[논문]Dextran Sodium Sulfate 유발 마우스 대장염에서 Lactiplantibacillus plantarum JSA22 쌀 발효물의 효과

유지송; 선다현; 최현석; 김민섭; 유명환; 진종식; 김동근; 최혜선; 박정호; 김용성; 이문영

doi:10.9799/ksfan.2024.37.1.001

[국내논문] Dextran Sodium Sulfate 유발 마우스 대장염에서 Lactiplantibacillus plantarum JSA22 쌀 발효물의 효과
Effect of Lactiplantibacillus plantarum JSA22-fermented Rice Drinks against Dextran Sodium Sulfate-induced Colitis in Mice

한국식품영양학회지 = The Korean journal of food and nutrition, v.37 no.1, 2024년, pp.1 - 8

유지송 (원광대학교 의과대학 생리학교실) , 선다현 (원광대학교 의과대학 생리학교실) , 최현석 (원광대학교 의과대학 생리학교실) , 김민섭 (원광대학교 의과대학 생리학교실) , 유명환 (원광대학교 의과대학 생리학교실) , 진종식 (전북대학교 한약자원학과) , 김동근 (전북대학교 한약자원학과) , 최혜선 (농촌진흥청) , 박정호 (강북삼성병원 소화기내과) , 김용성 (주식회사 것앤드푸드헬스케어) , 이문영 (원광대학교 의과대학 생리학교실)

Abstract ▼ AI-Helper

This study investigated the effect of Lactiplantibacillus plantarum JSA22-fermented rice drinks on dextran sodium sulfate (DSS)-induced colitis in mice. Twenty-four mice were randomly assigned; No colitis (Con), colitis with tap water (DSS-only), colitis with unfermented rice (DSS-UFR), and colitis with fermented rice (DSS-FR). After inducing colitis with 2% DSS for 5 days, they were given Tap water, UFR drink, or FR drink for an additional 6 days. The DSS-FR group had significantly lower Disease Activity Index (DAI) scores compared to the DSS-only group, but no significant difference with the DSS-UFR group. Colon length was reduced in the DSS-only group. The DSS-only group had significantly higher IL-6 mRNA levels compared to the Con group, while the DSS-FR groups showed significantly lower IL-6 mRNA levels compared to the DSS-only group. These results suggest that rice drinks fermented with Lactiplantibacillus Plantarum JSA22 ameliorate the severity of DSS-colitis, by potentially reducing proinflammatory cytokines.

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표/그림 (5)

표 Table 1. Primer sequences used for real-time quantitative PCR
그림 Fig. 1. Effects of fermented rice products on disease activity in DSS-induced colitis mice. (A) Food intake, (B) Body weight, (C) Disease activity index (DAI) score ^#Denotes significant difference between the DSS-only and DSS-FR group (^#p<0.05, n=6).
그림 Fig. 2. Effects of fermented rice products on luminol response test in DSS-induced colitis mice. (A) The representative image of luminol response test. (B) The statistical analysis of luminol response test. ^#Denotes significant difference between the DSS-only and DSS-FR group (^###p<0.001, n=6).
그림 Fig. 3. Effect of fermented rice products on length of colon in DSS-induced colitis mouse. (A) The representative image of colon length. (B) The statistical analysis of the colon length. *Denotes significant difference between control and the DSS-only groups (p<0.05, n=6).
그림 Fig. 4. Effect of fermented rice products on the colonic inflammatory marker expression in DSS-induced colitis mice. Relative mRNA expression levels of IL-6 (A) and TNF-α (B) in the colon tissues between groups. *Denotes significant difference between control and the DSS-only groups (p<0.05). ^#Denotes significant difference between DSS-only and DSS-FR group (p<0.05, Con, n=6; DSS-only, n=5; DSS-UFR, n=6, DSS-FR, n=6).

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