[논문]참당귀(Angelica gigas Nakai)의 체력증진 및 면역조절효과

전용덕; 김수진

doi:10.7732/kjpr.2022.35.4.411

참당귀(Angelica gigas Nakai)의 체력증진 및 면역조절효과
The Regulatory Effect of Angelica gigas Nakai on Immune Enhancement and Cytokine Production in vivo and in vitro 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.35 no.4, 2022년, pp.411 - 416

전용덕 (우석대학교 한약학과) , 김수진 (대구한의대학교 바이오산업융합학부)

초록
AI-Helper

본 연구에서는 다양한 실험모델을 활용하여 참당귀 추출물의 면역조절 효능을 평가하였다. 특히 강제수영부하실험에서 참당귀 추출물의 경구투여는 부동시간의 감소 및 신장기능 지표인 BUN 및 근육의 피로관련 생화학적 지표인 LDH 활성 조절로 체력증진 효과를 나타내는 것으로 확인하였다. T세포를 활용한 참당귀 추출물의 면역조절 효능 평가를 위하여 IL-2, IL-4 및 IFN-𝛾 mRNA 발현 변화에 대한 영향을 측정하였다. 실험결과, 참당귀 추출물은 IL-2, IL-4 및 IFN-𝛾 세포 활성물질의 생성 조절을 통하여 면역조절 효능이 있음을 확인하였다. 본 연구결과를 통해 규명된 결과는 참당귀 추출물의 체력증진 및 면역조절을 위한 건강 기능성 제품개발에 활용 가능성을 제시하였다.

Abstract ▼ AI-Helper

Natural products are important sources for drug development because they have a wide variety of useful biological properties. Angelica gigas Nakai (AGN) has been used as an herbal medicine for treatment of colds, pain, and anemia. The present study was designed to evaluate the regulatory effect of AGN on immune enhancement in vivo and in vitro. To investigate the immune-enhancing effect of AGN, we used forced swimming test (FST) experimental model. Mice were orally administered by AGN or distilled water for 14 days and then immobility time and biological parameters in serum were measured. The results showed that immobility time in AGN treatment group was significantly reduced in comparison with the control group. Plasma levels of blood urea nitrogen and lactate dehydrogenase in AGN group was significantly decreased compared with control group. Additionally, we showed that AGN treatment significantly increased immune-related cytokines such as interleukin (IL)-4, IL-2, and interferon (IFN)-𝛾 levels in Molt-4 cells. Collectively, the findings provide experimental evidence that AGN may be effective in improving immune function.

주제어

표/그림 (5)

표 Table 1. Information of oligonucleotide primers for RT-PCR
그림 Fig. 1. Effect of AGN on forced swimming-induced immobility in mice. AGN (50, 100, 200 mg/kg/day) or saline (control group) were orally administered for 14 days and immobility time was measured after 7 and 14 day's (n=7) administration. Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (*P < 0.05 versus control).
그림 Fig. 2. Effect of AGN on forced swimming-induced biochemical parameters change in mice. AGN (50, 100, 200 mg/kg/day) or saline (control group) were orally administered for 14 days and the levels of BUN, CK, LDH and Glucose in serum were measured by the autoanalyzer. Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (*P < 0.05 versus control).
그림 Fig. 3. Effect of AGN on cell viability in molt-4 cells. Cells (3 × 10⁵ cells/well) were treated with various concentration of AGN (0.25, 0.5, 1, 2 mg/mL) for 24 h and cell viability was measured using the MTT assay. The values are represented as the mean ± S.D. of independent experiments (*P < 0.05 vs. control).
그림 Fig. 4. Effect of AGN on IL-2, IL-4 및 IFN-γ mRNA levels in molt-4 cells. Cells (1 × 10⁶ cells/well) were treated with various concentration of AGN (0.25, 0.5, 1, 2 mg/mL) for 12 h and IL-2, IL-4 및 IFN-γ mRNA levels was measured using qRT-PCR assay. The values are represented as the mean ± S.D. of independent experiments (*P < 0.05 vs. control).

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