[논문]등수국 잎 추출물 유래 미백 및 항산화 활성 성분

조성미; 김정은; 이남호

doi:10.15230/scsk.2022.48.2.123

등수국 잎 추출물 유래 미백 및 항산화 활성 성분
Whitening and Anti-oxidative Constituents from the Extracts of Hydrangea petiolaris Leaves 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.2, 2022년, pp.123 - 134

조성미 (제주대학교 화학.코스메틱스학과) , 김정은 (제주대학교 화학.코스메틱스학과) , 이남호 (제주대학교 화학.코스메틱스학과)

초록
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본 연구에서는 등수국 잎 추출물의 미백 및 항산화 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. B16F10 melanoma 세포를 이용한 미백 활성 실험 결과, n-hexane (Hex) 분획물이 세포독성 없는 농도에서 멜라닌 생성 및 세포 내 tyrosinase 효소의 활성을 억제시키고 있음을 확인하였다. 또한 Hex 분획물이 tyrosinase 및 TRP-2 단백질의 발현을 감소시켰다. 반면, ethyl acetate (EtOAc) 분획물은 DPPH 및 ABTS⁺ 라디칼 소거 활성이 우수하였고, H₂O₂로 유도된 세포 손상에 대한 세포보호 효과를 나타내었다. Hex 및 EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 8 개의 화합물을 분리하였다; ethyl linoleate (1), ethyl linolenate (2), 1-linoleoyl glycerol (3), 1-linolenoyl glycerol (4), epi-catechin (5), afzelin (6), quercitrin (7), hyperin (8). 분리된 화합물에 대한 항산화 활성 측정 결과, 화합물 5 - 8의 라디칼 소거 활성이 우수함을 확인하였다. 또한 HPLC 분석을 통해 주성분인 quercitrin (7)의 함량을 측정한 결과, 추출물에서 31.3 mg/g, EtOAc 분획물에서 169.8 mg/g이 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 등수국 잎은 미백 및 항산화 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다.

Abstract ▼ AI-Helper

In this study, the whitening and antioxidant effects of the extracts from Hydrangea petiolaris (H. petiolaris) leaves was confirmed, and the chemical structure was identified by separating the active ingredients. In the whitening tests using α-MSH stimulated B16F10 melanoma cells, the n-hexane (Hex) fraction inhibited the cellular melanogenesis and intracellular tyrosinase activities without causing cell toxicity. In addition, the Hex fraction reduced expression of tyrosinase and TRP-2 protein. Upon the anti-oxidative studies by DPPH and ABTS+ radicals, potent radical scavenging activities were observed in the ethyl acetate (EtOAc) fraction. Also, for the cellular protective effects on HaCat keratinocytes damaged by H2O2, the EtOAc fraction indicated protective effects against oxidative stress. Eight phytochemicals were isolated from the extract of H. petiolaris leaves; ethyl linoleate (1), ethyl linolenate (2), 1-linoleoyl glycerol (3), 1-linolenoyl glycerol (4), epi-catechin (5), afzelin (6), quercitrin (7), hyperin (8). Among the isolates, the compounds 5 - 8 showed DPPH and ABTS+ radical scavenging activities. The contents of quercitirin, a major isolated in this extract, determined by HPLC analysis were confirmed to be about 31.3 mg/g for the 70% ethanol extract and 169.8 mg/g for the EtOAc fraction. Based on these results, it was suggested that the extract from H. petiolaris leaves could be potentially applicable as whitening and anti-oxidative ingredients in cosmetic formulations.

