[논문]LKB1/AMPK 신호 전달 경로의 활성화로 인한 새싹율무 열수 추출물의 항비만 효과

김민주; 이정훈; 최정원; 박해진; 신미래; 노성수

doi:10.6116/kjh.2021.36.6.39

LKB1/AMPK 신호 전달 경로의 활성화로 인한 새싹율무 열수 추출물의 항비만 효과
Coix lacryma-jobi var. mayuen Stapf Sprout Extract Ameliorates High-Fat Diet-Induced Obesity by Upregulating LKB1/AMPK Signaling 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.36 no.6, 2021년, pp.39 - 46

김민주 (대구한의대학교 한의과대학 본초학교실) , 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) , 최정원 (대구한의대학교 한의과대학 본초학교실) , 박해진 (DHU 바이오융복합시험센터) , 신미래 (대구한의대학교 한의과대학 본초학교실) , 노성수 (대구한의대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives : AMP-activated protein kinase (AMPK) is a key metabolic regulator that reduces lipogenesis. AMPK is mainly activated via phosphorylation of liver kinase B (LKB) 1 under energy stress. Here, we highlighted the anti-obesity effect and underlying mechanism of Coix lacryma-jobi var. mayuen Stapf sprout water extract (CSW) sprout extract in connection with the LKB1/AMPK signaling pathway. Methods : C57BL/6 mice (20~25 g) fed HFD to induce obesity and at the same time administered CSW 100 mg/kg (CSWL; (CSWL; CSW low concentration) or CSW 200 mg/kg (CSWH; CSW high concentration) or Garcinia extract (Garcinia) 200 mg/kg orally for 6 weeks. Body weight and food intake were measured at the same time each day. After 6 weeks of CSW administration, liver tissue and serum were obtained through an autopsy. After the end of the experiment, biochemical analysis (triglycerides (TG), total cholesterol (TC), HDL-cholesterol, and LDL-cholesterol) was performed on the serum. And then, protein levels related to TG and TC synthesis were measured through western blot analysis in liver tissue. Results : As a result, serum TG, TC, and LDL-cholesterol levels were significantly increased in the control group and significantly decreased in the CSW administration group. On the other hand, the HDL-cholesterol level was increased in the CSW-administered group. And as a result of Western blot analysis, CSW significantly increased the phosphorylation of LKB1 & AMPK, and remarkably decreased the expression of factors related to TG and TC synthesis. Conclusions : Our findings suggest that CSW influences the TG and TC synthesis to positively affect HFD-induced obesity in C57BL/6 mice.

주제어

표/그림 (7)

표 Table 1. The Ingredients of 60% High Fat Diet (HFD)
그림 Fig. 1. Initial body weight, final body weight, gain body weight, and feed efficiency ratio in HFD-fed Obese mice. (A) body weight, (B) feed efficiency ratio. Normal ; normal mice, Control；60% HFD-fed Obese mice, Garcinia；60% HFD-fed Obese mice treated with Garcinia 200 mg/kg/day, CSWL ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 100 mg/kg/day, CSWH ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 200 mg/kg body. All data are expressed mean±SD (n=6 mice per group). Significance: ^###p < 0.001 compared vs. Normal group and ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 vs. Control group by LSD test.
표 Table 2. Total Polyphenol and Flavonoid Contents of CSW
표 Table 3. Triglyceride, Total Cholesterol, HDL-cholesterol, and LDL-cholesterol Levels in Serum.
그림 Fig. 2. Expressions of p-LKB1 and p-AMPK in liver tissue. Normal ; normal mice, Control；60% HFD-fed Obese mice, Garcinia；60% HFD-fed Obese mice treated with Garcinia 200 mg/kg/day, CSWL ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 100 mg/kg/day, CSWH ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 200 mg/kg body. All data are expressed mean±SD (n=6 mice per group). Significance: ^###p < 0.001 compared vs. Normal group and ^**p < 0.01, ^***p < 0.001 vs. Control group by LSD test.
그림 Fig. 3. Expressions of SREBP-1 and SREBP-2 in liver tissue. Normal ; normal mice, Control；60% HFD-fed Obese mice, Garcinia；60% HFD-fed Obese mice treated with Garcinia 200 mg/kg/day, CSWL ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 100 mg/kg/day, CSWH ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 200 mg/kg body. All data are expressed mean±SD (n=6 mice per group). Significance: ^###p < 0.001 compared vs. Normal group and ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 vs. Control group by LSD test.
그림 Fig. 4. Expressions of p-ACC, FAS, SCD-1, and HMGCR in liver tissue. Normal ; normal mice, Control；60% HFD-fed Obese mice, Garcinia；60% HFD-fed Obese mice treated with Garcinia 200 mg/kg/day, CSWL ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 100 mg/kg/day, CSWH ; 60% HFD-fed Obese mice treated with Coix lacryma-jobi var. mayuen Stapf sprout 200 mg/kg body. All data are expressed mean±SD (n=6 mice per group). Significance: ^###p < 0.001 compared vs. Normal group and ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 vs. Control group by LSD test.

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