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[국내논문] 큐어링 후 저장에 따른 고구마 저장뿌리 단백질체의 비교분석
Comparative proteome profiling in the storage root of sweet potato during curing-mediated wound healing 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.50, 2023년, pp.1 - 10  

신호용 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원)) ,  지창윤 (한국생명공학연구원 식물시스템공학연구센터) ,  김호수 (한국생명공학연구원 식물시스템공학연구센터) ,  정정성 (경상국립대학교 농업생명과학대학 농학과(농업생명과학연구원)) ,  최성환 (경상국립대학교 농업생명과학대학 원예과학부(농업생명과학연구원)) ,  곽상수 (한국생명공학연구원 식물시스템공학연구센터) ,  김윤희 (경상국립대학교 사범대학 생물교육과(농업생명과학연구원)) ,  이증주 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원))

초록
AI-Helper 아이콘AI-Helper

고구마(Ipomoea batatas L. Lam)는 영양소, 가공 식품, 동물 사료 및 색소 재료의 유용한 공급원으로 이용 가능한 경제적으로 중요한 대표적인 뿌리 작물이다. 일반적으로 고구마의 저장 뿌리는 수확 후 저장 기간 동안 다양한 미생물과 질병에 의한 부패에 노출되기 쉽다. 수확 후 큐어링은 저장기간 동안 상처를 치유하고 미생물에 의한 부패를 방지하기 위한 가장 적합한 수단으로 알려져 있다. 본 연구에서는 큐어링과 연관된 분자적 기작에 관여하는 단백질들을 확인하기 위해, 큐어링 처리 후 저장기간 동안 단백질체의 변화를 분석하였다. 33℃ (큐어링) 및 15℃ (대조군)에서 3일 동안 처리하고 8주의 저장 기간이 지난 후 2D 전기영동 분석을 통해 단백질 spot의 변화를 확인한 결과, 31개 단백질 spot의 발현량이 차이 나는 것을 확인하였으며, 이들 중 15개의 단백질 spot을 동정하여 그 특성을 분석하였다. 동정된 단백질 중 alphaamylase (spot 1)는 큐어링 처리구에서만 발현량이 증가하였으며, probable aldo-keto reductase 2-like (spot 3) 및 hypothetical protein CHGG_01724 (spot 4)는 큐어링 및 대조구에서 동시에 발현량이 증가하였으나, sporamin A (spot 10)는 큐어링 및 대조구에서 발현량이 감소하였다. 한편, 대조구에서 enolase (spot 14)는 발현량이 증가하였으나, chain A of actinidin-E-64 complex+ (spot 19), ascorbate peroxidase (spot 22) 및 여러 sporamin 단백질들(spot 20, 21, 23, 24, 27, 29, 30 및 31)은 발현량이 감소하였다. 본 연구의 결과는 고구마 저장 뿌리에서 큐어링 처리와 관련된 단백질의 동정 및 수확 후 저장 기간동안 병 저항성과 관련된 기작에 대한 이해를 높이며, 향후 저온 저장 능력이 향상된 신품종 개발을 위한 후보 유전자의 도출에도 기여할 수 있을 것이다.

Abstract AI-Helper 아이콘AI-Helper

Sweet potato (Ipomoea batatas L. Lam) is an economically important root crop and a valuable source of nutrients, processed foods, animal feeds, and pigment materials. However, during post-harvest storage, storage roots of sweet potatoes are susceptible to decay caused by various microorganisms and d...

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AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

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