보고서 정보
주관연구기관 |
국립원예특작과학원 National Institute of Horticultural and Herbal Science |
연구책임자 |
박혜성
|
참여연구자 |
이찬중
,
이은지
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2021-02 |
과제시작연도 |
2020 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO202100010118 |
과제고유번호 |
1395065101 |
사업명 |
농축산물수출확대장애요인해소기술개발(R&D) |
DB 구축일자 |
2021-10-09
|
키워드 |
수출용버섯.장기저장.재배환경.상품성.안전성.Mushrooms for export.Long-term storage.Cultivation environment.Marketability.Safety.
|
초록
▼
○ 느타리버섯 저장성 향상을 위한 배지내 질소함량 및 최적 생육환경 설정: 질소함량-1.8%, 생육온도-12℃, 생육습도-75%
○ 식품첨가물 9종 중 lactic acid, DL-malic acid,calcium hydroxide 등 3종과 버섯에서 분리한 유산균 106개 균주 중 Leuconosotoc lactis IWM2 균주를 팽이버섯 중 L. monocytogenes 제어물질로 선발
○ 큰느타리 품질 및 저장성 증대를 위한 배지질소함량은 2.1~2.4%, 생육온도는 수확2일전부터 수확 시까지 12℃, 생육상대습도
○ 느타리버섯 저장성 향상을 위한 배지내 질소함량 및 최적 생육환경 설정: 질소함량-1.8%, 생육온도-12℃, 생육습도-75%
○ 식품첨가물 9종 중 lactic acid, DL-malic acid,calcium hydroxide 등 3종과 버섯에서 분리한 유산균 106개 균주 중 Leuconosotoc lactis IWM2 균주를 팽이버섯 중 L. monocytogenes 제어물질로 선발
○ 큰느타리 품질 및 저장성 증대를 위한 배지질소함량은 2.1~2.4%, 생육온도는 수확2일전부터 수확 시까지 12℃, 생육상대습도는 발이유도기 90%, 자실체 신장기 85~90%, 수확2일전부터 80~85%로 하였을때 저온저장 시 60일간 가능
○ 팽이버섯 배지 내 질소함량 1.8%, 억제기 온도 4℃, 생육습도 80% 유지시 저장기간 연장
○ 느티만가닥버섯 배지내 질소 함량 0.8%, 생육온도 13℃, 상대습도 80%로 조절 할 때 신선도 유지 기간 14일 연장
(출처 : 요약서 4p)
Abstract
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□ Purpose&Contents
○ The unit production amount of domestic mushrooms has been on the constant rise, thanks to automated production facilities and mass-production system. The domestic prices of mushrooms are so low that their productivity falls. Therefore, it is necessary to conduct the base rese
□ Purpose&Contents
○ The unit production amount of domestic mushrooms has been on the constant rise, thanks to automated production facilities and mass-production system. The domestic prices of mushrooms are so low that their productivity falls. Therefore, it is necessary to conduct the base research for stable export. As one of staple crops, mushrooms account for 700 billion KRW interms of annual production. Since the FTA, it has been necessary for farmers to raise excellent agricultural products in order to secure competition, expand exports and to make differentiation from competing countries. With the export expansion of mushrooms to the US and European regions, it has been more necessary to develop the management technology after harvest in order to maintain quality in local markets even after the long ship-based transportation. The cultivation environment and quality specification of the mushrooms for export are different depending on farms. In addition, a claim for quality change is made due to long storage. For these reasons, in order for stable export, it is essential to conduct the base research for developing the standard cultivation technology to product mushrooms with a certain level of quality and the technology to store mushrooms long, and for cultivating fresh and safe mushrooms through the prevention of harmful microorganisms.
○ In order to produce oyster mushrooms (Pleurotus ostreatus) with high quality and storage, the purpose is to evaluate the quality and storage of mushrooms according to media combination and environmental conditions, and thereby to develop the technology to keep an optimal cultivationenvironment.
○ To investigate the level of hazardous microorganisms in mushrooms during distribution and to develop control technology for reduce food born pathogens in mushrooms.
○ This experiment was conducted to extend the freshness maintenance period through environmental control such as medium nitrogen content, growth temperature and humidity, and the results were applied to export farms.
