[논문]일반메밀과 쓴메밀의 식초 추출물의 알코올 대사 및 숙취개선 효능 평가

김수정; 손황배; 박아현; 이종남; 박수형; 남정환; 김도연; 장동칠; 김율호

doi:10.7732/kjpr.2023.36.5.435

일반메밀과 쓴메밀의 식초 추출물의 알코올 대사 및 숙취개선 효능 평가
Evaluation of the Effects of Hangover-Releasing Agent Containing Vinegar Extract in Common Buckwheat and Tartary Buckwheat on Alcohol Metabolism and Hangover Improvement 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.36 no.5, 2023년, pp.435 - 445

김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) , 손황배 (농촌진흥청 기술협력국 수출농업지원과) , 박아현 ((주)동남의화학연구원) , 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) , 박수형 (농촌진흥청 국립식량과학원 고령지농업연구소) , 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) , 김도연 (농촌진흥청 국립식량과학원 고령지농업연구소) , 장동칠 (농촌진흥청 국립식량과학원 기획조정과) , 김율호 (농촌진흥청 국립식량과학원 재배환경과)

초록
AI-Helper

메밀은 루틴과 콜린을 많이 함유한 기능성 작물로 항산화, 항염증, 항당뇨 등의 효과가 있는 것으로 알려져 있다. 일반메밀과 쓴메밀 식초추출물의 알코올대사와 숙취해소에 대한 효능을 비교 평가하기 위해 혈중 알코올 분해능, 혈액과 간조직의 Alcohol dehydrogenase (ADH) 및 Acetaldehyde dehydrogenase (ALDH) 효소 측정, 간 조직의 Superoxide Dismutase (SOD)와 Catalase 농도를 분석하였다. 루틴 함량은 쓴메밀 종실에서 일반메밀 종실보다 55배 높았다. 콜린 함량은 쓴메밀 식초 추출물에서 약간 높게 나타났다. 알코올 분해능은 대조군에 비해 쓴메밀군에서 36.0%의 알코올 농도 감소 효과를 보여 유의한 알코올 분해 효과가 인정되었다. 혈중 아세트알데하이드 농도는 대조군에서는 시간이 경과할수록 증가하였으나, 일반메밀군은 120분, 쓴메밀군은 90분에 최고치 도달 후 감소하여 숙취 개선 효능이 확인되었다. 또한, 혈액과 간 조직의 ADH와 ALDH 효소 활성은 대조군에 비해 메밀처리군에서 증가하는 경향을 보여 숙취해소 효과가 모두 인정되었으며, 일반메밀군보다 쓴메밀군에서 더 우수하였다. 간 조직의 SOD와 Catalase 활성도 일반메밀보다 쓴메밀군에서 각각23%, 37% 고도로 유의미하게 높았다. 이상의 결과를 요약하면, 일반메밀과 쓴메밀 식초추출물은 모두 숙취해소에 효과가 있었으며, 특히 루틴 함량이 높은 쓴메밀 '황금미소'는 일반메밀보다 숙취해소 소재로 개발하는데 활용 가능성이 높을 것으로 판단되었다.

Abstract ▼ AI-Helper

The aim of this study was to explore the effects of vinegar extract from seed of common buckwheat (Fagopyrum esculentum Moench) and seed of tartary buckwheat (F. tataricum Gaertner) on acute ethanol-induced hangover in Sprague-Dawley rats. Vinegar extract from buckwheat is rich choline, quercetin and its glycoside, rutin known as flavonoid antioxidants. The test extract containing buckwheat was proven to alleviate hangovers through a significant reduction in the concentration of alcohol and acetaldehyde in the context of an alcohol-induced hangover model. Hepatic alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were significantly higher in buckwheat vinegar-treated rats than in ethanol-treated rats. Moreover, tartary buckwheat vinegar upregulated antioxidant enzyme such as superoxide dismutase and Catalase activities in liver tissues. These results suggest that buckwheat vinegar extract could alleviate ethanol-induced hangover symptoms by elevating activities related to hepatic ethanol-metabolizing enzymes against ethanol induced metabolites, and in particular, tartary buckwheat should be further developed to be a novel anti-hangover material.

