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Abstract ▼ AI-Helper

A green tea used in this experiment was cultivated at Bosung (Chonnam) and purchased from a domestic market. The extract at 5$0^{\circ}C$ water from the powder of green tea partitioned with chloroform and ethyl acetate. The resulting solution was further purified with a chromatographic co...

A green tea used in this experiment was cultivated at Bosung (Chonnam) and purchased from a domestic market. The extract at 5$0^{\circ}C$ water from the powder of green tea partitioned with chloroform and ethyl acetate. The resulting solution was further purified with a chromatographic column (4.6$\times$250 mm, 15${\mu}{\textrm}{m}$, Lichrospher 100RP-18). Finally separation was achieved on a $\mu$-Bondapak $C_18$(3.9$\times$300mm, 10${\mu}{\textrm}{m}$) column. The elution order of the catechin compounds contained in the green tea was EGC(Epigallocatechin, C(catechin), EC(Epicatechin), EGCG(Epigallocatechin Gallate) and ECG(Epicatechin Gallate). From the experimental results the mobile phase for isolating EGCG from the extract consisted of 0.1% acetic acid in water/acetonitrile, 87/13%(v/v). The flow rate of mobile phase was 1.0 $m\ell$/min, and UV wavelength was fixed at 280 nm. 121.3 mg of EGCG, higher than 98% of purity, was obtained from 5 g of dry green tea.

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