[논문]갈랑가 뿌리 추출물의 항산화, 항노화 효과 및 W/O형 에멀젼에서 항산화 효과의 보존성

윤선영; 김봉환; 장영아; 김세기

doi:10.3839/jabc.2023.057

[국내논문] 갈랑가 뿌리 추출물의 항산화, 항노화 효과 및 W/O형 에멀젼에서 항산화 효과의 보존성
Antioxidant and anti-aging effects of Alpinia galanga L. rhizome extracts and preservation of antioxidant effects in W/O type emulsion 원문보기

Journal of applied biological chemistry, v.66, 2023년, pp.424 - 435

윤선영 (Department of Pharmaceutical Engineering, Daegu Catholic University) , 김봉환 (Department of Semiconductor and electronic Engineering, Daegu Catholic University) , 장영아 (Division of Cosmetic Science and Technology, Daegu Haany University) , 김세기 (Department of Pharmaceutical Engineering, Daegu Catholic University)

초록
AI-Helper

본 연구 결과 DPPH 라디칼 소거 활성은 갈랑가 뿌리 70% 에탄올 추출물(AG.E) 100 ㎍/mL 농도에서 81.8%이었으며 ABTS⁺ 라디칼 소거 활성은 AG.E 50 ㎍/mL의 낮은 농도에서 L-Ascorbic acid (AA)와 유사한 99.8%로 확인되었다. 항노화 활성을 측정하기 위하여 collagenase와 elastase 저해 활성을 측정한 결과 둘 모두에서 AG.E는 50 ㎍/mL의 낮은 농도부터 epigallocatechin (EGCG)보다 더 높은 저해 효과를 나타내었다. 특히 500 ㎍/mL 농도에서 EGCG 대비 3배 이상의 저해 효과를 보였다. CCD-986sk 세포내에서 AG.E의 항노화 효과를 검증하기 위해 UVB로 자극한 다양한 실험에서도 우수한 항노화 효과가 얻어졌다. RT-PCR을 통한 유전자 발현 분석 실험에서 COL1A mRNA 발현량은 AG.E 20 ㎍/mL 저농도에서 무첨가 대비 2.90배 증가시키는 결과가 얻어져 항노화관련 우수한 기능성 소재로 개발 가능성이 확인되었다. 제형에 소재의 적용 시 생리활성의 경시적 보전성에 대한 기초 연구로서 AG.E 및 AA등을 안정한 W/O type emulsion에 첨가하여 25 ℃ 항온조에 보관하면서 1일차, 30일차, 60일차에 DPPH와 ABTS+ 라디칼소거 활성을 측정한 결과 모두 경시적으로 항산화 효과가 높은수준 유지됨을 확인하였다.

Abstract ▼ AI-Helper

As a result of this study, DPPH radical scavenging activity was 81.8% at a concentration of 100 ㎍/mL of Alpinia galanga L. rhizome 70% ethanol extract (AG.E), and ABTS+ radical scavenging activity was confirmed to be 99.8%, similar to L-Ascorbic acid (AA), at a low concentration of 50 ㎍/mL AG.E. To measure anti-aging activity, collagenase and elastase inhibitory activities were measured and AG.E showed higher inhibitory effects than epigallocatechin gallate (EGCG) starting at a low concentration of 50 ㎍/mL. In particular, AG.E showed inhibitory effects more than three times that of EGCG at a concentration of 500 ㎍/mL. In order to verify anti-aging effect of AG.E in CCD-986sk cell, good anti-aging effect was obtained in various experiments stimulated with UVB. In a gene expression analysis experiment using RT-PCR, the COL1A mRNA expression level was found to increase 2.90 times compared to no addition at a low concentration of 20 ㎍/mL AG.E, confirming the possibility of developing it as a good functional material related to anti-aging. As a basic study on temporal biological activity preservation ratio of material when applied to formulations, AG.E, and AA were added to a stable W/O type emulsion and stored in a thermostat at 25 ℃ for 60 days. As a result of measuring DPPH and ABTS+ radical scavenging activities on the 1st, 30th, and 60th days, it was confirmed that antioxidant effects are maintained at a high level over time in formulations.

