[국내논문]대식세포에서 LPS로 유도된 염증에 대한 회향 열매의 항염 효과 Anti-inflammatory Effects of the Fruits of Foeniculum vulgare in Lipopolysaccharide-stimulated Macrophages원문보기
회향(Foeniculum vulgare) 열매의 메탄올 추출물과 분획물(hexane, methylene chloride, ethylacetate, n-butanol)을 cell culture model system을 이용하여 항염증 활성을 평가하였다. 이를 위해, LPS처리로 염증이 유도된 RAW 264.7 macrophage에서 시료가 세포독성을 나타나지 않는 $100{\mu}g/ml$의 농도를 처리한 후 염증매개물질인 NO, PGE2, 염증성 사이토카인($IL-1{\beta}$, IL-6 및 TNF-${\alpha}$) 생성 및 mRNA 발현을 측정하였다. 그 결과 hexane, methylene chloride 및 ethylacetate 분획물은 대조군에 비해 NO, PGE2 생성을 억제하였으며, 이는 iNOS, COX-2 mRNA 발현 저해에서 기인함을 확인하였다. Methylene chloride와 ethylacetate 분획물은 $100{\mu}g/ml$의 농도에서 $IL-1{\beta}$, IL-6 생성 및 mRNA 발현을 효과적으로 억제하였으며, 특히 ethyl acetate 분획물은 TNF-${\alpha}$ 의 생성과 mRNA발현을 유의적으로 저해하였다. 본 연구 결과를 통해 회향 및 회향 분획물의 항염증 활성을 확인하였으며, 이는 염증 매개물질인 NO, PGE2 그리고 염증성 사이토카인 mRNA 활성 및 분비 억제에 의한 것으로 나타났다.
회향(Foeniculum vulgare) 열매의 메탄올 추출물과 분획물(hexane, methylene chloride, ethylacetate, n-butanol)을 cell culture model system을 이용하여 항염증 활성을 평가하였다. 이를 위해, LPS처리로 염증이 유도된 RAW 264.7 macrophage에서 시료가 세포독성을 나타나지 않는 $100{\mu}g/ml$의 농도를 처리한 후 염증매개물질인 NO, PGE2, 염증성 사이토카인($IL-1{\beta}$, IL-6 및 TNF-${\alpha}$) 생성 및 mRNA 발현을 측정하였다. 그 결과 hexane, methylene chloride 및 ethylacetate 분획물은 대조군에 비해 NO, PGE2 생성을 억제하였으며, 이는 iNOS, COX-2 mRNA 발현 저해에서 기인함을 확인하였다. Methylene chloride와 ethylacetate 분획물은 $100{\mu}g/ml$의 농도에서 $IL-1{\beta}$, IL-6 생성 및 mRNA 발현을 효과적으로 억제하였으며, 특히 ethyl acetate 분획물은 TNF-${\alpha}$ 의 생성과 mRNA발현을 유의적으로 저해하였다. 본 연구 결과를 통해 회향 및 회향 분획물의 항염증 활성을 확인하였으며, 이는 염증 매개물질인 NO, PGE2 그리고 염증성 사이토카인 mRNA 활성 및 분비 억제에 의한 것으로 나타났다.
Foeniculum vulgare has long been prescribed in traditional medicine for the treatment of inflammation diseases. In this study, we aimed to investigate the inhibitory effects of the fruits of F. vulgare on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells under non-cytotoxic ($100{\...
Foeniculum vulgare has long been prescribed in traditional medicine for the treatment of inflammation diseases. In this study, we aimed to investigate the inhibitory effects of the fruits of F. vulgare on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells under non-cytotoxic ($100{\mu}g/ml$) conditions. The 80% methanol extract was subsequently partitioned successively with hexane, methylene chloride, ethyl acetate, and n-butanol, and the fractions so obtained were also examined for their anti-inflammatory effects. Among them, the hexane, methylene chloride, and ethyl acetate fractions inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS stimulated macrophages. The methylene chloride and ethyl acetate fractions also suppressed the productions of interleukin $(IL)-1{\beta}$ and IL-6 by down-regulating their mRNA levels in LPS stimulated RAW 264.7 cells. Furthermore, the ethyl acetate fraction strongly suppressed tumor necrosis factor (TNF)-${\alpha}$ at the protein and mRNA levels in LPS stimulated RAW 264.7 cells. These observations suggest that the anti-inflammatory actions of F. vulgare are due to inhibitions of the productions of NO, PGE2, and pro-inflammatory cytokines.
