Oregano (Origanum vulgare) is an aromatic herb belonging to the Lamiaceae family. This study evaluated the therapeutic effect of oregano essential oil (OEO) on clinical bovine mastitis caused by Staphylococcus (S.) aureus and/or Escherichia (E.) coli. Thirty-two quarters from 18 lactating cows with ...
Oregano (Origanum vulgare) is an aromatic herb belonging to the Lamiaceae family. This study evaluated the therapeutic effect of oregano essential oil (OEO) on clinical bovine mastitis caused by Staphylococcus (S.) aureus and/or Escherichia (E.) coli. Thirty-two quarters from 18 lactating cows with subclinical mastitis were selected from a dairy farm and were divided into 4 groups (8 quarters/group): negative control (CON), positive control (GEN) and two OEO-treated groups (OEO-1 and OEO-2). Each group was intramammarily treated with saline, 10 g/tube of gentamicin ointment, and single and double doses of 0.9 mL OEO ointment twice a day for three days, respectively. After the treatments, physical udder conditions were greatly improved in GEN and OEO groups. In CON group, somatic cell counts (SCCs), number of white blood cells (WBC) and bacteria were increased. However, in OEO groups, SCCs and number of WBC were significantly decreased and S. aureus and E. coli were not detected in milk as compared with those before the treatments as well as GEN group. These results suggest that OEO may be a useful alternative to antibiotics for the control of subclinical bovine mastitis caused by S. aureus and/or E. coli.
Oregano (Origanum vulgare) is an aromatic herb belonging to the Lamiaceae family. This study evaluated the therapeutic effect of oregano essential oil (OEO) on clinical bovine mastitis caused by Staphylococcus (S.) aureus and/or Escherichia (E.) coli. Thirty-two quarters from 18 lactating cows with subclinical mastitis were selected from a dairy farm and were divided into 4 groups (8 quarters/group): negative control (CON), positive control (GEN) and two OEO-treated groups (OEO-1 and OEO-2). Each group was intramammarily treated with saline, 10 g/tube of gentamicin ointment, and single and double doses of 0.9 mL OEO ointment twice a day for three days, respectively. After the treatments, physical udder conditions were greatly improved in GEN and OEO groups. In CON group, somatic cell counts (SCCs), number of white blood cells (WBC) and bacteria were increased. However, in OEO groups, SCCs and number of WBC were significantly decreased and S. aureus and E. coli were not detected in milk as compared with those before the treatments as well as GEN group. These results suggest that OEO may be a useful alternative to antibiotics for the control of subclinical bovine mastitis caused by S. aureus and/or E. coli.
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제안 방법
At the first time, this study carried out the application of OEO known as antibacterial activity to treat bacterial bovine mastitis and identified OEO as a potential candidate for the control of bovine mastitis.
CON was treated with saline twice a day for 3 days. GEN was treated with 10 g/tube of gentamicin ointment (Daesung Gentamicin Cream; Daesung Microbiological Labs, Korea), twice a day for 3 days. OEO-1 and OEO-2 had single and double doses of OEO ointment containing 0.
Change of somatic cell count before (□) and after (■) intramammary treatment with oregano essential oil (OEO) in cattle with mastitis. Negative control (CON) treated with saline; positive control (GEN) treated with 10 g/tube of gentamicin ointment twice a day for 3 days; single (OEO-1) and double (OEO2) doses of 0.9 mL OEO ointment twice a day for three days. Asterisks (**) indicate p < 0.
GEN was treated with 10 g/tube of gentamicin ointment (Daesung Gentamicin Cream; Daesung Microbiological Labs, Korea), twice a day for 3 days. OEO-1 and OEO-2 had single and double doses of OEO ointment containing 0.9 mL of OEO (Eco-Mast 10 mL/tube; Daehan New Pharm, Korea) infused into the inflamed quarters twice a day for 3 days, respectively.
대상 데이터
This study was conducted in a dairy farm located in the Changwon area of Gyeongsang province, Korea. Thirty-two quarters from 18 lactating cows with clinical mastitis that had not been responding to treatment for over one weeks, were selected for this study. Clinical mastitis was defined as having somatic cell counts (SCCs) greater than 2.
This study was conducted in a dairy farm located in the Changwon area of Gyeongsang province, Korea. Thirty-two quarters from 18 lactating cows with clinical mastitis that had not been responding to treatment for over one weeks, were selected for this study.
데이터처리
Data were analyzed using one-way analysis of variance (ANOVA), followed by Student’s two tailed t-test.
성능/효과
However, WBC numbers in GEN, OEO-1 and OEO-2 groups after each treatment for 3 days, were significantly decreased compared with those before the treatment (p < 0.05).
In conclusion, results from this study suggest that OEO may be a useful alternative to antibiotics for the control of bovine mastitis caused by S. aureus and/or E. coli.
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