주제어

표/그림 (8)

그림 Fig. 1. Scheme for buckwheat vinegar treatment of hangover mice in experiment. Sprague Dawley (SD) rats were divided into 4 groups: NOR, CON, SCB and STB. NOR, Normal mice fed with vinegar extract group; CON, Alcohol induced hangover mice fed with vinegar extract group; SCB, Alcohol induced hangover mice fed with seed of common buckwheat (Fagopyrum esculentum Moench) with vinegar extract group; STB, Alcohol induced hangover mice fed with seed of tartary buckwheat (Fagopyrum tataricum Gaertn.) with vinegar extract group.
표 Table 1. Tissue weight of liver, spleen, kidney in EtOH-induced mice fed with vinegar extract from common and tartary buckwheat seed
표 Table 2. Rutin, quercetin and choline contents in fresh seed and vinegar extract from common buckwheat and tartary buckwheat
그림 Fig. 2. UPLC analysis of rutin and quercetin contents in fresh seed and vinegar extract from common buckwheat and tartary buckwheat. A, fresh seed of common buckwheat; B, fresh seed of tartary buckwheat; C, vinegar extract of common buckwheat; D, vinegar extract of tartary buckwheat. All chromatograms passed through spectrofluorometric detector at 259 nm. Retention time of all chromatograms were as follows: (1), Rutin (9.022 min); and (2) quercetin (18.039 min).
그림 Fig. 3. HPLC-MS chromatogram of choline from the fresh seed and vinegar extract from common buckwheat and tartary buckwheat. A, fresh seed of common buckwheat; B, fresh seed of tartary buckwheat, C, vinegar extract of common buckwheat; D, vinegar extract of tartary buckwheat. Retention time of all chromatograms were as follows: (1), choline (4.155 min); and (2) Choline-d9 (4.159 min).
그림 Fig. 4. Changes in blood alcohol (A), Area under curve (AUC) (B) and acetaldehyde (C) level in alcohol-induced hangover mice model fed with vinegar extract from common and tartary buckwheat seed. NOR (●), Normal mice group with vinegar extract; CON (■), Alcohol-induced hangover mice group with vinegar extract; SCB (▲), Alcohol induced hangover mice fed with seed of common buckwheat with vinegar extract; STB (▼), seed of tartary buckwheat treated group. Values are expressed Mean±SE on groups of five experiments. Data shown are the mean±SD (n=5). The variables in four material including two buckwheat materials expressed *significant at p < 0.05 vs NOR.
그림 Fig. 5. Changes in Alcohol dehydrogenase ADH activity of serum and liver (A and B), Aldehyde dehydrogenase ALDH activity of serum and liver (C and D) in alcohol-induced hangover mice model fed with vinegar extract from common and tartary buckwheat seed. NOR, Normal mice group; CON, Alcohol-induced hangover mice group; SCB, seed of common buckwheat treated group; STB, seed of tartary buckwheat treated group. Values are expressed Mean±SD on groups of five experiments. Data shown are the mean±SD (n=5). The variables in four material including two buckwheat materials expressed *significant at p < 0.05 vs NOR, # significant at p < 0.05 vs CON.
그림 Fig. 6. Changes in Superoxide dismutase (SOD) and Catalase level of liver (A and B) level in alcohol-induced hangover mice model fed with vinegar extract from common and tartary buckwheat seed. NOR, Normal mice group; CON, Alcohol-induced hangover mice group; SCB, seed of common buckwheat treated group; STB, seed of tartary buckwheat treated group. Values are expressed Mean±SD on groups of five experiments. Data shown are the mean±SD (n=5). The variables in four material including two buckwheat materials expressed *significant at p < 0.05 vs NOR, ^# significant at p < 0.05 vs CON.

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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