주제어

참고문헌 (45)

Qin Y, Li X, Wu J, Yu K (2022) A management method of chronic？diseases in the elderly based on IoT security environment. Comput Electr？Eng 102: 108188. doi: 10.1016/j.compeleceng.2022.108188

상세보기
Shlisky J, Bloom DE, Beaudreault AR, Tucker KL, Keller HH, FreundLevi Y, Fielding RA, Cheng FW, Jensen GL, Wu D, Meydani SN (2017)？Nutritional Considerations for Healthy Aging and Reduction in AgeRelated Chronic Disease. Adv Nutr 8: 17-26. doi: 10.3945/an.116.013474

상세보기
Kline KA, Bowdish DME (2016) Infection in an aging population.？Current Opinion in Microbiology 29: 63-67. doi: 10.1016/j.mib.2015.11.003

상세보기
Kim HS, Shin KO (2022) A theoretical Study for the Introduction of？Beauty Welfare System in Korea, Aging Society. J Kor Soc Cosmetol？28: 165-171. doi: 10.52660/JKSC.2022.28.1.165

상세보기
Lowe P, Mcbride-Henry K (2012) What factors impact upon the quality？of life of elderly women with chronic illnesses: Three women's？perspectives. Contemporary Nurse 41: 18-27. doi: 10.5172/conu.2012.41.1.18

상세보기
Shin JW, Kwon SH, Choi JY, Na JI, Huh CH, Choi HR, Park KC (2019)？Molecular Mechanisms of Dermal Aging and Antiaging Approaches. Int？J Mol Sci 20: 2126. doi: 10.3390/ijms20092126

상세보기
Pittet JC, Freis O, Smit N, Vazquez-Duchene MD, Perie G, Pauly G？(2014) Evaluation of Elastin/Collagen Content in Human Dermis inVivo by Multiphoton Tomography-Variation with Depth and？Correlation with Aging. Cosmetics 1: 211-221. doi: 10.3390/cosmetics1030211

상세보기
Kim YA, Kim DH, Yu JM, Park CB, Park TS, Park BJ (2017) Antiwrinkle effects of extracts and solvent fractions from Nymphoides peltata？on CCD-986sk. J Appl Biol Chem 60: 357-362. doi: 10.3839/jabc.2017.056

원문보기 상세보기
Park SH, Kim JM, Kim JH, Oh YS, Joo DH, Lee EY, Shin HS, Kim？AR, Lee SL, Park SN (2017) Antioxidative Effects and Component？Analysis of Graviola (Annona muricata) Leaf Extract/Fractions. J Soc？Cosmet Sci Korea 43: 309-320. doi: 10.15230/SCSK.2017.43.4.309

원문보기 상세보기
Lim SB, Kim MU, Lee EH, Kim YJ, Choi EB, Park KI, Kang IK, Choi？YJ (2018) Anti-Oxidant and Inhibitory Activities on Elastase,？Collagenase, Hyaluronidase, and α-Glucosidase of Cedrela sinensis？Fruits. J Korean Soc Food Sci Nutr 47: 1085-1092. doi: 10.3746/jkfn.2018.47.11.1085

상세보기
Lee KE, Son SH, Kang SG (2018) The Coffee Sliver Skin Extracts from？Coffee Beans Exhibited Cosmetic Properties with Antioxiant Activity？and Inhibitory Effects for Elastase, Collagenase and Tyrosinase. J Soc？Cosmet Sci Korea 44: 39-48. doi: 10.15230/SCSK.2018.44.1.39

원문보기 상세보기
Oliveira MM, Ratti BA, Dare RG, Silva SO, Truiti MCT, UedaNakamura T, Auzely-Velty R, Nakamura CV (2019) Dihydrocaffeic？Acid Prevents UVB-Induced Oxidative Stress Leading to the Inhibition？of Apoptosis and MMP-1 Expression via p38 Signaling Pathway.？Oxidative Medicine and Cellular Longevity 2019: 2419096. doi: 10.1155/2019/2419096

상세보기
Na EJ, Jang HH, Kim GR (2016) Review of Recent Studies and？Research Analysis for Anti-oxidant and Anti-aging Materials. Asian J？Beauty Cosmetol 14: 481-491. doi: 10.20402/ajbc.2016.0107
Sonani RR, Rastogi RP, Madamwar D (2015) Antioxidant Potential of？Phycobiliproteins: Role in Anti-Aging Research. Biochem Anal？Biochem 4: 1000172. doi: 10.4172/2161-1009.1000172

상세보기
Masaki H (2010) Role of antioxidants in the skin: Anti-aging effects.？Journal of Dermatological Science 58: 85-90. doi: 10.1016/j.jdermsci.2010.03.003

