[논문]Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity

Kim, Young-Il; Casel, Mark Anthony B.; Kim, Se-Mi; Kim, Seong-Gyu; Park, Su-Jin; Kim, Eun-Ha; Jeong, Hye Won; Poo, Haryoung; Choi, Young Ki

doi:10.1007/s12275-020-0335-6

[국내논문] Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity 원문보기

The journal of microbiology, v.58 no.10, 2020년, pp.886 - 891

Kim, Young-Il (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Casel, Mark Anthony B. (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Kim, Se-Mi (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Kim, Seong-Gyu (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Park, Su-Jin (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Kim, Eun-Ha (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Jeong, Hye Won (College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644 Republic of Korea) , Poo, Haryoung (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon, 34141 Republic of Korea) , Choi, Young Ki (College)

Abstract ▼ AI-Helper

Various treatments and agents had been reported to inactivate RNA viruses. Of these, thermal inactivation is generally considered an effective and cheap method of sample preparation for downstream assays. The purpose of this study is to establish a safe inactivation method for SARS-CoV-2 without compromising the amount of amplifiable viral genome necessary for clinical diagnoses. In this study, we demonstrate the infectivity and genomic stability of SARSCoV- 2 by thermal inactivation at both 56°C and 65°C. The results substantiate that viable SARS-CoV-2 is readily inactivated when incubated at 56°C for 30 min or at 65°C for 10 min. qRT-PCR of specimens heat-inactivated at 56°C for 30 min or 65°C for 15 min revealed similar genomic RNA stability compared with non-heat inactivated specimens. Further, we demonstrate that 30 min of thermal inactivation at 56°C could inactivate viable viruses from clinical COVID-19 specimens without attenuating the qRT-PCR diagnostic sensitivity. Heat treatment of clinical specimens from COVID-19 patients at 56°C for 30 min or 65°C for 15 min could be a useful method for the inactivation of a highly contagious agent, SARS-CoV-2. Use of this method would reduce the potential for secondary infections in BSL2 conditions during diagnostic procedures. Importantly, infectious virus can be inactivated in clinical specimens without compromising the sensitivity of the diagnostic RT-PCR assay.

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[국내논문] Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity 원문보기

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[국내논문] Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity 원문보기

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