[학위논문]야콘(Smallanthus sonchifolius) 잎 추출물의 항산화 및 인체위암세포주(AGS) Effect of Extracts from Yacon (Smallanthus sonchifolius) Leaves on Antioxidant Activity and Proliferation Inhibition of AGS Cell Lines원문보기
본 연구는 건조 방법에 따른 야콘 잎의 70% 에탄올 추출물을 이용하여 야콘 잎의 항산화 활성과 인체위암세포주 (AGS)에 대한 증식 억제 효과를 확인하였다. 야콘 잎의 항산화 활성을 비교하기 위하여 총 폴리페놀과 플라보노이드 함량을 측정하고, DPPH와 ...
본 연구는 건조 방법에 따른 야콘 잎의 70% 에탄올 추출물을 이용하여 야콘 잎의 항산화 활성과 인체위암세포주 (AGS)에 대한 증식 억제 효과를 확인하였다. 야콘 잎의 항산화 활성을 비교하기 위하여 총 폴리페놀과 플라보노이드 함량을 측정하고, DPPH와 ABTS 라디칼 소거활성을 분석하였다. MTT assay를 통하여 항암 활성을 분석하였고, 세포의 형태 변화와 세포주기를 분석하여 야콘 잎이 생리활성에 미치는 영향을 검토하였다. 항산화 실험 결과, 야콘 잎의 DPPH 라디칼 소거능은 100 µg/mL에서 열풍건조 야콘 잎 26.9%, 동결건조 야콘 잎 54.2% 효과를 나타내었다. ABTS 라디칼 소거능에서는 열풍건조 야콘 잎 44.8%, 동결건조 야콘 잎 81.3%를 나타내어 열풍건조에 비하여 동결건조 야콘 잎에서 더 높은 소거능을 확인하였다. AGS 세포에 야콘 잎 추출물을 농도(25, 50, 100, 200, 400 µg/mL), 시간(24시간, 48시간)에 따라 항암 활성을 확인하였다. 야콘 잎 추출물의 농도와 시간이 증가할수록 암세포를 증식시키는 억제 효과가 높아졌다. 야콘 잎 추출물의 최대 농도인 400 µg/mL, 48시간에서 AGS 세포는 열풍건조 69.6%, 동결건조 75.1%의 위암 증식 억제 효과를 보였다. AGS 세포에 대한 형태 변화 관찰에서도 농도, 시간이 증가할수록 세포의 수가 감소하였으며, 세포의 크기와 형태가 변화되었다. 항암 활성의 대표적인 특징 중 하나인 암세포의 세포주기를 분석한 결과, 야콘 잎 추출물의 최대 농도인 400 µg/mL, 48시간에서 G2/M기(phase)가 49.4%로 증가하여 농도와 시간에 따라 증식 억제 효과가 증가하는 결과를 보였다. 이러한 결과를 바탕으로, 건조 방법에 따라 동결건조 야콘 잎 추출물이 높은 항산화 활성을 보여주어 열풍건조보다 동결건조가 효율성이 높을 것으로 판단된다. 또한 야콘 잎은 AGS 세포에 대하여 증식 억제 효과를 높게 가지는 것으로 확인되어 만성질환을 예방하고, 위암을 예방할 수 있는 건강기능식품 소재로서 이용될 가치가 충분할 것으로 사료된다.
본 연구는 건조 방법에 따른 야콘 잎의 70% 에탄올 추출물을 이용하여 야콘 잎의 항산화 활성과 인체위암세포주 (AGS)에 대한 증식 억제 효과를 확인하였다. 야콘 잎의 항산화 활성을 비교하기 위하여 총 폴리페놀과 플라보노이드 함량을 측정하고, DPPH와 ABTS 라디칼 소거활성을 분석하였다. MTT assay를 통하여 항암 활성을 분석하였고, 세포의 형태 변화와 세포주기를 분석하여 야콘 잎이 생리활성에 미치는 영향을 검토하였다. 항산화 실험 결과, 야콘 잎의 DPPH 라디칼 소거능은 100 µg/mL에서 열풍건조 야콘 잎 26.9%, 동결건조 야콘 잎 54.2% 효과를 나타내었다. ABTS 라디칼 소거능에서는 열풍건조 야콘 잎 44.8%, 동결건조 야콘 잎 81.3%를 나타내어 열풍건조에 비하여 동결건조 야콘 잎에서 더 높은 소거능을 확인하였다. AGS 세포에 야콘 잎 추출물을 농도(25, 50, 100, 200, 400 µg/mL), 시간(24시간, 48시간)에 따라 항암 활성을 확인하였다. 야콘 잎 추출물의 농도와 시간이 증가할수록 암세포를 증식시키는 억제 효과가 높아졌다. 야콘 잎 추출물의 최대 농도인 400 µg/mL, 48시간에서 AGS 세포는 열풍건조 69.6%, 동결건조 75.1%의 위암 증식 억제 효과를 보였다. AGS 세포에 대한 형태 변화 관찰에서도 농도, 시간이 증가할수록 세포의 수가 감소하였으며, 세포의 크기와 형태가 변화되었다. 항암 활성의 대표적인 특징 중 하나인 암세포의 세포주기를 분석한 결과, 야콘 잎 추출물의 최대 농도인 400 µg/mL, 48시간에서 G2/M기(phase)가 49.4%로 증가하여 농도와 시간에 따라 증식 억제 효과가 증가하는 결과를 보였다. 이러한 결과를 바탕으로, 건조 방법에 따라 동결건조 야콘 잎 추출물이 높은 항산화 활성을 보여주어 열풍건조보다 동결건조가 효율성이 높을 것으로 판단된다. 또한 야콘 잎은 AGS 세포에 대하여 증식 억제 효과를 높게 가지는 것으로 확인되어 만성질환을 예방하고, 위암을 예방할 수 있는 건강기능식품 소재로서 이용될 가치가 충분할 것으로 사료된다.