상세보기
Khairullah AR, Solikhah TI, Ansori ANM, Fadholly A, Ramandinianto？SC, Ansharieta R, Widodo A, Riwu KHP, Putri N, Proboningrat A,？Kusala MKJ, Rendragraha BW, Putra ARS, Anshori A (2020) A Review？of an Important Medicinal Plant: Alpinia galanga (L.) Willd. Sys Rev？Pharm 11: 387-395
Oonmetta-aree J, Suzuki T, Gasaluck P, Eumkeb G (2006) Antimicrobial？properties and action of galangal (Alpinia galanga Linn.) on？Staphylococcus aureus. LWT-Food Science and Technology 39: 1214-1220. doi: 10.1016/j.lwt.2005.06.015

상세보기
Park BY, Lee SK, Jeong IH, Park SK, Lee SB (2018) Insecticidal？activities and repellent effects of methylcinnamate and essential oils from？Alpinia galangal against nymphs and adults of Metcalfa pruinose. J Appl？Biol Chem 61: 291-295. doi: 10.3839/jabc.2018.041

원문보기 상세보기
Hidayat IW, Astuti RT, Kusuma TM (2021) Product Development of？Anti Acne Gel: Combination of White Turmeric Rhizome Ethanol？Extract (Curcuma Mango Val) and Galangal Rhizome Ethanol Extract？(Alpinia Galanga). Urecol Journal Part C: Health Sciences 1: 17-23. doi: 10.53017/ujhs.42
Chouni A, Paul S (2018) A Review on Phytochemical and？Pharmacological Potential of Alpinia galanga. Pharmacogn J 10: 9-15.？doi: 10.5530/pj.2018.1.2

상세보기
Zeng QH, Lu CL, Zhang XW, Jiang JG (2015) Isolation and？identification of ingredients inducing cancer cell death from the seeds of？Alpinia galanga, a Chinese spice. Food Funct 6: 431-443. doi: 10.1039/c4fo00709c

상세보기
Ramanunny AK, Wadhwa S, Singh SK, Kumar B, Gulati M, Kumar A,？Almawash S, Saqr AA, Gowthamarajan K, Dua K, Singh H, Vishwas S,？Khursheed R, Parveen SR, Venkatesan A, Paudel KR, Hansbro PM,？Chellappan DK (2022) Topical non-aqueous nanoemulsion of Alpinia？galanga extract for effective treatment in psoriasis: In vitro and in vivo？evaluation. International Journal of Pharmaceutics 624: 121882. doi: 10.1016/j.ijpharm.2022.121882

상세보기
Gomes A, Costa ALR, Perrechil FA, Cunha RL (2016) Role of the？phases composition on the incorporation of gallic acid in O/W and W/O？emulsions. J Food Eng 168: 205-214. doi: 10.1016/j.jfoodeng.2015.07.041

상세보기
Kim WH, Lee KS, Lee KK (2012) An Experimental Study on the？Property and Stability of W/O emulsion by Various Structures of？Emulsifier. J Soc Cosmet Sci Korea 38: 119-131. doi: 10.15230/SCSK.2012.38.2.119

원문보기 상세보기
Yeon JY, Seo JM, Kim TH, Shin JG (2018) Emulsion Stability of Low？Viscosity W/O emulsion and Application of Inorganic Sunscreen Agents.？Journal of Oil & Applied Science 35: 985-1001. doi: 10.12925/jkocs.2018.35.3.985

원문보기 상세보기
Blois MS (1958) Antioxidant determinations by the use of a stable free？radical. Nature 181: 1199-1200 doi: 10.1038/1811199a0

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C？(1999) Antioxidant activity applying an improved ABTS radical cation？decolorization assay. Free Radic Biol Med 26: 1231-1237. doi: 10.1016/S0891-5849(98)00315-3

상세보기
Cannell RJP, Kellam SJ, Owsianka AM, Walker JM (1988) Results of a？large scale screen of microalgae for the production of protease inhibitors.？Planta medica 54: 10-14. doi: 10.1055/s-2006-962319

상세보기
Wunsch E, Heidrich HG (1963) Zur quantitativen bestimmung der？kollagenase. Hoppe-Seyler's Physiol Chem 333: 149-151. doi: 10.1515/bchm2.1963.333.1.149

상세보기
Huang H, Chen AY, Ye X, Guan R, Rankin GO, Chen YC (2020)？Galangin, a Flavonoid from Lesser Galangal, Induced Apoptosis via？p53-Dependent Pathway in Ovarian Cancer Cells. Molecules 25: 1579.？doi:10.3390/molecules25071579

