[논문]벨벳콩(Mucuna pruriens) 추출물의 항산화 효능 검증

박남완; 한동근; 갈격; 배민준; 김현정; 김세기; 최은영; 안봉전

doi:10.15230/scsk.2023.49.1.9

벨벳콩(Mucuna pruriens) 추출물의 항산화 효능 검증
Verification of Antioxidant Activity from Velvet Bean (Mucuna pruriens) Extracts 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.49 no.1, 2023년, pp.9 - 16

박남완 (대구한의대학교 일반대학원 화장품약리학과) , 한동근 (대구한의대학교 일반대학원 화장품약리학과) , 갈격 (대구한의대학교 일반대학원 화장품약리학과) , 배민준 (대구한의대학교 일반대학원 화장품약리학과) , 김현정 ((주)허니스트) , 김세기 (대구가톨릭대학교 제약공학과) , 최은영 (대구한의대학교 화장품약리학과) , 안봉전 (대구한의대학교 일반대학원 화장품약리학과)

초록
AI-Helper

본 연구에서는 벨벳콩(Mucuna pruriens, M. pruriens) 추출물을 활용하여 천연소재 기반 항산화 기능성 화장품 소재로서의 활용가치를 검증하고자 하였다. 벨벳콩을 물과 70% 에탄올, 70% 아세톤을 용매로 하여 추출, 농축, 동결건조한 다음 각각의 분말화된 벨벳콩 열수 추출물(MW), 벨벳콩 70% ethanol 추출물(ME), 벨벳콩 70% acetone 추출물(MA) 시료를 확보하였다. MW, ME, MA를 활용하여 DPPH 라디칼 소거 활성, ABTS⁺ 라디칼소거 활성, SOD 유사 활성, 환원력, 총 페놀 및 플라보노이드 함량 분석을 통해 항산화 활성을 분석한 결과, 모두 우수한 항산화 효능을 나타내었다. 따라서 벨벳콩 추출물은 항산화 관련 기능성 화장품 천연소재로서의 활용 가능성이 높을 것으로 사료된다.

Abstract ▼ AI-Helper

In this study, we tried to verify the value of the use of Mucuna pruriens (M. pruriens) extract as an antioxidant functional cosmetic material based on natural materials. After extracting, concentrating, and freeze-drying M. pruriens with water, 70% ethanol, and 70% acetone as solvents, samples of powdered hydrothermal extract (MW), 70% ethanol extract (ME), and 70% acetone extract (MA) were prepared. As a result of analyzing antioxidant activity through DPPH radical scavenging activity, ABTS+ radical scavenging activity, SOD-like activity, reduction ability, total phenol and flavonoid content analysis using MW, ME, and MA, all showed excellent antioxidant effects. Therefore, it is believed that M. pruriens extract is highly likely to be used as a natural material for antioxidant-related functional cosmetics.

주제어

표/그림 (7)

그림 Figure 1. The procedure for extraction from Mucuna pruriens (M. pruriens).
그림 Figure 2. Electron donating ability of M. pruriens extracts. MW: M. pruriens extracted with hot-water; ME: M. pruriens extracted with 70% ethanol; MA: M. pruriens extracted with 70% acetone; BHA: butylated hydroxyanisole. Result are means ± SD of triplicate data (^a-mValues with small letters are significantly different at p < 0.05 among various concentrations by Duncan's multiple range test).
그림 Figure 3. ABTS⁺ radical scavenging activity of M. pruriens extracts. MW: M. pruriens extracted with hot-water; ME: M. pruriens extracted with 70% ethanol; MA: M. pruriens extracted with 70% acetone; BHA: butylated hydroxyanisole. Result are means ± SD of triplicate data (^a-gValues with small letters are significantly different at p < 0.05 among various concentrations by Duncan's multiple range test).
그림 Figure 4. Superoxide dismutase like activity of M. pruriens extracts. MW: M. pruriens extracted with hot-water; ME: M. pruriens extracted with 70% ethanol; MA: M. pruriens extracted with 70% acetone; Vit.C: L-ascorbic acid. Result are means ± SD of triplicate data (^a-fValues with small letters are significantly different at p < 0.05 among various concentrations by Duncan's multiple range test).
표 Table 1. The Contents of Total Phenol of M. pruriens Extracts
그림 Figure 5. Reducing power of M. pruriens extracts. MW: M. pruriens extracted with hot-water; ME: M. pruriens extracted with 70% ethanol; MA: M. pruriens extracted with 70% acetone; Vit.C: L-ascorbic acid. Result are means ± SD of triplicate data (^a-iValues with small letters are significantly different at p < 0.05 among various concentrations by Duncan's multiple range test).
표 Table 2. The Contents of Total Flavonoid of M. pruriens Extracts

