[논문]갈색거저리 유충에서 추출한 펩타이드의 항산화 특성

김삼웅; 갈상완; 지원재; 방우영; 박소정; 김태완; 방규호

doi:10.5352/jls.2023.33.5.383

[국내논문] 갈색거저리 유충에서 추출한 펩타이드의 항산화 특성
Antioxidant Properties of Peptides Extracted from Tenebrio molitor Larvae 원문보기

생명과학회지 = Journal of life science, v.33 no.5, 2023년, pp.383 - 390

김삼웅 (경상국립대학교 농식품바이오융복합연구원) , 갈상완 (경상국립대학교 제약공학과) , 지원재 (국립생물자원관 생물다양성연구과) , 방우영 (국립생물자원관 생물다양성총괄과) , 박소정 (경상국립대학교 수의학과) , 김태완 (경상국립대학교 산학연협력 선도대학 육성사업단) , 방규호 (경상국립대학교 제약공학과)

초록
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본 연구는 기능성 식품의 개발을 위해 갈색거저리 유충 유래 추출물에서 새로운 생리활성 펩타이드를 확인하는 것이다. 동결건조된 T. molitor 유충에서 다양한 용매로 시간에 따라 추출하였다. T. molitor 유충 추출 결과 물 추출물에서 5시간과 10시간에서 총 단백질과 고형분 함량당 각각 가장 높은 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) 활성을 보였다. 물 추출물을 메탄올 농도에 따라 분획하였을 때, 높은 메탄올 농도는 상등액 중에 고분자량 단백질의 수준을 감소시키는 반면, 상등액의 DPPH 활성 수준을 증가시켜 저분자량 펩타이드가 항산화 활성을 매개하는 것으로 나타났다. 유기용매 분배에서 부탄올을 제외한 유기 용매 분배에서 물층에서 유사한 활성을 보였다. 유기용매분획물을 열처리 후 활성이 28~44% 감소하여 열에 대한 불안정한 성분이 존재하는 것으로 추정되었다. 물 추출물에 프로테아제 K를 첨가하면 DPPH 활성이 10~20% 증가하여 전체 단백질에서 방출되는 펩타이드가 항산화 활성을 부분적으로 증가시켰음을 알 수 있다. 그러므로, T. molitor 유충 추출물은 항산화제로써 기능을 수행할 수 있는 물질이 존재하는 것으로 제의할 수 있다.

Abstract ▼ AI-Helper

The goal of this study was to identify new bioactive peptides in extracts derived from Tenebrio molitor (T. molitor) larvae for the development of functional foods. After extraction from freeze-dried T. molitor larvae with various solvents on time course, the extracts showed the highest 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity at 5 and 10 hr per total protein and solid contents, respectively. When the water extract was fractionated, a high methanol concentration led to a reduced level of high-molecular-weight proteins in the centrifugal supernatant, whereas increased DPPH activity in the supernatants suggests low-molecular-weight peptides may mediate antioxidant activity in the supernatant. Most of the organic solvent partitions, excluding butanol, showed similar activities in the water phases, and the organic solvent partition fraction exhibited a 28~44% decrease in activity following heat treatment, implying that some components in the fraction become unstable in the presence of heat. The addition of proteinase K to the water extract increased DPPH activity by 10~20%, suggesting that peptides, when released from total proteins, partially increase antioxidant activity. Therefore, we suggest that the antioxidants in T. molitor larval extracts make them a potential source of functional animal food.

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표/그림 (5)

그림 Fig. 1. DPPH activities depending on solvent extraction time. (A) IC50 value of DPPH activity at each extract time. (B) Analysis of activity at protein level. (C) Analysis of activity at total solid level. X- and Y-axes of A and B represent extraction time and DPPH IC50 value, respectively. This result was carried out by three replications. ^a-cValuesare significantly different in the same column from each other. ^A-CValuesare significantly different in the same row from each other.
$Fig. 2. Result of methanol fractionation. (A) Result of SDS-PAGE; (B) IC50 values of DPPH at each extraction time. (C) Analysis of activity at protein level. (D) Analysis of activity at total solid level. The water extracts were fractioned after adjusting each methanol concentration. X- and Y-axes of C and D represent methanol concentrations and DPPH IC50 values, respectively. M, molecular marker. Methanol conc; methanol concentrate, S/N; supernatant, PPT: precipitate pellet.$ 그림 Fig. 2. Result of methanol fractionation. (A) Result of SDS-PAGE; (B) IC50 values of DPPH at each extraction time. (C) Analysis of activity at protein level. (D) Analysis of activity at total solid level. The water extracts were fractioned after adjusting each methanol concentration. X- and Y-axes of C and D represent methanol concentrations and DPPH IC50 values, respectively. M, molecular marker. Methanol conc; methanol concentrate, S/N; supernatant, PPT: precipitate pellet.
$Fig. 3. Results of organic solvent partition. (A) Results of SDS-PAGE; (B) IC50 values of DPPH in each organic solvent fraction. (C) Analysis of activity at protein level. (D) Analysis of activity at total solid level. X- and Y-axes of C and D indicate organic solvent type and DPPH IC50 values, respectively. M, molecular marker.$ 그림 Fig. 3. Results of organic solvent partition. (A) Results of SDS-PAGE; (B) IC50 values of DPPH in each organic solvent fraction. (C) Analysis of activity at protein level. (D) Analysis of activity at total solid level. X- and Y-axes of C and D indicate organic solvent type and DPPH IC50 values, respectively. M, molecular marker.
그림 Fig. 4. Change in DPPH activity according to heat treatment. (A) Analysis of activity at protein level. (B) Analysis of activity at total solid level. X- and Y-axes of A and B indicate heat-treated time at 80℃ and DPPH IC50 values, respectively.
그림 Fig. 5. Change in DPPH activity according to proteinase K treatment. (A) Results of SDS-PAGE; (B) IC50 values of DPPH at each treated time. (C) Analysis of activity at protein level. (D) Analysis of activity at total solid level. Each sample was treated for the indicated time at 37℃ with 40 mg/ml Proteinase K. Samples 10-1, 10-2, and 10-3 were treated for 10 hr at 37℃ with 40, 80, and 200 mg/ml Proteinase K, respectively. M, molecular marker.

AI 본문요약
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제안 방법

In this study, bioactive substances in Tenebrio molitor larvae were investigated by separating fractions with antioxidant activity via organic solvent extraction, methanol fractionation, and variable organic solvent partition, followed by analysis of antioxidant activity related to peptides.

참고문헌 (26)

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