The purpose of this study was to examine anti-oxidant effect of Solanum Nigrum fruit extract. The MTT experiment led to having confirmed cytotoxicity through testing polyphenol, flavonoid and DPPH after extracting ethanol. As a result of having figured out the anti-oxidant effect of Solanum Nigrum L...
The purpose of this study was to examine anti-oxidant effect of Solanum Nigrum fruit extract. The MTT experiment led to having confirmed cytotoxicity through testing polyphenol, flavonoid and DPPH after extracting ethanol. As a result of having figured out the anti-oxidant effect of Solanum Nigrum L. after making extract through using the distilled water, 70%EtOH% and 100%EtOH, the content of Polyphenol was indicated to be the highest with 14.62±0.07mg/g at 70%EtOH%. A difference in the content of Flavonoid was not shown greatly. Also, as for DPPH radical scavenging, the inhibitory effect of radical scavenging in 67.84±0.81% was shown at 10000ppm of the extract concentration. In consequence of measuring cytotoxicity, the cell survival rate appeared to be high in all extracts, thereby having been indicated to have no cytotoxicity. It measured pH in cosmetics, evaluated the stability of cosmetics, and carried out a long-term storage test through making the scalp essence of not containing Solanum Nigrum Fruit Extract, the scalp cosmetics in an essence type with the addition of 10% in Solanum Nigrum Fruit Extract, the hair treatment in a lotion type of not containing Solanum Nigrum Fruit Extract, and the hair treatment cosmetics in a lotion type with the addition of 10% in Solanum Nigrum Fruit Extract. In terms of pH variation in scalp essence, pH showed 5.83-4.98. The hair treatment expressed 5.24-5.08. There were no coagulation, precipitation, crystal precipitation, a color change, and phase separation in the long-term storage test. To examine hair care effects of Solanum Nigrum Fruit Extract, the physical and morphological characteristics were gauged by damaging each specimen with dyeing, by making it be divided on the basis of application phase(non-treatment, pre-treatment of Solanum Nigrum L., the addition to Solanum Nigrum L. perm agent No. 1(10%), the intermediate treatment of Solanum Nigrum L., general treatment, the Solanum Nigrum L. treatment(10%), and then by operating the digital permanent wave. In the measurement of wave efficiency, Pb with the pre-treatment of Solanum Nigrum L. stood at 13.76cm with the wave formation rate at 31.2%. Pf with the application of winding phase in Solanum Nigrum L. treatment came to 14.4cm with the wave formation rate at 28%. Thus, it was indicated to be higher than the untreated hair Pa in 16.72cm with the wave formation rate at 16.4%. As a result of having observed hair cuticle, a case of PC, which added Solanum Nigrum L. to perm agent, and a case of Pd, which applied Solanum Nigrum L. to the neutralizing stage in the intermediate treatment, were confirmed to have a protective effect of cuticle in Solanum Nigrum L. because of being regular in an interval of cuticles, of being smooth, and of having no bird-caging and exfoliation. In consequence of measuring tensile strength and extension, the average value of tensile strength in Pa with non-treatment of Solanum Nigrum L. appeared to be 125.72. But the tensile strength in Pb with pre-treatment of Solanum Nigrum L. amounted to 147.05, thereby having been able to know the hair care effect because 16.80% growth rate was shown in comparison with Pa with non-treatment. Still, the extension rate appeared to have a significant difference even though the extension in hair with the application of Solanum Nigrum L. got slightly higher than the extension in Pa with non-treatment of Solanum Nigrum L. As a result of gauging alkali solubility, the alkali solubility in Pa with non-treatment was indicated to be 23.99%. On the other hand, Pb with pre-treatment of Solanum Nigrum L. accounted for 16.42%, thereby having been indicated to be lower than Pa with non-treatment. Pd with the application of the neutralizing stage in the intermediate treatment of Solanum Nigrum L. appeared to be low with 13.19%. Hence, Solanum Nigrum L. was identified to have possessed a hair-damage protection effect. It selected 30 test subjects, measured a scalp condition before and after the experiment, and examined the effect of improving scalp in Solanum Nigrum Fruit Extract while carrying out scalp massage side by side through visiting for 4 weeks with once a week, using 1mL of Solanum Nigrum scalp essence in scalp per day. As a result of looking into the clinical experiment, it was confirmed to have the effect on relieving inflammation in the sensitive scalp, the dandruff scalp, and the scalp with a hair-loss progressive type. The implementation of subjective evaluation on test subjects led to having figured out the scalp improvement effect and applicability in scalp essence of Solanum Nigrum Fruit Extract as natural cosmetics. In the subjective evaluation of test subjects, the Solanum Nigrum Fruit Extract was clarified to have an adequate impact on improving adults' scalp condition and promoting satisfaction with scalp care. Accordingly, in consequence of this research, pH(3.97) and anti-oxidant substance in Solanum Nigrum Fruit Extract were identified to have an effect on improving scalp and to have an effective influence upon satisfaction with scalp care because of efficiently preventing hair damage against chemical treatment given the thermal permanent procedure in the damaged hair and of having anti-inflammatory and antibacterial action. Hence, Solanum Nigrum Fruit Extract is prospected to likely have feasibility as a natural substance.
