[논문]모르핀 자가투여에서 신문혈 자침효과의 지속 양상

이봉효; 박서영; 이지혜; 임성철; 김재수; 이윤규; 정태영; 양재하; 윤성순; 이경민

[국내논문] 모르핀 자가투여에서 신문혈 자침효과의 지속 양상
Duration Pattern of the Effect of Acupuncture at $HT_7$ in Morphine Self-administration 원문보기

大韓鍼灸學會誌= The journal of Korean Acupuncture & Moxibustion Society, v.27 no.4, 2010년, pp.55 - 65

초록
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목적 : 기존 연구에서 신문혈 자침은 알코올 및 코카인을 자가섭취하는 동물 모델에서 효과적이라는 점이 밝혀졌으며, 또한 모르핀 자가섭취를 억제할 수 있음이 밝혀졌다. 이러한 결과를 바탕으로 본 연구에서는 자침의 효과가 얼마나 지속될 수 있는지 알아보았다. 재료 및 방법 : 체중 270~300g의 수컷 SD계 흰쥐를 이용하였다. 먹이섭취 훈련을 통과한 후 오른쪽 경정맥에 관을 삽입하는 수술을 거쳐, 0.1mg/kg의 모르핀을 매일 1시간, 총 3주 동안 자가섭취 하도록 하였다. 모르핀을 일정하게 섭취한 동물에게는 다음날 침술을 시행하였다. 두 번째 실험에서는 GABAA 및 GABAB 길항제를 자침 30분 전에 투여하여 침술의 효능과 GABA 수용체계 사이의 관계를 검증하였다. 결과 : 모르핀 자가섭취를 억제하는 신문혈 자침의 효과는 매일 비슷하게 나타나지 않았으며, 4일째에는 유의한 효과가 없는 것으로 나타났다. 그러나 5일째와 6일째에는 다시 유의한 효과가 나타나 뒤집어진 U 자형 곡선을 나타내었다. 또한 GABA 수용체의 길항제들은 자침의 효과가 유의하게 나타났을 때 이를 차단하는 결과를 보였다. 결론 : 모르핀 중독 치료에서 침술의 효과는 항상 비슷하게 발휘되는 것이 아니므로 침 치료는 적어도 1주일에 2회 이상 받을 필요가 있다. 아울러 보다 심도 있는 연구를 위하여 뇌신경 체계에 대한 연구가 이루어질 필요가 있는 것으로 사료된다.

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제안 방법

However, most of them just have revealed the effectiveness of acupuncture, and there is little study that has investigated the duration of effectiveness. Based on this, it was investigated how long the effect of acupuncture to suppress morphine taking would last.
the house lights on a wall, and cue lights above the active lever on the opposite wall. The signal of active lever-press was delivered to computer installed schedule manager program and the motor equipped with the syringe of morphine solution was operated according to the experiment design.
In this study, both antagonists reversed the effect of acupuncture in the front or the rear showing the best on the 1^st day, and there was no reverse on the 4^th day(Fig. 8). This pattern of reverse is thought to be due to the unique duration pattern of the effect of acupuncture, i.

대상 데이터

Male Sprague-Dawley rats(Daehan Animal, Seoul, Korea) weighing 270～300g at the beginning of the experiment, were used. Rats were housed in home cage of ad libitum food and water and a 12h light-dark cycle except the 1^st day of the food training on which animals were kept under overnight schedule.

데이터처리

Schedule manager program(Med Associates, St. Albans, VT) collected data of lever responses, and SPSS statistics program analyzed data using oneway analysis of variance(ANOVA) and post hoc test with Tukey for the 1^st experiment and the least significant difference(LSD) for the 2^nd experiment. The statistical significance was regarded with the p value less than 0.
of infusions. One-way ANOVA and post hoc Tukey test. Rats of HT₇ group(n=8) were given acupuncture at bilateral HT₇ points of heart channel, and LI₅ group(n=10) received at LI₅ points of large intestine channel.

