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논문 상세정보

가시오가피 추출물의 항산화활성 및 MG-63 조골세포 증식과 alkaline phosphatase 활성에 미치는 효과

Antioxidant and Cell Proliferation Effects of Acanthopanax senticosus for Extract in Human Osteoblast-like MG-63 Cell Line

초록

본 연구에서는 가시오가피 추출물의 부위, 추출 용매, 산 가수분해 전처리에 따른 항산화 활성을 알아보고자 FRAP assay를 실시하였으며, 총 페놀 함량을 측정하고, 가시오가피 추출물의 항산화 활성과 총 페놀 함량간의 상관관계를 분석하였다. 또한 가시오가피 추출물 처리가 조골세포 증식률과 alkaline phosphatase 활성에 미치는 영향을 분석하여 가시오가피의 골다공증 예방 가능성을 규명하고자 하였다. 연구결과 가시오가피에 함유된 총 페놀 함량과 FRAP법으로 측정된 항산화 활성 간에 양의 상관관계가 있는 것으로 나타났으므로, 가시오가피의 항산화 활성은 함유된 폴리페놀에 의해 나타나는 것으로 풀이된다. 그러나 가시오가피에 함유된 폴리페놀 함량과 항산화 활성은 가시오가피의 부위와 추출 용매 및 산 처리 여부에 따라 차이가 나는 것으로 나타났다. 또한 가시오가피의 에탄올 추출물이 물 추출물에 비하여 조골세포 증식을 유의적으로 증가시켜주는 것으로 나타났다. 가시오가피의 물 추출물은 조골세포에서 염기성 인산 분해 효소의 활성을 유의적으로 증가 시켰다. 이는 가시오가피의 부위와 추출 용매 및 전처리 과정에 따라 추출되는 활성 성분의 종류와 양에 차이가 있기 때문으로 풀이된다. 이 결과는 가시오가피의 가공법을 선정하는데 고려되어야 할 중요한 사항으로 사료된다. 본 연구를 통하여 가시오가피의 항산화 활성과 조골세포 증식 효과가 제시되었다. 한국산 가시오가피를 건강 기능 식품 소재로 개발하기 위해서는 동물 실험과 인체 시험을 통한 심도 있는 연구가 수행되어야 할 것이다.

Abstract

Acanthopanax senticosus is a common Asian herb also known as "Siberian Ginseng". It is often used as a traditional herbal medicine for reducing damage in the liver, kidney, bone and muscle. In the present study we investigated the ferric reducing/antioxidant power and total phenolic contents of the ethanol-/water-extracts obtained from the stems and leaves of Acanthopanax senticosus. Osteoblast cellular proliferation was evaluated using the MTT and alkaline phosphatase activity assays in the human osteoblast-like MG-63 cell line. Acanthopanax senticosus extracts exerted remarkable ferric reducing/antioxidant power and contained high amount of phenolics. Among the extracts the stem-/ethanol-extract showed the highest antioxidant activity and total phenol content. Interestingly a highly positive correlation was found between antioxidant activity and total phenol content (p < 0.01). Proliferation of MG-63 osteoblast cells was highest in the stem-/ethanol-extract and alkaline phosphatase activity significantly increased in the water-extract of the stems (p < 0.05). These findings suggest that Acanthopanax senticosus extracts have antioxidant activity for preventing oxidative stress-related diseases and may have beneficial effects on bone health through the proliferation of osteoblast cells.

참고문헌 (34)