참고문헌 (32)

J. M. Jeong, Antioxidative and antiallergic effects of？aronia (Aronia melanocarpa), J. Korean Soc. Food. Sci.,？Nutr., 37(9), 1109 (2008).
K. G. Jang, H. C. Oh, E. K. Ko, K. H. Kang, S. E.？Park, M. H. Oh, and Y. C. Kim, Free radical scavengers？from the leaves of Albizzia julibrissin, Kor. J.？Pharmacogn., 33(1), 18 (2002).
W. J. Kim, H. S. Kim, J. Opitz, K. Kabayama, and T. J.？Kim, Effect of cymbidium root extracts on oxidative？stress-induced myoblasts damage, J. Life Sci., 24(9), 1019？(2014).
C. H. Jeong, S. G. Choi, and H. J. Heo, Analysis of？nutritional compositions and antioxidative activities of？Korean commercial blueberry and raspberry, J. Korea？Soc. Food Sci. Nutr., 37(11), 1375 (2008).
J. R. Jang and S. Y. Lim, Effect of onion flesh and peel？on chemical components, antioxidant and anticancer？activities, J. Life Sci., 19(11), 1598 (2009).
H. B. Park, I. G. Kyeong, and J. H. Kang, Antioxidant？and anti-inflammatory effects of silymarin, J. Appl. Biol.？Chem., 65(3), 221 (2022).
M. R. Yi, C. H. Kang, and H. J. Bu, Antioxidant and？anti-inflammatory activity of extracts from kohlrabi？(Brassica Oleracea var. gonglodes), J. Korean Oil Chem.？Soc., 34(2), 189 (2017).
S. S. Nam and K. S. Ko, Antioxidants of apple leaf？extract, J. Korea Appl. Sci. Tech., 37(5), 1116 (2020).
M. J. Kim, C. K. Shim, Y. K. Kim, S. J. Hong, J. H.？Park, E. J. Han, C. S. Huh, Y. H. Ryu, H. J. Jee, and S.？C. Kim, Control effect of coffee ground compost and？velvet bean against root-knot nematode, Meloidogyne？incognita in pumpkin, Korean J. Pestic. Sci., 20(1), 47？(2016).
M. K. Ahmad, A. A. Mahdi, K. K. Shukla, N. Islam, S.？P. Jaiswar, and S. Ahmad, Effect of Mucuna pruriens on？semen profile and biochemical parameters in seminal？plasma of infertile men, Fertil. Steril., 90(3), 627 (2008).

상세보기
L. R. Lampariello, A. Cortelazzo, R. Guerranti, C.？Sticozzi, and G. Valacchi, The magic velvet bean of？Mucuna pruriens, J. Tradit. Complement. Med., 2(4), 331？(2011).
A. Rachsee, N. Chiranthanut, P. Kunnaja, S. Sireeratawong,？P. Khonsung, S. Chansakaow, and A. Panthong, A Mucuna？pruriens (L.) DC. seed extract inhibits lipopolysaccharide-induced inflammatory responses in BV 2 microglial cells,？J. Ethnopharmacol., 267, 113518 (2021).
M. S. Blois, Antioxidant determinations by the use of？stable free radical, Nature, 181, 1199 (1958).
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M.？Yang, and C. Rice-Evans, Antioxidant activity applying？an improved ABTS radical cation decolorization assay,？Free Radical Biol. Med., 26(9-10), 1231 (1999).
S. Marklund and G. Marklund, Involvement of superoxide？anion radical in the oxidation of pyrogallol and a？convenient assay superoxide dismutase, Eur. J. Biochem.,？47(3), 469 (1974).
C. Anesini, G. E. Ferraro, and R. Filip, Total polyphenol？content and antioxidant capacity of commercially available？tea (Camellia sinensis) in argentina, J. Agric. Food？Chem., 56(19), 9225 (2008).