상세보기
Divakaran SA, Hema PS, Nair MS, Nair CKK (2013) Antioxidant？capacity and radiopro tective properties of the flavonoids galangin and？kaempferide isolated from Alpinia galanga L. (Zin giberaceae) against？radiation induced cellular DNA damage. Inter J Radiat Res 11: 81-89
Iwami M, Shiina T, Hirayama H, Shima T, Takewaki T, Shimizu Y？(2011) Inhibitory effects of zingerone, a pungent component of Zingiber？officinale Roscoe, on colonic motility in rats. J Nat Med 65: 89-94. doi:？10.1007/s11418-010-0463-0

상세보기
Villano D, Fernandez-Pachon MS, Troncoso AM, Garcia-Parrilla MC？(2005) Comparison of antioxidant activity of wine phenolic compounds？and metabolites in vitro. Analytica Chimica Acta 538: 391-398. doi:？10.1016/j.aca.2005.02.016

상세보기
Lee KE, Bharadwaj S, Yadava U, Kang SG (2019) Evaluation of？caffeine as inhibitor against collagenase, elastase and tyrosinase using in？silico and in vitro approach. J Enzyme Inhib Med Chem 34: 927-936.？doi: 10.1080/14756366.2019.1596904

상세보기
Roh E, Kim JE, Kwon JY, Park JS, Bode AM, Dong Z (2014) Molecular？mechanisms of green tea polyphenols with protective effects against skin？photoaging. Crit Rev Food Sci Nutr 57; 1631-1637. doi:10.1080/10408398.2014.1003365

상세보기
Kim E, Han SY, Hwang K, Kim D, Kim EM, Hossain MA, Kim JH,？Cho JY (2019) Antioxidant and Cytoprotective Effects of (-)-？Epigallocatechin-3-(3"-O-methyl) Gallate. Int J Mol Sci 20; 3993. doi:10.3390/ijms20163993

상세보기
Ying QL, Rinehart AR, Simon SR, Cheronis JC (1991) Inhibition of？human leucocyte elastase by ursolic acid. Biochem J 277: 521-526. doi:10.1042/bj2770521

상세보기
Park JG, Kramer BS, Steinberg SM, Carmichael J, Collins JM, Minna？JD, Gazdar AF (1987) Chemosensitivity Testing of Human Colorectal？Carcinoma Cell Lines Using a Tetrazolium-based Colorimetrie Assay.？Cancer Research 47: 5875-5879.
ISO 10993-5:2009 Biological Evaluation of Medical Devices. Part 5？(2009) Tests for In Vitro Cytotoxicity. International Organization for？Standardization, Geneva, Switzerland
Bae I, Kim TG, Kim T, Kim D, Kim DH, Jo J, Lee YJ, Jeong YI (2022)？Phenethyl Isothiocyanate-Conjugated Chitosan Oligosaccharide？Nanophotosensitizers for Photodynamic Treatment of Human Cancer？Cells. Int J Mol Sci 23; 13802. doi: 10.3390/ijms232213802

상세보기
Jang YA, Lee JT (2018) Anti-wrinkle effect of berberine by inhibition of？MMP-2 and MMP-9 activity in fibroblasts. J Appl Biol Chem 61: 9-15.？doi: 10.3839/jabc.2018.002

원문보기 상세보기
Chithra P, Sajithlal GB, Chandrakasan G (1998) Influence of Aloe vera？on collagen characteristics in healing dermal wounds in rats. Mol Cell？Biochem 181: 71-76. doi: 10.1023/a:1006813510959

상세보기
Xue S, Li L (2011) Upregulation of collagen type 1 in aged murine？dermis after transplantation of dermal multipotent cells. Clin Exp？Dermatol 36: 775-781. doi: 10.1111/j.1365-2230.2011.04071.x

상세보기
Bikiaris ND, Koumentakou I, Hatzistamatiou K, Lykidou S, Barmpalexis？P, Nikolaidis N (2023) Preparation and Investigation of the SPF and？Antioxidant Properties of O/W and W/O emulsions Containing Vitamins？A, C and E for Cosmetic Applications. Cosmetics 10: 76. doi: 10.3390/cosmetics10030076

상세보기
Bahtz J, Gunes DZ, Syrbe A, Mosca N, Fischer P, Windhab EJ (2016)？Quantification of Spontaneous W/O Emulsification and its Impact on the？Swelling Kinetics of Multiple W/O/W Emulsions. Langmuir 32: 5787-5795. doi: 10.1021/acs.langmuir.6b00425

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증