Enzymes and non-enzymatic antioxidants are involved in defense of oxygen free radical intermediates in all aerobic eukaryotic cells. The non-enzymatic antioxidants and antioxidant enzyme from the extracts of Solanum nigrum known to be anticancer medicinal plant were examined in order to utilize the ...
Enzymes and non-enzymatic antioxidants are involved in defense of oxygen free radical intermediates in all aerobic eukaryotic cells. The non-enzymatic antioxidants and antioxidant enzyme from the extracts of Solanum nigrum known to be anticancer medicinal plant were examined in order to utilize the discovery in natural products as cancer chemopreventive agents. The DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavening activity on plant parts of Solanum nigrum was the highest in root, with stem, whole plant, seed, leaf and flower, at higher activities respectively. The Inhibition activity of xanthine/ xanthine oxidase was the highest in root, with stem, whole plant and seed, at higher activities respectively. but leaf, flower and whole plant were not detection. In extraction methods, the DPPH free radical scavenging activity by circulating extraction with 80% MeOH showed higher than that by shaking extraction with 80% MeOH. And Circulating extraction with 80% MeOH Inhibition activity of xanthine/ xanthine oxidase was high, but shaking extraction with 80% MeOH was not detection. The DPPH activity of L6 fraction by LH-20 column chromatography showed about 6.7 times higher than that of ethyl acetate fraction. and Inhibition activity of xanthine oxidase was also higher than that of ethyl acetate fraction. These were identified as phenolic compounds such as 2,6-methano-3-benzazocin-11-ol, 2[1H]-pyridinethione and 2-hydroxy-5-methyl- benzaldehyde. Peroxidase (POD) and superoxide dismutase (SOD) activities of stem and root were higher than that of other plant parts and those of plant parts according to growing stage were the highest in 60 days after seeding. The numbers of isozyme pattern of POD and SOD showed 10 bands and 5 bands, respectively, especially, 8 bands of POD and 2 bands of SOD showed a difference according to plant parts. Lunasin was searched from seeds of korean medicinal plants, used seed as drug, by coomassie blue staining and western blot Among them, we just found lunasin from Solanum nigrum in mature seeds of korean medicinal plants western blot. To isolate and purify lunasin from Solanum nigrum, we used ion exchange column chromatography with 0.7M NaCl phosphate buffer, so lunasin was confirmed. In addition, we observed the contents of lunasin according to after flowering and confirmed to appear the lunasin after on 30 days flowering. We confirmed that lunasin is complexed protein maturing seeds. Lunasin in Solanum nigrum showed exert inhibitoin effect on proliferation in HL-60 cells.
Enzymes and non-enzymatic antioxidants are involved in defense of oxygen free radical intermediates in all aerobic eukaryotic cells. The non-enzymatic antioxidants and antioxidant enzyme from the extracts of Solanum nigrum known to be anticancer medicinal plant were examined in order to utilize the discovery in natural products as cancer chemopreventive agents. The DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavening activity on plant parts of Solanum nigrum was the highest in root, with stem, whole plant, seed, leaf and flower, at higher activities respectively. The Inhibition activity of xanthine/ xanthine oxidase was the highest in root, with stem, whole plant and seed, at higher activities respectively. but leaf, flower and whole plant were not detection. In extraction methods, the DPPH free radical scavenging activity by circulating extraction with 80% MeOH showed higher than that by shaking extraction with 80% MeOH. And Circulating extraction with 80% MeOH Inhibition activity of xanthine/ xanthine oxidase was high, but shaking extraction with 80% MeOH was not detection. The DPPH activity of L6 fraction by LH-20 column chromatography showed about 6.7 times higher than that of ethyl acetate fraction. and Inhibition activity of xanthine oxidase was also higher than that of ethyl acetate fraction. These were identified as phenolic compounds such as 2,6-methano-3-benzazocin-11-ol, 2[1H]-pyridinethione and 2-hydroxy-5-methyl- benzaldehyde. Peroxidase (POD) and superoxide dismutase (SOD) activities of stem and root were higher than that of other plant parts and those of plant parts according to growing stage were the highest in 60 days after seeding. The numbers of isozyme pattern of POD and SOD showed 10 bands and 5 bands, respectively, especially, 8 bands of POD and 2 bands of SOD showed a difference according to plant parts. Lunasin was searched from seeds of korean medicinal plants, used seed as drug, by coomassie blue staining and western blot Among them, we just found lunasin from Solanum nigrum in mature seeds of korean medicinal plants western blot. To isolate and purify lunasin from Solanum nigrum, we used ion exchange column chromatography with 0.7M NaCl phosphate buffer, so lunasin was confirmed. In addition, we observed the contents of lunasin according to after flowering and confirmed to appear the lunasin after on 30 days flowering. We confirmed that lunasin is complexed protein maturing seeds. Lunasin in Solanum nigrum showed exert inhibitoin effect on proliferation in HL-60 cells.
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#까마중 Solanum nigrum 추출물 항산화제 항암단백질
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