The purpose of this study was to examine anti-oxidant effect of Solanum Nigrum fruit extract. The MTT experiment led to having confirmed cytotoxicity through testing polyphenol, flavonoid and DPPH after extracting ethanol. As a result of having figured out the anti-oxidant effect of Solanum Nigrum L. after making extract through using the distilled water, 70%EtOH% and 100%EtOH, the content of Polyphenol was indicated to be the highest with 14.62±0.07mg/g at 70%EtOH%. A difference in the content of Flavonoid was not shown greatly. Also, as for DPPH radical scavenging, the inhibitory effect of radical scavenging in 67.84±0.81% was shown at 10000ppm of the extract concentration. In consequence of measuring cytotoxicity, the cell survival rate appeared to be high in all extracts, thereby having been indicated to have no cytotoxicity. It measured pH in cosmetics, evaluated the stability of cosmetics, and carried out a long-term storage test through making the scalp essence of not containing Solanum Nigrum Fruit Extract, the scalp cosmetics in an essence type with the addition of 10% in Solanum Nigrum Fruit Extract, the hair treatment in a lotion type of not containing Solanum Nigrum Fruit Extract, and the hair treatment cosmetics in a lotion type with the addition of 10% in Solanum Nigrum Fruit Extract. In terms of pH variation in scalp essence, pH showed 5.83-4.98. The hair treatment expressed 5.24-5.08. There were no coagulation, precipitation, crystal precipitation, a color change, and phase separation in the long-term storage test. To examine hair care effects of Solanum Nigrum Fruit Extract, the physical and morphological characteristics were gauged by damaging each specimen with dyeing, by making it be divided on the basis of application phase(non-treatment, pre-treatment of Solanum Nigrum L., the addition to Solanum Nigrum L. perm agent No. 1(10%), the intermediate treatment of Solanum Nigrum L., general treatment, the Solanum Nigrum L. treatment(10%), and then by operating the digital permanent wave. In the measurement of wave efficiency, Pb with the pre-treatment of Solanum Nigrum L. stood at 13.76cm with the wave formation rate at 31.2%. Pf with the application of winding phase in Solanum Nigrum L. treatment came to 14.4cm with the wave formation rate at 28%. Thus, it was indicated to be higher than the untreated hair Pa in 16.72cm with the wave formation rate at 16.4%. As a result of having observed hair cuticle, a case of PC, which added Solanum Nigrum L. to perm agent, and a case of Pd, which applied Solanum Nigrum L. to the neutralizing stage in the intermediate treatment, were confirmed to have a protective effect of cuticle in Solanum Nigrum L. because of being regular in an interval of cuticles, of being smooth, and of having no bird-caging and exfoliation. In consequence of measuring tensile strength and extension, the average value of tensile strength in Pa with non-treatment of Solanum Nigrum L. appeared to be 125.72. But the tensile strength in Pb with pre-treatment of Solanum Nigrum L. amounted to 147.05, thereby having been able to know the hair care effect because 16.80% growth rate was shown in comparison with Pa with non-treatment. Still, the extension rate appeared to have a significant difference even though the extension in hair with the application of Solanum Nigrum L. got slightly higher than the extension in Pa with non-treatment of Solanum Nigrum L. As a result of gauging alkali solubility, the alkali solubility in Pa with non-treatment was indicated to be 23.99%. On the other hand, Pb with pre-treatment of Solanum Nigrum L. accounted for 16.42%, thereby having been indicated to be lower than Pa with non-treatment. Pd with the application of the neutralizing stage in the intermediate treatment of Solanum Nigrum L. appeared to be low with 13.19%. Hence, Solanum Nigrum L. was identified to have possessed a hair-damage protection effect. It selected 30 test subjects, measured a scalp condition before and after the experiment, and examined the effect of improving scalp in Solanum Nigrum Fruit Extract while carrying out scalp massage side by side through visiting for 4 weeks with once a week, using 1mL of Solanum Nigrum scalp essence in scalp per day. As a result of looking into the clinical experiment, it was confirmed to have the effect on relieving inflammation in the sensitive scalp, the dandruff scalp, and the scalp with a hair-loss progressive type. The implementation of subjective evaluation on test subjects led to having figured out the scalp improvement effect and applicability in scalp essence of Solanum Nigrum Fruit Extract as natural cosmetics. In the subjective evaluation of test subjects, the Solanum Nigrum Fruit Extract was clarified to have an adequate impact on improving adults' scalp condition and promoting satisfaction with scalp care. Accordingly, in consequence of this research, pH(3.97) and anti-oxidant substance in Solanum Nigrum Fruit Extract were identified to have an effect on improving scalp and to have an effective influence upon satisfaction with scalp care because of efficiently preventing hair damage against chemical treatment given the thermal permanent procedure in the damaged hair and of having anti-inflammatory and antibacterial action. Hence, Solanum Nigrum Fruit Extract is prospected to likely have feasibility as a natural substance.
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