  1. Lee YS, Jung SH, Lim SS, JJ, Lee SH, Shin KH. Effects of the water extract from the stem bark of Acanthopanax senticosus on hyperlipidemia in rats. Korean J. Pharmacogn. 32: 103-107 (2001) 
  2. Kim SK, Kim YG, Lee MK, Han JS, Lee JH, Lee HY. Comparision of biological activity according to extracting solvents of four acanthopanax root bark. Korean J. Med. Crop Sci. 8: 21-28 (2000) 
  3. Kao KB. Chinese Ciwujia Studies. Heilongjiang Institute of Traditional Chinese Medicine, Harbin, China. p. 2 (1981) 
  4. Kim YM. Pharmaceutical comparison of ginseng with Acanthopanax from the aspect of oriental medicine. Korean J. Pharmacogn. 8: 131-138 (1977) 
  5. Brekhmann II, Dardymov IV. New substances of plant origin which increase nonspecific resistance. Annu. Rev. Pharmacol. 9: 419-430 (1969) 
  6. Lee KH, Nam JO, Yoon WH. Effect of protein-bound polysacharide isolated from Acanthopanax senticosus in reducing the toxic effect of cisplatin. Korean J. Pharmacog. 38: 1-17 (2007) 
  7. Hacker B, Medon PJ. Cytotoxic effects of Eleutherococcus senticosus aqueous extracts in combination with N6-(delta 2-isopentenyl)- adenosine and 1-beta-D-arabinofuranosylcytosine against L1210 leukemia cells. J. Pharm. Sci. 73: 270-282 (1984) 
  8. Bohn B, Nebe CT, Birr C. Flow-cytometric studies with Eleutherococcus senticosus extract as an immunomodulatory agent. Drug Res. 37: 1193-1196 (1987) 
  9. Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S. Protective effects of Acanthopanax senticosus harms from Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol. Pharm. Bull. 19: 1227-1230 (1996) 
  10. Kim CJ, Hahn DR. The biological activity of a new glycoside, chiisanoside from Acanthopanax chiisanensis nakai leaves. Yakhak Hoeji 24: 123-134 (1980) 
  11. Yook CS, Rho YS, Sed SH, Leem JY, Han SH. Chemical compounds of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40: 215-261 (1996) 
  12. Frei B. Natural Antioxidants in Human Health and Disease. Academic Press, San Diago, CA, USA. pp. 1-21 (1994) 
  13. Seo JH, Lim BO, Kang SA, Han IK, Park DK. The effect of P diet on osteoporosis induced by ovariectomy in rats. J. Korean Soc. Menopause 8: 76-86 (2002) 
  14. Kim KS, Min BK, Lee SP, Kim IS, Kim HY, Sim JR. Evaluation of biological markers of bone turnover in postmenopausal osteophorotic women to alendronate treatment. J Korean Soc. of Menopause 6: 36-42 (2000) 
  15. Rogers J. Estrogens in the menopause and postmenopause. New Engl. J. Med. 280: 364-367 (1967) 
  16. Duda RJ, O'Brien JF, Katzmann JA. Concurent assays of circulating bone-gla protein and alkaline phosphate: Effects of sex, age, and metabolic bone disease. J. Clin. Endocr. Meta. 66: 951-957 (1988) 
  17. Bowles SA, Kurdy N, Davis AM, Franse MW, Marsh DR. Serum osteocalcin, total and bone-specific alkaline phosphate following isolated tibial shaft fracture. Ann. Clin. Biochem. 33: 196-200 (1996) 
  18. Anderson JJ, Garner SC. Phytoestrogen and bone. Bailliere Clin. Endoc. 12: 543-557 (1998) 
  19. Setchell KDR, Cassidy A. Dietary isoflavones: Biological effects and relevance to human health. J. Nutr. 129: 758S-767S (1999) 
  20. Lee HK. Effect of black bean and samryungbakchulsan on ovariectomy- induced postmenopausal osteoporotic rats. PhD thesis. Kyung Hee University, Seoul, Korea (1998) 
  21. Ryu IC, Lee Ym, Koo Y, Bae KW, Chung CP. Effect of safflower extracts on activity of PDL cells and MG-63 cells. J. Korean Acad. Periodontol. 27: 867-882 (1997) 
  22. Ho JY. Cell proliferation effects of eucommiae cortex and Acanthopanacs cortex methanol extraxts on the osteoblast-like cell line, MG-63 and Saos-2. PhD thesis. Kyung Hee University, Seoul, Korea (2001) 
  23. Kim MR, Yang CH, Seo BI. Effects of safflower seeds on bone mineral density in ovarientomy-induced postmenopausal osteoporotic rats. Korean J. Herbol. 13: 37-43 (1998) 
  24. Yang DS, Cha MH, Kang BJ, O SU, Kim YE, Yun YS. A study on the longitudinal bone growth of growth-stimulating material with Eleutherococcus senticosus. Korean J. Food Sci. Technol. 35: 702-707 (2003) 
  25. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP assay. Anal. Biochem. 239: 70-86 (1996) 
  26. Cho YH, Park SJ, Shin HJ, Jang KH, Kang SA, Choue RW. Comparative estrogenic effects of 'Yack-kong' and soy bean on the proliferation of human osteoblastic cell line, MG-63. Korean J. Nutr. 34: 905-911 (2001) 
  27. Puzas JE, Brand JS. Bone cell phosphotyrosine phosphatase: Characterization and regulation by calcitropic hormones. Endocrinology 116: 2463-2468 (1985) 
  28. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59: 527-605 (1979) 
  29. Yu MH, Im HG, Lee HJ, Ji YJ, Lee I-S. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis rehder. Korean J. Food Sci. Technol. 38: 128-134 (2006) 
  30. Kim LH, Han SS, Choi YS. Antioxidant effect of the extracts of Acanthopanax senticosus. Korean J. Pharmacogn. 33: 359-363 (2002) 
  31. Lee JH, Lee SR. Analysis of phenolic substances content in Korean plant food. Korean J. Food Sci. Technol. 26: 310-316 (1994) 
  32. Choi SY, Lim SH, Kim JS, Ha TY, Kim SR, Kang KS, Hwang IK. Evaluation of estrogenic and antioxidant activity of some edible and medicinal plants. Korean J. Food Sci. Technol. 37: 549-556 (2005) 
  33. Kim MJ, Kwon YS, Yu CY. Antioxidative compounds in extracts of Eleutherococcus senticosus max. plantlets. Korean J. Med. Corp. Sci. 13: 194-198 (2005) 
  34. Davydov M, Krikorian AD. Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen: A closer look. J. Ethnopharmacol. 72: 345-393 (2000) 

