보고서 정보
주관연구기관 |
경북대학교 KyungPook National University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2015-02 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
과제관리전문기관 |
농촌진흥청 Rural Development Administration |
등록번호 |
TRKO201500010613 |
DB 구축일자 |
2015-07-11
|
초록
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Ⅳ. 연구개발결과
1. 제 1 세부과제
① 가시박(Sicyos angulatus L.) 종자의 상추 발아억제 효과와 물질 동정
- 상추종자에 가시박 종자 추출물 처리를 통한 발아억제능 확인
- Silica gel column chromatography 및 TLC, 분액추출 등을 이용한 물질 정제
- GC/MS를 이용하여 가시박 추출물의 억제 활성 물질 2-Linoleoyl Glycerol 동정
② 가시박(Sicyos angulatus L.) 종자 추출물 처리를 통한 상추 생육 억제
- 가시박 종
Ⅳ. 연구개발결과
1. 제 1 세부과제
① 가시박(Sicyos angulatus L.) 종자의 상추 발아억제 효과와 물질 동정
- 상추종자에 가시박 종자 추출물 처리를 통한 발아억제능 확인
- Silica gel column chromatography 및 TLC, 분액추출 등을 이용한 물질 정제
- GC/MS를 이용하여 가시박 추출물의 억제 활성 물질 2-Linoleoyl Glycerol 동정
② 가시박(Sicyos angulatus L.) 종자 추출물 처리를 통한 상추 생육 억제
- 가시박 종자 조추출물 처리를 통한 상추 생육 억제 활성 확인
- LC-MS/MS를 통한 단백질 (Chloropyll a/b binding protein, oxygen-evolving enhancer protein 1) 동정
- GC/MS와 HPLC를 이용한 식물호르몬 gibberellin(GA), abscisic acid(ABA), salicylic acid(SA), jasmonic acid(JA) 정량 분석
- Spectrophotometer를 통한 엽록소 a/b 및 카로티노이드 정량 분석
③ 식물생장억제 근권세균 선발을 위한 종자 발아 검정 및 상추 생육조사
- 근권토양으로부터 식물생장억제 세균 분리 및 상추의 생육억제 활성 확인
- 분리된 균들 중 가장 효과가 좋았던 I-3의 16S rDNA 염기서열 분석을 통해 세균 동정 결과 Enterobacter sp. 97% 상동성을 보여 Enterobacter sp. I-3이라 명명
2. 제 2 세부과제
④ 생장조절 기능성 평가용 추출물 제조 및 천연물 확보
- 한약소재로 사용되는 식물들을 추출하여 89종과 가시박 종자 추출물 1종을 추출물로 제조하여 후보군 설정
- 식물 뿌리 생장과 관련이 있는 것으로 알려진 페놀성 화합물을 중심으로 정제된 천연물 118종을 확보하여 작물 발아 및 유근생장 억제능을 평가
⑤ 쌈채류 생장조절 기능성 천연추출물의 쌈배추 발아 및 유근생장조절능 평가
- 발아테스트 결과 발아억제기능성 추출물은 박하와 계피의 주정추출물에서 확인됨
- 발아 억제기능성이 확인된 추출물의 발아억제기능 향상 여부를 확인하기 위하여 극성 분배를 통한 물질정제를 실시하였고, hexane, dichloromethane, water 용액으로 구분하여 정제
- 갈근 주정추출물, 강황 주정추출물, 결명자 주정추출물, 가시박 hexane 추출물, 감초 주정추출물, 꾸지뽕나무 주정추출물은 유근생장을 촉진하는 것으로 확인
⑥ 쌈채류생장조절기능성화합물의쌈배추발아및유근생장조절능평가
- 쌈배추에대한발아촉진및억제기능성검증을실시함
- 시험기준농도에서 50%이상 발아억제율을 나타낸 물질에 대하여 IC50값을 측정한 결과 salicylaldehyde가 81mg/L로 가장 강한 활성을 나타냄
- 확보된 정제 천연물질을 활용하여 쌈배추의 유근생장 촉진 및 억제기능성을 검증하였고, 2,3-Dihydroxybenzaldehyde 및 4-hydroxy-3-nitrobenzaldehyde는 10mg/L 수준에서 유근생장을 매우 효율적으로 억제하는 것이 확인
⑦ 쌈채류 생장조절 기능성 물질의 작물 생장조절능 포트시험평가
- 정제 천연물질중 유근생장억제능이 확인된 12종의 유기화학물질 (2OH-5N, 3N-BenA-4OH, BenA-234TME, BenA-23DOH, BenA-245TOH, CinA, CinA-2ME , CinA-5ME, Cin-Al, Cin-M, Ger, SalA에 대한 토양처리 후 지상부 생육평가를 실시
- 시험결과 125 mg/L 이상 처리한 처리구에서 BenA-23DOH, BenA-234TME, CinA-2ME, CinA-5ME가 우수한 지상부 생장억제능을 갖는 것으로 확인
- 개똥쑥 및 계피 추출물은 상추 생장억제 기능성을 나타내지 않았고, 계피 처리구는 지상부 생장이 촉진된 결과를 나타내었으며, 최대엽장 및 최대엽폭등 전체적 생장량이 증가하는 경향을 나타냄
Abstract
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Bio-herbicides from plant extract are effective environmental friendly method to prevent the weed growth. Although, herbal extracts have been screened for inhibiting seed germination and root development of weeds, there were a few reports documented to control invasive weeds. The objective of this r