This research was conducted to verify the antioxidation of yacon leaf and inhibition effect on the proliferation of human gastric adenocarcinoma cell (AGS) using yacon leaf ethanol extract which used a different drying method. The total polyphenol and flavonoid content according to the hot-air and f...
This research was conducted to verify the antioxidation of yacon leaf and inhibition effect on the proliferation of human gastric adenocarcinoma cell (AGS) using yacon leaf ethanol extract which used a different drying method. The total polyphenol and flavonoid content according to the hot-air and freeze drying methods of yacon leaf were measured. Also, the effect of different drying methods on the biological activity of yacon leaf was compared and reviewed by analyzing the antioxidant activity with DPPH and ABTS radical scavenging activities and by confirming anticancer activity against the AGS cell of yacon leaf ethanol extract through MTT assay and analyzing cell shape change and cell cycle. The DPPH radical scavenging activities of yacon leaf in hot-air drying and freeze drying were 54.2% and 26.9% respectively at 100 µg/mL; the effect was 44.8% and 81.3% in ABTS radical scavenging, proving that freeze drying yacon leaf had a higher scavenging activity than hot-air drying. In the anticancer activity through MTT assay, yacon leaf extract that applied a different drying method on AGS cell was processed in 25, 50, 100, 200, and 400 µg/mL concentration and the proliferation inhibition activity during 24 and 48 hours was observed. The result demonstrated that inhibition activity of cancer cell proliferation increased in a dose dependent manner of concentration and hours. For 48 hours treatment of 400 µg/mL, the inhibition activity of AGS cell proliferation of hot-air dried yacon leaf extract was 69.6% and freeze dried one was 75.1%, and anticancer activity according to drying method showed a similar tendency. In the observation of AGS cell shape change, the number of cells decreased by increasing the concentration and hours and the shape of cells changed as well. Lastly, the analysis of cancer cell cycle, the most typical characteristics of anticancer activity, demonstrated that G2/M phase increased to 49.4% for 48 hours in 400 µg/mL, showing that the increment was dependent on concentration and hours. These results indicate that yacon leaf extract has not only antioxidant activity but also inhibition effect of AGS cell proliferation, so it is considered useful to prevent chronic diseases and as a health functional food ingredient for preventing cancer. In addition, freeze dried yacon leaf has much higher antioxidant activity than hot-air dried one as demonstrated through DPPH and ABTS radical scavenging, and thus freeze drying is an efficient method for maintaining the biological acrivity.
This research was conducted to verify the antioxidation of yacon leaf and inhibition effect on the proliferation of human gastric adenocarcinoma cell (AGS) using yacon leaf ethanol extract which used a different drying method. The total polyphenol and flavonoid content according to the hot-air and freeze drying methods of yacon leaf were measured. Also, the effect of different drying methods on the biological activity of yacon leaf was compared and reviewed by analyzing the antioxidant activity with DPPH and ABTS radical scavenging activities and by confirming anticancer activity against the AGS cell of yacon leaf ethanol extract through MTT assay and analyzing cell shape change and cell cycle. The DPPH radical scavenging activities of yacon leaf in hot-air drying and freeze drying were 54.2% and 26.9% respectively at 100 µg/mL; the effect was 44.8% and 81.3% in ABTS radical scavenging, proving that freeze drying yacon leaf had a higher scavenging activity than hot-air drying. In the anticancer activity through MTT assay, yacon leaf extract that applied a different drying method on AGS cell was processed in 25, 50, 100, 200, and 400 µg/mL concentration and the proliferation inhibition activity during 24 and 48 hours was observed. The result demonstrated that inhibition activity of cancer cell proliferation increased in a dose dependent manner of concentration and hours. For 48 hours treatment of 400 µg/mL, the inhibition activity of AGS cell proliferation of hot-air dried yacon leaf extract was 69.6% and freeze dried one was 75.1%, and anticancer activity according to drying method showed a similar tendency. In the observation of AGS cell shape change, the number of cells decreased by increasing the concentration and hours and the shape of cells changed as well. Lastly, the analysis of cancer cell cycle, the most typical characteristics of anticancer activity, demonstrated that G2/M phase increased to 49.4% for 48 hours in 400 µg/mL, showing that the increment was dependent on concentration and hours. These results indicate that yacon leaf extract has not only antioxidant activity but also inhibition effect of AGS cell proliferation, so it is considered useful to prevent chronic diseases and as a health functional food ingredient for preventing cancer. In addition, freeze dried yacon leaf has much higher antioxidant activity than hot-air dried one as demonstrated through DPPH and ABTS radical scavenging, and thus freeze drying is an efficient method for maintaining the biological acrivity.
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