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  2. Choi, Seung-Min ; Park, Jong-Bong ; Kim, Jong-Min ; In, Kyung-Min ; Park, Heon-Yong 2008. "Acanthopanax senticosus Extract Acts as an Important Regulator for Vascular Functions." 생명과학회지 = Journal of life science, 18(5): 701~707 
  3. Lee, Yim-Jin ; Han, Jin-Suk 2009. "Physicochemical and Sensory Characteristics of Traditional Doenjang Prepared using a Meju Containing Components of Acanthopanax senticosus, Angelica gigas, and Corni fructus" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 25(1): 90~97 
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  11. Yu, Seok-Yeong ; Lee, Young-Jun ; Song, Ho-Seong ; Hong, Hee-Do ; Lim, Jeong-Ho ; Choi, Hyeon-Son ; Lee, Boo-Yong ; Kang, Suk-Nam ; Lee, Ok-Hwan 2012. "Antioxidant Effects and Nitrite Scavenging Ability of Extract from Acanthopanax cortex Shoot" 한국식품영양학회지 = The Korean journal of food and nutrition, 25(4): 793~799 
  12. Kim, Young-Hyun ; Bae, Da-Bin ; Park, Sun-Ok ; Lee, Sang-Jong ; Cho, Ok-Hyun ; Lee, Ok-Hwan 2013. "Method Validation for the Determination of Eleutherosides and β-Glucan in Acanthopanax koreanum" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(9): 1419~1425 
  13. Kim, Dong-Min ; Jin, Bora ; Kim, Ji Yeon 2014. "Comparative Radical Scavenging and Nitric Oxide Production Capacity of Water Extracts of Acanthopanax divaricatus var, Albeofructus and Eleutherococcus divaricatus var. Chiisanensis" Journal of applied biological chemistry, 57(4): 337~340 
  14. Lee, Kyung-Seon ; Choi, Eun-Sik ; Han, Sang-Yong ; Kim, Yun-Kyung 2014. "Inhibitory Effect of Water Extracts of Aconiti Lateralis Preparata Radix and Acanthopanacis Cortex on Differentiation of Bone Marrow-Derived Adipocytes and Osteoclasts" 大韓韓醫學方劑學會誌 = Herbal formula science, 22(1): 151~165 
  15. 2014. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 39(2): 121~128 

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