Foeniculum vulgare has long been prescribed in traditional medicine for the treatment of inflammation diseases. In this study, we aimed to investigate the inhibitory effects of the fruits of F. vulgare on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells under non-cytotoxic ($100{\mu}g/ml$) conditions. The 80% methanol extract was subsequently partitioned successively with hexane, methylene chloride, ethyl acetate, and n-butanol, and the fractions so obtained were also examined for their anti-inflammatory effects. Among them, the hexane, methylene chloride, and ethyl acetate fractions inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS stimulated macrophages. The methylene chloride and ethyl acetate fractions also suppressed the productions of interleukin $(IL)-1{\beta}$ and IL-6 by down-regulating their mRNA levels in LPS stimulated RAW 264.7 cells. Furthermore, the ethyl acetate fraction strongly suppressed tumor necrosis factor (TNF)-${\alpha}$ at the protein and mRNA levels in LPS stimulated RAW 264.7 cells. These observations suggest that the anti-inflammatory actions of F. vulgare are due to inhibitions of the productions of NO, PGE2, and pro-inflammatory cytokines.
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제안 방법
In view of the small amount known about the anti-inflammatory activity of F. vulgare, we evaluated the effects of its methanolic extract and of other fractions on NO levels and PGE2 cytokine levels at the protein and mRNA levels in lipopolysaccharide-stimulated RAW 264.7 macrophages.
대상 데이터
Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and streptomycin-penicillin were purchased from GIBCO (Grand Island, NY, USA).
RAW 264.7 mouse macrophages were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and used for all studies. Cells were cultured in DMEM containing 10% heat-inactivated FBS, 100 units/ml penicillin, and 100 ng/ml streptomycin at 37℃ in a 5% CO2 humidified atmosphere.
데이터처리
Data were analyzed by one-way ANOVA followed by Duncan's multiple range test using GraphPad Prism 4.0 software (GraphPad Software, San Diego, CA, USA).
이론/모형
Cytotoxicity was assessed using a colorimetric assay (XTT Cell Proliferation Kit), which was based on the metabolization of XTT to formazan by mitochondrial dehydrogenase in living cells. Briefly, attached cells were treated with each extract for 18 hr, 50 μl of XTT solution was then added, and cells were incubated for an additional 4 hr.
7 cells were treated with each sample (100 μg/ml) for 18 hr. Cell viabilities were evaluated using an XTT colorimetric assay. Results are expressed as the means ± SDs of three independent experiments.
참고문헌 (34)
Huang, H., Park, P. H., McMullen, M. R. and Nagy, L. E. 2008. Mechanisms for the anti-inflammatory effects of adiponectin in macrophages. J Gastroenterol Hepatol 1, 50-53.
Choi, S. Y., Hur, S. J., An, C. S., Jeon, Y. H., Jeoung, Y. J., Bak, J. P. and Lim, B. O. 2010. Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate. J Biomed Biotechnol 2010, 943516.
Jeong, D. H., Kim, K. B., Kim, M. J., Kang, B. K. and Ahn, D. H. 2014. Anti-inflammatory activity of methanol extract and n-hexane fraction mojabanchromanol b from Myagropsis myagroides. Life Sci 114, 12-19.
Vaday, G. G. and Lider, O. 2000. Extracellular matrix moieties, cytokines, and enzymes: dynamic effects on immune cell behavior and inflammation. J Leukoc Biol 67, 149-159.
Luster, A. D., Alon, R. and von Andrian, U. H. 2005. Immune cell migration in inflammation: present and future therapeutic targets. Nat Immunol 6, 1182-1190.
Fordham, J. B., Naqvi, A. R. and Nares, S. 2014. Leukocyte production of inflammatory mediators is inhibited by the antioxidants phloretin, silymarin, hesperetin, and resveratrol. Mediators Inflamm 2014, 938712.
Yamamoto, Y. and Gaynor, R. B. 2001. Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer. J Clin Invest 107, 135-142.
Han, Y., Jung, H. W., Lee, D. H., Kwon, S. Y., Son, K. H. and Park, Y. K. 2013. Anti-inflammatory effects of prosapogenin III from the dried roots of Liriope platyphylla in LPS-stimulated RAW264.7 cells. J Asian Nat Prod Res 15, 1038-1049.
Calixto, J. B., Campos, M. M., Otuki, M. F. and Santos, A. R. 2004. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Med 70, 93-103.
