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NTIS 바로가기Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.43 no.4, 2015년, pp.314 - 321
이창로 (명지대학교 생명과학정보학부) , 지원재 (국립생물자원관 유용자원분석과) , 배창환 (국립생물자원관 유용자원분석과) , 홍순광 (명지대학교 생명과학정보학부)
Agar-hydrolyzing bacteria were isolated from the coastal sea water of Jeju Island. One isolate, designated as S4, was selected for further study. The S4 cells were Gram-negative and rod-shaped with smooth beige surfaces and single polar flagellum. Cells were grown at
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핵심어 | 질문 | 논문에서 추출한 답변 |
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효율적으로 한천을 분해할 수 있는 한천분해효소 개발이 해양바이오메스 홍조류의 이용에 절대적으로 필요한 이유는 무엇인가 | (네오)아가로올리고당은 hydrolase에 의해서 최종적으로 3,6-anhydro-L-galactose와 D-galactose로 분해되는데, D-galactose는 chemical feedstock 또는 2차 발효를 통한 바이오 연료 생산 등에 사용될 수 있다. 3,6-anhydro-L-galactose는 미백 및 항염증 효과가 뛰어난 것으로 보고되었고[28], 2차 발효를 통한 바이오 연료 생산 등에도 사용될 수 있어 홍조류 바이오메스의 자원으로서 이용가치가 확대되고 있다. | |
한천의 구성성분은 무엇인가 | 한천(agar)은 홍조류 세포벽의 주요 구성 성분으로서 3-O-linked β-D-galactopyranose(G)와 4-O-linked α-L-1,3-anhydrogalactopyranose(LA), 또는 3-O-linked β-D-galactopyranose(G)와 4-O-linked α-L-galactopyranose-6-sulfate(L6S) 단위체로 구성되어 있다. 한천의 주요 성분인 agarose는 agarobiose(G-LA) 단위체가 반복되는 구조이며, porphyran은 porphyrobiose(G-L6S) 단위체의 반복 구조이다[13]. | |
한천의 효소적 분해공정에서 심각한 제한요소는 무엇인가 | 하지만 대부분의 경우 agarse의 효소 활성이 35−40℃ 범위에서 활성을 보이는 중온성 효소이다. 실제 한천의 효소적 분해공정에서 심각한 제한요소가 효소의 작용 온도이다. 한천은 상온에서 겔화 되는 특성이 있어 중온성 agarase를 사용할 경우 0. |
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