Bio-herbicides from plant extract are effective environmental friendly method to prevent the weed growth. Although, herbal extracts have been screened for inhibiting seed germination and root development of weeds, there were a few reports documented to control invasive weeds. The objective of this research was to develop bio-herbicides against weed growth by using seed/plant extracts of Artemisia annua, Cinnamomum cassia, Mentha arvensis and Bur-cucumber. In the present study, ninety one herbal extracts were tested on a weed, Brassica campestris to know their plant growth inhibition activity. The alcohol extracts (0.1%, w/v) of A. annua, C. cassia and M. arvensis inhibited 30% of B. campestris seed germination and, while A. annua and C. cassia extracts showed 70% inhibition of radical growth. The partially purified extracts of A. annua and C. cassia with dichloromethane and hexane showed stronger radical growth inhibition on B. campestris than crude alcohol extracts. The further purified diethyl ether extract (45 mg/L) of A. annua inhibited the radical growth of weed at 50%, it was higher than diethyl ether extract of C. cassia. In addition, eighty two pure natural compounds were identified in A. annua extract by using GC-MS. Amoung these, thirty compounds were benzaldehydes, which potently retarded the root growth (> 70%) on B. campestris. The predominant quantity (100 mg/L) of 2-hydroxylated benzaldehydes, 2,3-dihydroxybenzaldehyde, 2,4,5-trihydroxybenzaldehyde and salicylaldehyde significantly inhibited seed germination and root growth of B. campestris.
To determine the inhibitory mechanism of herbal extract on weed growth, the bur-cucumber seed extract was applied on a model plant, lettuce. Bur-cucumber seeds were ground with water and three different concentrations of seed extract (5 %, 10 % and 20 %) were prepared and applied to lettuce plants. The lower rate of plant height, number of leaves, leaf length, leaf width and leaf area was found in lettuce exposed with all bur-cucumber seed extract than their controls. A significant reduction of lettuce biomass was observed in 20 % bur-cucumber seed extract treated plants due to the presence of higher amount of phenolic content in extract. The mechanism of plant growth inhibition was assayed in proteins variation of lettuce by two-dimensional electrophoresis (2-DE) and LC-MS/MS method. In total, 57 protein spots were detected in 20 % bur-cucumber seed extract treated and control plants. Among these, 39 proteins were down-regulated and 18 proteins were up-regulated in 20 % bur-cucumber seed extract exposed plants, when compared to their controls. The presence of low intensity of chlorophyll a/b binding protein and oxygen-evolving enhancer protein 1 in bur-cucumber seed extract exposed plants reduced the photosynthetic pigments synthesis might be a reason to stunted plant growth. Indeed, the plant growth stimulating hormone, gibberellin pathway was inhibited and synthesis of stress hormones such as abscisic acid, jasmonic acid and salicylic acid were triggered in lettuce by the effect of bur-cucumber seed extract. The uptake of essential nutrients, Ca, Fe, Mg, K, S and Mo were deficient and toxic ions, Cu, Zn and Na were higher in bur-cucumber seed extract treated plants. The results of this study suggest that the application of A. annua extract to soil can prevent the B. campestris growth by producing benzaldehydes, and bur-cucumber seed extract can be act as an effective bio-herbicide to weed control by inhibiting photosynthesis machinery components and, regulating phyto-hormones and nutritional elements.
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