Chun, S. C., Jee, S. Y., Lee, S. G., Park, S. J., Lee, J. R. and Kim, S. C. 2007. Anti-inflammatory activity of the methanol extract of moutan cortex in LPS-activated Raw264.7 cells. Evid Based Complement Alternat Med 4, 327-333.
Jeon, C. M., Shin, I. S., Shin, N. R., Hong, J. M., Kwon, O. K., Kim, H. S., Oh, S. R., Myung, P. K. and Ahn, K. S. 2014. Siegesbeckia glabrescens attenuates allergic airway inflammation in LPS-stimulated RAW 264.7 cells and OVA induced asthma murine model. Int Immunopharmacol 22, 414-419.
Song, M. and Park, H. J. 2014. Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages. J Ethnopharmacol 154, 311-318.
Araya, A. V., Pavez, V., Perez, C., Gonzalez, F., Columbo, A., Aguirre, A., Schiattino, I. and Aguillon, J. C. 2003. Ex vivo lipopolysaccharide (LPS)-induced TNF-alpha, IL-1beta, IL-6 and PGE2 secretion in whole blood from Type 1 diabetes mellitus patients with or without aggressive periodontitis. Eur Cytokine Netw 14, 128-133.
Shih, M. F., Chen, L. Y., Tsai, P. J. and Cherng, J. Y. 2012. In vitro and in vivo therapeutics of ${\beta}$ -thujaplicin on LPS-induced inflammation in macrophages and septic shock in mice. Int J Immunopathol Pharmacol 25, 39-48.
Zuckerman, S. H., Shellhaas, J. and Butler, L. D. 1989. Differential regulation of lipopolysaccharide-induced interleukin 1 and tumor necrosis factor synthesis: effects of endogenous and exogenous glucocorticoids and the role of the pituitary-adrenal axis. Eur J Immunol 19, 301-305.
Dawid-Pac, R. 2013. Medicinal plants used in treatment of inflammatory skin diseases. Postepy Dermatol Alergol 30, 170-177.
Wang, Q., Kuang, H., Su, Y., Sun, Y., Feng, J., Guo, R. and Chan, K. 2013. Naturally derived anti-inflammatory compounds from Chinese medicinal plants. J Ethnopharmacol 146, 9-39.
Liu, Z. G., Li, Z. L., Bai, J., Meng, D. L., Li, N., Pei, Y. H., Zhao, F. and Hua, H. M. 2014. Anti-inflammatory diterpenoids from the roots of Euphorbia ebracteolata. J Nat Prod 77, 792-799.
Soylu, S., Yigitbas, H., Soylu, E. M. and Kurt, S. 2007. Antifungal effects of essential oils from oregano and fennel on Sclerotinia sclerotiorum. J Appl Microbiol 103, 1021-1030.
Abdel-Fattah, M. E., Taha, K. E., Abdel Aziz, M. H., Missalem, A. A. and El-Khrisy, E. A. M. 2001. Chemical constituents of Citrus Limonia and Foeniculum vulgare. Indian J Heterocycl Chem 13, 45-48.
Choi, E. M. and Koo, S. J. 2004. Inhibition of lipopolysaccharide- stimulated inflammatory mediator production in RAW264.7 macrophages by Foeniculum vulgare fruit extract. Korean J Food Cook Sci 20, 505-510.
Coleman, J. W. 2001. Nitric oxide in immunity and inflammation. Int Immunopharmacol 1, 1397-1406.
Tripathi, P., Tripathi, P., Kashyap, L. and Singh, V. 2007. The role of nitric oxide in inflammatory reactions. FEMS Immunol Med Microbiol 51, 443-452.
Giuliano, F. and Warner, T. D. 2002. Origins of prostaglandin E2: involvements of cyclooxygenase (COX)-1 and COX-2 in human and rat systems. J Pharmacol Exp Ther 303, 1001-1006.
Pettus, B. J., Bielawski, J., Porcelli, A. M., Reames, D. L., Johnson, K. R., Morrow, J., Chalfant, C. E., Obeid, L. M. and Hannun, Y. A. 2003. The sphingosine kinase 1/sphingosine-1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha. FASEB J 17, 1411-1421.
Popa, C., Netea, M. G., van Riel, P. L., van der Meer, J. W. and Stalenhoef, A. F. 2007. The role of TNF-alpha in chronic inflammatory conditions, intermediary metabolism, and cardiovascular risk. J Lipid Res 48, 751-762.
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