[논문]An ultrasensitive aptamer-antibody sandwich cortisol sensor for the noninvasive monitoring of stress state

Huang, Ziyu; Chen, Hao; Ye, Huarong; Chen, Zixuan; Jaffrezic-Renault, Nicole; Guo, Zhenzhong

doi:10.1016/j.bios.2021.113451

An ultrasensitive aptamer-antibody sandwich cortisol sensor for the noninvasive monitoring of stress state

Biosensors & bioelectronics, v.190, 2021년, pp.113451 -

Huang, Ziyu (Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology) , Chen, Hao (Department of Anaesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) , Ye, Huarong (China Resources and Wisco General Hospital) , Chen, Zixuan (Department of Clinical Medicine, Medical College, Wuhan University of Science and Technology) , Jaffrezic-Renault, Nicole (University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280) , Guo, Zhenzhong (Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology)

Abstract ▼ AI-Helper

Abstract Cortisol is a major glucocorticoid that can affect physiological activities in the human body. Besides, it is also a biomarker that can reflect the stress state of the body. Therefore, in order to monitor stress states in a sensitive and non-invasive manner, an ultra-sensitive aptamer-antibody sandwich sensor modified with multi-walled carbon nanotubes, ordered mesoporous carbon CMK-3, and silver nanoparticles (MWCNTs/CMK-3/AgNPs) was proposed for non-invasive detection of cortisol in human saliva. The MWCNTs/CMK-3/AgNPs nanocomposite was fixed on the surface of the glassy carbon electrodes (GCEs) as the material for the first round of signal amplification, and secondary signal amplification was realized by conjugating cortisol antibodies with gold nanoparticles (AuNPs). Finally, the aptamer-antibody sandwich pattern was used to specifically recognize and bind cortisol. The concentration response range for this aptamer-antibody sandwich sensor is 0.1 pg/mL-10 ng/mL, and the limit of detection (LOD) is 0.09 pg/mL. So far, the LOD of this sensor has been relatively low, showing its good sensitivity, selectivity, stability, and reproducibility. Furthermore, it has been successfully applied to detect cortisol in saliva samples to compare the stress states of postgraduates and undergraduates. Highlights The aptamer-antibody sandwich pattern was used to capture cortisol cooperatively. Up to date, the limit of detection was as low as 0.09 pg/mL. Cortisol was measured in students' saliva samples to monitor the stress status.

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An ultrasensitive aptamer-antibody sandwich cortisol sensor for the noninvasive monitoring of stress state

Abstract ▼ AI-Helper

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