주제어

표/그림 (10)

$Figure 1. Effects of extract and solvent fractions from H. petiolaris leaves on melanin contents and cell viability in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or with α-MSH plus fractions from H. petiolaris leaves and melasolv (positive control, 20 μM) for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)$ 그림 Figure 1. Effects of extract and solvent fractions from H. petiolaris leaves on melanin contents and cell viability in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or with α-MSH plus fractions from H. petiolaris leaves and melasolv (positive control, 20 μM) for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
$Figure 2. Effects of n-Hex fraction from H. petiolaris leaves on melanin contents and cell viability in α-MSH induced B16F10 cells. The cells were s timulated with 1 00 n M of α-M SH o nly, or with α-MSH plus n-Hex fraction from H. petiolaris leaves for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)$ 그림 Figure 2. Effects of n-Hex fraction from H. petiolaris leaves on melanin contents and cell viability in α-MSH induced B16F10 cells. The cells were s timulated with 1 00 n M of α-M SH o nly, or with α-MSH plus n-Hex fraction from H. petiolaris leaves for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
$Figure 3. Intracellular tyrosinase activity of n-Hex fraction from H. petiolaris leaves in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or with α-MSH plus n-Hex fraction from H. petiolaris leaves for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)$ 그림 Figure 3. Intracellular tyrosinase activity of n-Hex fraction from H. petiolaris leaves in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or with α-MSH plus n-Hex fraction from H. petiolaris leaves for 72 h. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
$Figure 4. Effects of n-Hex fraction from H. petiolaris leaves on levels of Tyrosinase and TRP-2 in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or w ith α -MSH p lus n-Hex fraction from H. petiolaris leaves for 72 h. The Tyrosinase and TRP-2 protein levels in each sample was normalized to the quantity of β-actin.$ 그림 Figure 4. Effects of n-Hex fraction from H. petiolaris leaves on levels of Tyrosinase and TRP-2 in α-MSH induced B16F10 cells. The cells were stimulated with 100 nM of α-MSH only, or w ith α -MSH p lus n-Hex fraction from H. petiolaris leaves for 72 h. The Tyrosinase and TRP-2 protein levels in each sample was normalized to the quantity of β-actin.
$Figure 5. Total polyphenol contents and total flavonoid contents of extract and solvent fractions from H. petiolaris leaves. The data represent the mean ± SD of triplicate experiments.$ 그림 Figure 5. Total polyphenol contents and total flavonoid contents of extract and solvent fractions from H. petiolaris leaves. The data represent the mean ± SD of triplicate experiments.
$Figure 6. Cell viability of extract and solvent fractions from H. petiolaris leaves. HaCaT cells were treated with different concentration of samples, and then cell toxicity was determined by MTT assay. The data represent the mean ± SD of triplicate experiments.$ 그림 Figure 6. Cell viability of extract and solvent fractions from H. petiolaris leaves. HaCaT cells were treated with different concentration of samples, and then cell toxicity was determined by MTT assay. The data represent the mean ± SD of triplicate experiments.
표 Table 1. SC₅₀ Values of Extract and Solvent Fractions from H. petiolaris Leaves on DPPH and ABTS⁺ Radical Scavenging Activities
그림 Figure 7. (A) Cell viability on HaCaT cells damaged by H₂O₂. (B) Cell protective effects of EtOAc fration from H. petiolaris leaves on HaCaT cells damaged by H₂O₂. HaCaT cells were treated with different concentration of EtOAc fration from H. petiolaris leaves for 24 h after being exposed to oxidative stress. The data represent the mean ± SD of triplicate experiments. (*p < 0.05, **p < 0.01)
그림 Figure 8. Isolated compounds 1 – 8 from H. petiolaris leaves.
$Figure 9. HPLC chromatogram of 70% EtOH extract (A) and EtOAc (B) fraction from H. petiolaris leaves at 279 nm.$ 그림 Figure 9. HPLC chromatogram of 70% EtOH extract (A) and EtOAc (B) fraction from H. petiolaris leaves at 279 nm.

