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논문 상세정보

초록

인삼사포닌 ginsenoside는 인삼의 주요한 약리성분으로 인삼을 경구투여 시 major 사포닌의 생체 내에서의 흡수는 매우 낮아 인삼사포닌의 약효를 증대시키기 위해서 기존에 많이 존재하는 major 사포닌을 상대적으로 흡수도 잘 되며 약효도 더 뛰어난 minor 사포닌으로의 전환이 요구된다. 본 연구는 김치에서 분리한 ${\beta}-glucosidase$ 활성균주 L. brevis LH8이 분비하는 효소를 이용하여 ginsenoside Rd를 compound K로 전환시켰다. L. brevis LH8의 효소액은 반응 온도 $30^{\circ}C$에서 효소활성이 가장 좋았고 $35^{\circ}C$이상에서는 활성이 급격히 저하되었으며, pH $6.0{\sim}12.0$ 사이에서 효소활성이 가장 좋았고, pH 5.0 이하 및 pH 13.0 이상에서는 활성도가 떨어지는 것을 관찰할 수 있었다. 또한 ginsenoside Rd는 반응 48시간부터 ginsenoside F2로 전환되기 시작하였으며, 반응 72 시간 이후에는 대부분 compound K로 전환되었다.

Abstract

Ginsenosides have been regarded as the principal components responsible for the pharmacological and biological activities of ginseng. Absorption of major ginsenosides from the gastrointestinal tract is extremely low, when ginseng is orally administered. In order to improve absorption and its bioavailability, conversion of major ginsenosides into more active minor ginsenoside is very much required. Here, we isolated lactic acid bacterium (Lactobacillus brevis LH8) having ${\beta}-glucosidase$ activity from Kimchi. Bioconversion ginsenoside Rd by this bacterium in different temperatures was investigated. The maximum activities of crude enzymes precipitated by ethanol were shown in $30^{\circ}C$ and then gradually decreased. In order to compare the effect of pH, the crude enzymes of L. brevis LH8 were mixed in 20mM sodium phosphate buffer (pH 3.5 to pH 8.0) and reacted ginsenoside Rd. Ginsenoside Rd was almost hydrolyzed between pH 6.0 and pH 12.0, but not hydrolyzed under pH 5.0 and above pH 13.0. Ginsenoside Rd was hydrolyzed after 48 h incubation, whereas ginsenoside F2 appeared from 48 h to 72 h, and ginsenoside Rd was almost converted into compound K after 72 h.

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이 논문을 인용한 문헌 (10)

  1. Joung, Eun-Mi ; Hwang, In-Guk ; Lee, Hyeon-Yong ; Jeong, Jae-Hyun ; Yu, Kwang-Won ; Jeong, Heon-Sang 2009. "Changes of Saponin and β-Glucan Content on the Cultured Ginseng with Mushroom Mycelia" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 38(8): 1084~1089 
  2. 2010. "" Journal of ginseng research = 高麗人參學會誌, 34(2): 104~112 
  3. Lee, Ka-Soon ; Seong, Bong-Jae ; Kim, Gwan-Hou ; Kim, Sun-Ick ; Han, Seung-Ho ; Kim, Hyun-Ho ; Baik, Nam-Doo 2010. "Ginsenoside, Phenolic Acid Composition and Physiological Significances of Fermented Ginseng Leaf" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(8): 1194~1200 
  4. 2011. "" 韓國資源植物學會誌 = Korean journal of plant resources, 24(6): 712~718 
  5. Park, Kwang-Hyun ; Kim, Young-Seon ; Jeong, Jae-Hun 2011. "Inhibitory Effects of Ginseng Extracts on Histamine-release from Rat's Mast Cell" 韓國資源植物學會誌 = Korean journal of plant resources, 24(1): 98~104 
  6. Im, Ga-Young ; Ma, Jin-Yeul ; Kim, Kun-Woo ; Choi, Jin-Kook ; Kang, Dong-Kyoon ; Kwon, Tae-Ryoung ; Jang, Se-Young ; Jeong, Yong-Jin 2011. "Quality Characteristics of 4 Year-old Ginseng by Enzymatic Hydrolysis Conditions" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(2): 229~234 
  7. Han, Gang ; Lee, Nam-Keun ; Lee, Yu-Ri ; Jeong, Eun-Jeong ; Jeong, Yong-Seob 2012. "Optimization of Compound K Production from Ginseng Extract by Enzymatic Bioconversion of Trichoderma reesei" 한국식품영양학회지 = The Korean journal of food and nutrition, 25(3): 570~578 
  8. Kang, Bok-Hee ; Lee, Kun-Jae ; Hur, Sang-Sun ; Lee, Dong-Sun ; Lee, Sang-Han ; Shin, Ki-Sun ; Lee, Jin-Man 2013. "Ginsenoside derivatives and quality characteristics of fermented ginseng using lactic acid bacteria" 한국식품저장유통학회지 = Korean journal of food preservation, 20(4): 573~582 
  9. Lee, Dong-Geun ; Park, Seong-Hwan ; Lee, Sang-Hyeon 2013. "Comparison of Growth Inhibitory Effects on Cancer Cells of Saponin and Fucoidan Treated with Recombinant Thermophilic Xylose Isomerase" KSBB Journal, 28(5): 332~337 
  10. 2014. "" Mycobiology, 42(4): 368~375 

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