24 h. Images were taken by fluorescent microscopy. (B) Relative Δψm represented with Red/ Green fluorescence ratio. Data represent mean ± S.D. (n = 3). ###, p < 0.001 compared to non-treated control and *, p < 0.05 compared to UVB-irradiated control.
그림 Fig. 4. Effect of W. ginsengihumi LGHNH cultured product (WCP) on UVB induced decrease of ATP synthesis in HaCaT cells. Decrease and recovery of ATP synthesis following treatment with UVB and/or of WCP. Cells were irradiated 50 mJ/cm² UVB and treated with WCP as indicated concentration 0.001 and 0.01 % (v/v). Then, the cells cultured for 24 h, analyzed with CellTiter-Glo® Luminescent Cell Viability Assay. Data represent mean ± S.D. (n = 3). ###, p < 0.001 compared to non-treated control and *, p < 0.05 compared to UVB-irradiated control.

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