□ Results
○ The mycelial growth and density of oyster mushrooms were analyzed according to the nitrogen content of their sawdust medium. As a result, the mycelial growth was not different highly according to the nitrogen content, and the culture completion period was mostly 35 days. There was no large difference according to varieties. The mycelial density was alsonot distinct difference according to the nitrogen content, and allshowed the high mycelial density. In terms of the finally selected growth conditions, the storage period increased by 10 days from 30 days to 40 days in the conditions where the nitrogen content was 1.8%, the growth temperature was 12℃, and the relative humidity was 75%.
○ UV, LED treatment alone was not effective, but exposure to 405nm LED for 3h and treatment with 3% organic acid(lactic acid, malic acid) for 30 min reduce L. monocytogenes in enoki mushrooms by > 4.0 log CFU/g. In addition, the combination of 3% organic acid, 0.1% nisin and 40kHz ultrasound reduced L.monocytogenes in enoki mushrooms by > 5.0 log CFU/g.
○ The nitrogen content of the medium to increase the quality and storage of king oyster mushroom is 2.1~2.4%, the growth temperature is 12℃ from 2 days before harvest to the time ofharvest, the relative humidity of growth is 90% during the induction period, 85~90% of the growth period of fruiting bodies, from 2 days before harvest It was 80-85% until harvest. In the case of storing the fruiting oysters harvested under the above conditions at a low temperature of 1~2℃. Freshness was maintained for 6 to 8 days when stored at room temperature at 20 to 25℃ after low temperature storage at 1~2℃ for 60 days.
○ Setting of total nitrogen content in the medium for cultivation of winter mushroom
○ Establishment of winter mushroom cultivation method for improve storage
○ When the nitrogen content in the medium of beech mushroom (Hypsizygus marmoreus) was 0.8%, its yield increased 37%. When the growth temperature during a harvest period was 13℃, its sellable freshness was maintained up to 63 days. The storage period of the mushroom lengthened 7 days longer than the mushrooms that grew the conventional culture temperature 15℃. When the nitrogen content in the medium of beech mushroom was set to 0.8%, its growth temperature wasto 13℃, and its relative humidity was to 80%, its freshness period was able to be extended 14 days.
□ Expected Contribution
○ The manual of the cultivation environment and after-harvest-management for each variety of mushrooms for export was written, and was used as an educational material in a field. Based on the result of the farm field test on the moisture content percentage in the medium, etc., improvements were made.
Through the field test, the cultivation technology was applied to a field site.
Enhancing safety of exported mushrooms
○ The production environment and post-harvest management manual for king oyster mushroom for export used as educational material in the farm, and on-site demonstration of farmhouses such as controlling moisture content inthe medium
○ Applying cultivation technology on-site by supplementing the results and promoting additional field verification tests
(출처 : SUMMARY 7p)
목차 Contents
- 표지 ... 1제 출 문 ... 2보고서 요약서 ... 3국 문 요 약 문 ... 5Summary ... 7목차 ... 10제 1 장 연구 개발 과제의 개요 ... 11 제1절 연구 개발 목적 ... 11 제2절 연구 개발의 필요성 ... 11 제3절 연구 개발 범위 ... 13제 2 장 연구 수행 내용 및 결과 ... 15 제1절 수출용느타리버섯재배환경조절을통한저장성증대및품질향상연구 ... 15 제2절 유통중 유해미생물 제어를 위한 친환경 기술 개발 ... 27 제3절 큰느타리버섯 재배환경 조절을 통한 저장성 증대 및 품질 향상 연구 ... 65 제4절 팽이버섯 재배환경 조절을 통한 저장성 증대 및 품질향상 연구 ... 95 제5절 느티만가닥버섯 재배환경 조절을 통한 저장성 증대 및 품질향상 연구 ... 107제 3 장 목표달성도 및 관련분야 기여도 ... 127 제1절 목표대비 달성도 ... 127 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 128제 4 장 연구 결과의 활용 계획 ... 129제 5 장 연구 개발 결과의 보안 등급 ... 130제 6 장 연구시설·장비종합정보시스템에등록한 연구시설·장비현황 ... 131제 7 장 연구개발과제의 대표적 연구실적 ... 132제 8 장 기타사항 ... 133제 9 장 참고문헌 ... 134끝페이지 ... 139
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