[논문]다시마 추출물의 항산화와 염증 조절 효과

최가미; 김은영; 이윤경

doi:10.7318/kjfc/2022.37.5.438

다시마 추출물의 항산화와 염증 조절 효과
Antioxidant and Immunomodulatory Effects of Laminaria japonica Water Extract 원문보기

韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture, v.37 no.5, 2022년, pp.438 - 445

최가미 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) , 김은영 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) , (산동농업대학교 동물영양학과, 중-한 식품유래 기능성 다당류 공동 연구개발센터) , 이윤경 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터)

Abstract ▼ AI-Helper

Laminaria japonica is a type of brown algae widely consumed in Asian countries and contains many essential nutrients and exhibits anti-obesity, antioxidant, and anti-inflammatory effects. In this study, the antioxidant and immunomodulatory effects of a Laminaria japonica water extract (LJE) were investigated using an in vitro model. Mean total polyphenol content of LJE was 2.16±0.11 ㎍ GAE/mg, and LJE dose-dependently inhibited ABTS radical activity but did not scavenge DPPH radicals. In addition, LJE enhanced nitric oxide (NO) production and upregulated the mRNA expressions of proinflammatory cytokines (i.e., tumor necrosis factor-α and interleukin-6) in RAW 264.7 cells. On the other hand, LJE inhibited NO production and downregulated proinflammatory cytokine mRNA levels in endotoxin-stimulated RAW 264.7 cells. Thus, our data show that LJE has moderate antioxidant activity and biphasic immunomodulatory effects on RAW 264.7 cells. In summary, the study indicates that LJE has potential therapeutic use as a novel biphasic immuno-modulator.

주제어

표/그림 (6)

표 Primer sequences for RT-qPCR
그림 ABTS and DPPH radical scavenging activity of LJE. (A) ABTS radical scavenging activity of LJE, (B) DPPH radical scavenging activity of LJE. Statistical analysis was performed using one-way ANOVA. **p<0.01, ***p<0.001, ****p<0.0001 compared with control group (0 μg/mL concentration). Abbreviations: LJE, Laminaria japonica extract; AA, Ascorbic acid.
표 Total polyphenol contents of LJE
그림 Cellular toxicity of LJE in RAW 264.7 cells. Cytotoxicity of LJE on RAW 264.7 cells determined via an MTT assay. The data are presented as the mean±SEM of three independent experiments. Statistical analysis was performed using one-way ANOVA. Asterisks (*) indicate significant differences of p <0.05. Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; LJE, Laminaria japonica extract.
그림 Immunomodulatory effect of LJE on RAW 264.7 cells. RAW 264.7 cells were pretreated with LJE (1 μg/mL) for 24 hrs. (A) Effect of LJE on nitric oxide (NO) production in RAW 264.7 cells. (B) Effect of LJE on Tnf-α, Il-6 and Il-1β mRNA expression in RAW 264.7 cells. (C) Effect of LJE on proinflammatory cytokines TNF-α production in RAW 264.7 cells. The data are presented as the mean±SD of three independent experiments. Statistical analysis was performed using t-test. Asterisks (*) indicate significant differences from control (*p<0.05, ** p<0.01). Abbreviations: LJE, Laminaria japonica extract, NO: nitric oxide, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, IL-1β: interleukin-1β.
그림 Anti-inflammatory effect of LJE on LPS-induced RAW 264.7 cells. RAW 264.7 cells were pretreated with LJE (1 μg/mL) for 6 hrs, then incubated with LPS (100 ng/mL) for another 18 hrs. (A) Effect of LJE on nitric oxide (NO) production in RAW 264.7 cells. (B) Effect of LJE on Tnf-α, Il-6 and Il-1β mRNA expression in RAW 264.7 cells. (C, D) Effect of LJE on proinflammatory cytokines TNF-α and IL-6 production in RAW 264.7 cells. The data are presented as the mean±SD of three independent experiments. Statistical analysis was performed using t-test. Asterisks (*) indicate significant differences from LPS treated group (*p<0.05, **p<0.01, ***p<0.001). Abbreviations: LJE, Laminaria japonica extract, NO: nitric oxide, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, IL-1β: interleukin-1β.

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