상세보기
X. P. Zhuang, Y. Y. Lu, and G. S. Yang, Extraction and？determination of flavonoid in ginkgo, Chinese. Herbe.？Med., 23, 122 (1992).
M. Oyaizu, Studies of products of browning reactions,？Jpn. J. Nutr. Diet., 44(6), 307 (1986).
J. H. Oh, E. H. Kim, J. L. Kim, Y. I. Moon, Y. H.？Kang, and J. S. Kang, Study on antioxidant potency of？green tea by DPPH method, J. Korean Soc. Food Sci.？Nutr., 33(7), 1079 (2004).
J. P. Kim, Y. S. Yang, J. H. Kim, H. H. Lee, E. S.？Kim, Y. W. Moon, J. Y. Kim, and J. K. Chung,？Chemical properties and DPPH radical scavenging ability？of sword bean (Canavalia gladiata) extract, Korean J.？Food Sci. Technol., 44(4), 441 (2012).
Y. E. Kim, J. W. Yang, C. H. Lee, and E. K. Kwon,？ABTS radical scavenging and anti-tumor effects of？Tricholoma matsutake Sing. (pine mushroom), J. Korean？Soc. Food Sci. Nutr., 38(5), 555 (2009).
M. Y. Shon, S. W. Lee, and S. H. Nam, Antioxidant and？anticancer activities of glycine semen germinatum？fermented with germinated black soybean and some？bacteria, Korean J. Food Preserv., 14(5), 538 (2007).
T. Y. Kim, T. W. Jeon, S. H. Yeo, S. B. Kim, J. S.？Kim, and J. S. Kwak, Antimicrobial, antioxidant and？sod-like activity effect of Jubak extract, Korean J. Food？Nutr., 23(3), 299 (2010).
H. K. Kim, G. M. Na, S. H. Ye, and H. S. Han,？Extraction characteristics and antioxidative activity of？Schiznadra chinensis extracts, Korean J. Food Culture.,？19(5), 484 (2004).
J. Y. Hong, S. R. Shin, H. J. Kong, E. M. Choi, S. C.？Woo, M. H. Lee, and K. M. Yang, Antioxidant activity？of extracts from soybean and small black bean, Korean J. Food Preserv., 21(3), 404 (2014).
J. Y. Cha, H. J. Kim, C. H. Chung, and Y. S. Cho,？Antioxidative activities and contents of polyphenolic？compound of Cudrania tricuspidata, J. Korean Soc. Food？Sci. Nutr., 28(6), 1310 (1999).
M. J. Kim, J. S. Choi, E. J. Song, S. Y. Lee, KBWR.？Kim, S. J. Lee, S. J. Kim, S. Y. Yoon, Y. J. Jeon, and？D. H. Ahn, Effects of heat and pH treatments on？antioxidant properties of Ishige okamurae extract, Korean？J. Food Sci. Technol., 41(1), 50 (2009).
J. K. Hong, B. R. Park, G. S. Jeon, and S. B. Lee,？Extraction of flavonoid components from persimmon leaf,？thistle and new green, Appl. Chem. Eng., 27(3), 276？(2016).
W. Y. Song, and J. H. Choi, Total phenols, flavonoid？contents, and antioxidant activity of Spirodela polyrhiza？extracts, J. Life Sci., 27(2), 180 (2017).
J. B. Lee, H. K. Park, J. S. Lee, and M. G. Kim, Studies？on antioxidant activity, total flavonoids and polyphenols,？and reducing power in Yakju with different ratios of？dandelion root, J. East Asian Soc. Dietary Life, 21(6),？882 (2011).
H. J. Kwon, S. H. Beom, J. A. Hyun, E. B. Kang, H. E.？Park, D. G. Han, E. Y. Choi, B. J. An, Analysis of？antioxidant activity, total phenol content, and flavonoid？content of Abelmoschus manihot flower extracts, Korean？J. Food Preserv., 29(1), 157 (2022).

상세보기
S. H. Nam and M .Y. Kang, Screening of antioxidative？activity of legume species, J. Korean Soc. Agric. Chem.？Biotechol., 46(1), 32 (2003).

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