참고문헌 (20)

S. H. Hwang, D. S. Yoo, and J. H. Shim, Whitening effects of banana leaf extract, Kor. J. Soc. Cosmet. Sci., 42(1), 37 (2016).
H. Seo, G. Y. Seo, S. Z. Ko, and Y. H. Park, Ihibitory effects of ethanol extracts from Polygoni multiflori radix and Cynanhi wilfordii radix on melanogenesis in melanoma cells, J. Korean Soc. Food. Sci. Nutr., 40(8), 1086 (2011).
T. H. Kim, J. M. Kim, J. M. Baek, T. W. Kim, D. J. Kim, J. H. Park, and M. Choe, Antioxidant and whitening effects of Agrimonia pilosa Ledeb water extract, Kor. J. Medicinal Crop Sci., 19(3), 177 (2011).
S. N. Park, Effects of natural products on skin cells, J. Soc. Cosmet. Sci. Korea, 25(2), 77 (1999).
R. R. Bartz and C. A. Piantadosi, Clinical review: oxygen as a signaling molecule, Crit Care, 14(5), 234 (2010).

상세보기
M. B. Kim, S. H. Hyu n, J. S. Park, M. A. Kang, Y. H. Ko, and S. B. Lim, Integral antioxidative capacity of extracts by pressurized organic solvent from natural plants in Jeju, J Korean Soc Food Sci Nutr, 37(11), 1491 (2008).
J. H. Park, Master's Thesis Dissertation, Jeju National Univ., Jeju, Korea (2019).
S. M. Jo, J. E. Kim, and N. H. Lee, Anti-inflammatory and anti-bacterial active ingredients derived from the extract of the leaves of Hydrangea petiolaris, J. Soc. Cosmet. Sci. Korea, 46(3), 207 (2020).
T. Gutfinger, Polyphenols in olive oils, J. A m. Oil Chem. Soc., 58(11), 966 (1981).
F. B. Davis, E. Middleton, P. J. Davis, and S. D. Blas, Inhibition by quercetin of thyroid hormone stimulation in vitro of human red blood cell Ca2+-ATPase activity, Cell Calcium, 4(2), 71 (1983).

상세보기
M. S. Blois, Antioxidant determination by the use of a stable free radical, Nature, 181, 1199 (1958).
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, Antixoidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med., 26(9-10), 1231 (1999).
S. H. Park, J. E. Kim, and N. H. Lee, Isolation and evaluation of anti-oxidative constituents from the extract of Ficus erecta var. sieboldii King leaves, J. Soc. Cosmet. Sci. Korea, 42(4), 321 (2016).
S. Huh, Y. S. Kim, E. S. Jung, J. H. Lim, K. S. Jung, M. O. Kim, J. S. Lee, and D. H. Park, Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis, Biol. Pharm. Bull., 33(7), 1242 (2010).

상세보기
S. J. Lee, H. J. Jang, Y. S. Kim, H. M. Oh, S. Y. Lee, K. S. Ju ng, Y. H. Kim, W. S. Lee, S. W. Lee, and M. C. Rho, Inhibitory effects of IL-6-induced STAT3 activation of bio-active compounds derived from Salvia plebeia R.Br., Process Biochemistry, 51, 2222 (2016).

상세보기
J. T. Lee, Master's Thesis Dissertation, Jeju National Univ., Jeju, Korea (2018).
G. H. Xu , I. J. Ryoo, Y. H. Kim, S. J. Choo, and I. D. Yoo, Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis, Arch. Pharm. Res., 32(2), 275 (2009).

원문보기 상세보기
G. Shen, S. R. Oh, B. S. Min, J. Lee, K. S. Ahn, Y.H. Kim, and H. K. Lee, Phytochemical investigation of Tiarella polyphylla, Arch. Pharm., 31(1), 10 (2008).
M. H. Yang and L. Y. Kong, Flavonols and flavonol glycosides from Rhododendron irroratum, Chemistry of Naturla Compounds, 44, 98 (2008).
G. A. Ko and S. Kim Cho, Ethyl linoleate inhibits α-MSH-induced melanogenesis through Akt/GSK3β/β-catenin signal pathway, Korean J. Physiol. Pharmacol., 22(1), 53 (2018).

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