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논문 상세정보

참나물의 항균, 항산화 활성 및 대장암세포 성장억제 활성 평가

Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa

초록

참나물은 광범위하게 이용되는 식용산채임에도 불구하고 그 연구는 전 세계적으로 미미한 상태이다. 본 연구에서는 최근 고급 식용 산채로서 각광받고 있는 참나물의 메탄올 추출물을 조제하고, 이로부터 n-hexane, methylene chloride, ethylacetate 및 butanol을 이용하여 순차적 유기 용매 분획물과 물 잔류물을 조제하여 각각의 항산화, 항균 및 대장암세포 생육억제 활성을 평가하였다. 참나물 메탄올 추출물의 71.51%는 물 잔류물로 이행되어 추출물 대부분이 수용성 물질이었으며, n-hexane, methylene chloride, ethylacetate 및 butanol 분획 효율은 각각 18.71, 0.7, 0.56, 및 4.57%로 나타났다. 총 플라보노이드 및 총 폴리페놀 함량은, 분획수율이 가장 낮은 ethylacetate 분획에서 가장 높았으며 물 잔류물에서 가장 낮았다. 항균 활성 평가결과, ethylacetate 분획에서 그람양성 및 그람음성세균에 대해 광범위한 항균 활성을 나타내었으며, 특히 그람양성세균에는 n-hexane 분획이, 그람음성세균에는 ethylacetate 분획이 효과적이었다. 항산화 활성의 경우 ethylacetate 및 butanol 분획에서 양호한 DPPH 및 ABTS 소거능, 환원력을 나타내었으며, n-hexane및 methylene chloride 분획에서 우수한 nitrite 소거능을 나타내었다. 한편 butanol 분획에서는 다른 분획과는 달리 우수한 인간 대장암세포 생육억제 활성을 나타내었다. 본 연구 결과는 참나물을 이용한 기능성 식품개발 및 ethylacetate 분획을 대상으로 한 유용 소재개발의 기본 자료로 활용될 것이다.

Abstract

Pimpinella brachycarpa, called as cham-na-mul in Korea, is an edible popular herb. However, the study of biological activity of P. brachycarpa is still rudimentary in worldwide. In this study, from the cultivated P. brachycarpa, we prepared the methanol extract and its subsequent solvent fractions, and their antimicrobial, antioxidation, and anti-proliferative activities were evaluated. The fraction yields of n-hexane (H), methylene chloride (EC), ethylacetate (EA), butanol (B), and water residue (W) from the methanol extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of total flavonoid and total polyphenol showed that the EA fraction contained the highest contents (89.23 and 200 mg/g), and the W residue has the lowest contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity assay, the EA fraction showed a broad-range antibacterial activity, while the H fraction is effective against gram positive bacteria. In antioxidation activity assay, EA and B fraction showed strong DPPH anion and ABTS cation scavenging activities including reducing power, and Hand MC fraction showed effective nitrite scavenging activity (71.43~83.82 ${\mu}g$/mL of $IC_{50}$). In a while, only B fraction showed strong anti-proliferative activity against human colorectal cancer HCT-116 (166 ${\mu}g$/mL of $IC_{50}$) as a dose-dependent manner up to 200 ${\mu}g$/mL. These results suggest that the EA and B fraction of P. brachycarpa could be developed as functional food ingredients.

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이 논문을 인용한 문헌 (3)

  1. 2013. "" Bulletin of the Korean Chemical Society, 34(7): 2215~2217 
  2. Jeong, Ji-Suk ; Kim, Yong-Joo ; Choi, Bo-Rum ; Park, No-Jin ; Son, Byeong-Gil ; Kwak, Young-Se ; Kim, Jong-Cheol ; Cho, Kyoung-Hwan ; Kim, In-Ho ; Kim, Seong-Ho 2013. "Physicochemical Changes in Hemerocallis coreana Nakai After Blanching, Drying, and Fermentation" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(10): 1638~1648 
  3. Jeong, Ji-Suk ; Kim, Yong-Joo ; Choi, Bo-Rum ; Go, Geun-Bae ; Son, Byeong-Gil ; Gang, Suk-Won ; Moon, Seung-Man 2014. "Antioxidant and Physicochemical Changes in Salvia plebeia R. Br. after Hot-air Drying and Blanching" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 43(6): 893~900 

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