성능/효과

Animals were trained to selfadminister morphine solution under daily 1h schedule except the 1^st day on which they were exposed during 4h to facilitate lever press for morphine taking. After 3weeks of training, the responses of active lever were checked, and animals who had taken stably(establishment of baseline : variation of active lever responses for 3 consecutive sessions less than 20% of the mean) received test.
The results of present study show that the basal level of active lever response was 16.13±1.27 for control group, 12.63±0.96 for HT7 group, 12.84±0.60 for LI5 group.
The results of the present study show that the active lever response was 15.30±1.47 for control group, 7.50±1.64 for HT7 group, 11.80±1.47 for LI5 group on the 1st test day, and 15.40±1.35 for control group, 8.38±1.51 for HT7 group, 11.90±1.35 for LI5 group on the 2nd test day, and 16.60±1.51 for control group, 9.00±1.69 for HT7 group, 12.50±1.51 for LI5 group on the 3rd test day, and 17.60±2.15 for control group, 11.00±2.41 for HT7 group, 12.90±2.15 for LI5 group on the 4th test day, and 16.80±2.25 for control group, 8.13±2.51 for HT7 group, 12.70±2.25 for LI5 group on the 5th test day, and 16.90±1.96 for control group, 8.38±2.19 for HT7 group, 12.20±1.96 for LI5 group on the 6th test day, and 15.50±1.30 for control group, 9.25±1.45 for HT7 group, 11.40±1.30 for LI5 group on the 7th test day.
3) Effect of acupuncture on the inactive lever response The inactive lever response was 2.56±1.18 for control group, 3.88±1.25 for HT7 group, 5.40±1.12 for LI5 group on the 1st test day, and 3.22±1.22 for control group, 3.25±1.29 for HT7 group, 3.40±1.15 for LI5 group on the 2nd test day, and 3.11±0.85 for control group, 2.50±0.91 for HT7 group, 2.90±0.81 for LI5 group on the 3rd test day, and 4.67±1.35 for control group, 3.25±1.44 for HT7 group, 4.40±1.28 for LI5 group on the 4th test day, and 1.33±1.04 for control group, 3.88±1.11 for HT7 group, 3.50±0.99 for LI5 group on the 5ㅊ test day, and 4.22±1.49 for control group, 5.63±1.58 for HT7 group, 2.00±1.42 for LI5 group on the 6th test day, and 2.00±1.44 for control group, 2.50±1.53 for HT7 group, 3.80±1.37 for LI5 group on the 7th test day.
The results of the present study show that the percent of active lever compared to basal level was 97.51±7.06% for control group, 50.73±9.07% for HT7 group, 92.74±6.55% for LI5 group on the 1st day, and 95.01±10.49% for control group, 62.66±8.02% for HT7 group, 93.23±5.69% for LI5 group on the 2 day, and 102.71±5.96% for control group, 73.40± 8.84% for HT7 group, 93.71±11.13% for LI5 group on the 3rd day, and 104.51±10.56% for control group, 84.95±14.42% for HT7 group, 99.66±9.69% for LI5 group on the 4th day, and 100.35±8.62% for control group, 65.78±12.10% for HT7 group, 105.12±11.86% for LI5 group on the 5th day, and 97.18±7.69% for control group, 65.23±11.69% for HT7 group, 92.64± 7.97% for LI5 group on the 6th day, and 96.33±4.48% for control group, 70.54±15.86% for HT7 group, 89.22± 6.58% for LI5 group on the 7th day.
The significant differences were HT7 + bicuculline group vs. SCH 50911 group(+ : p<0.05) on the 1st day, HT7 + bicuculline group vs. bicuculline group(‡: p<0.05), bicuculline alone group vs. SCH 50911 alone group(& : p<0.05) on the 2nd day, HT7 group vs. bicuculline group(@ : p<0.05), HT7 group vs. SCH 50911 group($ : p<0.05) on the 3rd day, HT7 group vs. bicuculline group(@@ : p<0.01), HT7 + bicuculline group vs. bicuculline group(‡‡‡: p<0.001), bicuculline group vs. SCH 50911 group(& : p<0.05), HT7 + SCH 50911 group vs. bicuculline group(%% : p< 0.01) on the 4th day, HT7 group vs. HT7 + bicuculline group(* : p<0.05), HT7 + bicuculline group vs. bicuculline group(‡ : p<0.01), HT7 + SCH 50911 group vs. bicuculline group(% : p<0.05) on the 5th day, HT7 group vs. bicuculline group(@@ : p<0.01), HT7 + bicuculline group vs. bicuculline group(‡: p<0.05), bicuculline group vs. SCH 50911 group(& : p<0.05), HT7 + SCH 50911 group vs. bicuculline group(%% : p<0.01) on the 7th day(Fig. 7).
As a conclusion, the results of this study suggest that the effect of acupuncture at HT₇ to suppress morphine taking is not constant, and may be weakened or disappear after some treatments. Therefore it is needed to take acupuncture treatment at least twice per a week.

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