[국내논문]발효 황금 뿌리 추출물의 항균, 항산화 효과 및 메탄가스 저감 효과 In Vitro Analysis of Antibacterial, Antioxidant, and In Vitro Methane Mitigation Activities of Fermented Scutellaria baicalensis Georgi Extract원문보기
This study was conducted to investigate the antibacterial, antioxidant, and in vitro greenhouse gas mitigation activities of fermented Scutellaria baicalensis Georgi extract. Seven starter cultures were used, comprising four of lactic acid bacteria and three of Saccharomyces cerevisiae. Ten grams of...
This study was conducted to investigate the antibacterial, antioxidant, and in vitro greenhouse gas mitigation activities of fermented Scutellaria baicalensis Georgi extract. Seven starter cultures were used, comprising four of lactic acid bacteria and three of Saccharomyces cerevisiae. Ten grams of S. baicalensis Georgi powder was diluted in 90 mL autoclaved MRS broth. Each seed culture was inoculated with 3-10% (v/v) S. baicalensis Georgi MRS broth and incubated at $30^{\circ}C$ for 48 h. Among the starter cultures used, only Lactobacillus plantarum EJ43 could withstand the fermentation conditions. This fermentation broth was dried and extracted with ethanol to assess its antibacterial, antioxidant, and in vitro methane mitigation activities. The extract of S. baicalensis Georgi fermented by L. plantarum EJ43 (SBLp) showed higher antibacterial activity (bigger clear zone) compared to the unfermented S. baicalensis Georgi extract (SB0). SBLp also presented 1.2 folds higher antioxidant activity than SB0. During in vitro rumen fermentation, SBLp showed reduction in methane production compared to SB0 or the control. In conclusion, fermentation by L. plantarum EJ43 may enhance antibacterial and antioxidant activities of S. baicalensis Georgi and decrease enteric methane production.
This study was conducted to investigate the antibacterial, antioxidant, and in vitro greenhouse gas mitigation activities of fermented Scutellaria baicalensis Georgi extract. Seven starter cultures were used, comprising four of lactic acid bacteria and three of Saccharomyces cerevisiae. Ten grams of S. baicalensis Georgi powder was diluted in 90 mL autoclaved MRS broth. Each seed culture was inoculated with 3-10% (v/v) S. baicalensis Georgi MRS broth and incubated at $30^{\circ}C$ for 48 h. Among the starter cultures used, only Lactobacillus plantarum EJ43 could withstand the fermentation conditions. This fermentation broth was dried and extracted with ethanol to assess its antibacterial, antioxidant, and in vitro methane mitigation activities. The extract of S. baicalensis Georgi fermented by L. plantarum EJ43 (SBLp) showed higher antibacterial activity (bigger clear zone) compared to the unfermented S. baicalensis Georgi extract (SB0). SBLp also presented 1.2 folds higher antioxidant activity than SB0. During in vitro rumen fermentation, SBLp showed reduction in methane production compared to SB0 or the control. In conclusion, fermentation by L. plantarum EJ43 may enhance antibacterial and antioxidant activities of S. baicalensis Georgi and decrease enteric methane production.
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제안 방법
Antioxidant activity was examined using DPPH (2,2-di (4-tert-octylphenyl)-1-picrylhydrazyl, Sigma, USA) to measure the free radical scavenging activity. Diluted extract (0.
baicalensis Georgi extract was added to the mixture, the bottle was capped and incubated at 39℃, in triplicates. Gas production was measured at 3, 6, 9, 12, and 24 h using a glass syringe (Sercrim Labtech Co. Ltd., South Korea). The produced gas was collected in an aluminum bag to determine the concentration of methane and carbon dioxide.
Further differences between means were subjected to Duncan’s multiple range test, and significant effect of treatment were declared at P < 0.05, with tendencies at 0.05 < P < 0.10.
In this study, three different elution systems were applied to separate a compound in the extract relevant to antioxidant activity. To identify the constituent related to the antioxidant activity, TLC plate was stained with DPPH solution.
대상 데이터
S. baicalensis was purchased from a medicinal plant market (Kyungdong market) in Seoul, South Korea and was ground to 2 mm size by a cutter miller (HR2860, Phillips, South Korea). Ten grams of ground S.
, Germany). VFA standard solution (Sigma Aldrich, USA) and BR-Wax fame (BR87503, Germany) column were used. Injector and detector (flame ionized detector) were aligned to 250℃, whereas oven temperature was 100℃.
The bacterial growth in the MRS medium containing S. baicalensis Georgi (10%, w/v) was screened. Viability was only observed in S.
이론/모형
Antibacterial activity of the extract was determined using the agar well diffusion method. For pathogenic bacteria, Staphylococcus aureus (wild type), Listeria monocytogenes KACC0550, Salmonella gallinarum ATCC9184, and Mannheimia haemolytica (wild type) were used, and they were grown in Luria-Bertani (LB) broth (Difco, Maryland, USA) at 37℃ in a shaking incubator for 24 h.
Dry matter digestibility (DMD) was measured at 0 and 24 h, using a nylon bag to transfer any residues from the incubation bottle, which was washed with tap water until the water ran clear. Volatile fatty acid (VFA) concentration was determined according to the method by Erwin et al. (1961). A 1 mL aliquot of sample pre-treated with 0.
성능/효과
plantarum EJ43, with cell counts being approximately 107 CFU/mL. Therefore, in this experiment, it was concluded that L. plantarum EJ43 could ferment S. baicalensis Georgi root, and further extraction was performed only with this culture.
2B). As a result, it was found that TLC-DPPH with CEF and BEA could be used for the detection of newly synthesized and/or newly altered compounds, and fermentation with L. plantarum EJ43 could change unknown compounds in S. baicalensis that may have potential to antioxidant activity.
Total VFA production numerically decreased when the extract of S. baicalensis was supple- mented, although there was no statistical difference among the treatments (Table 2). This result did not match with that of DMD, which was highest for SB0.
후속연구
The total gas production by SB0 and SBLp was lower than that by CON (Table 3). However, SBLp also lowered methane production, indicating that the extract of this particular plant after fermentation may be used for methane control in the diet of ruminants, although further studies are warranted.
Therefore, it may be suggested that this extract can be potentially developed as an alternative feed additive, to replace antibiotics in animal industry. Further research is necessary to identify the compound observed during the TLC analysis, and in vivo experiments are required to examine the efficacy of the extract.
baicalensis Georgi and control. Further studies are warranted to identify unknown antioxidant compounds in the extract of fermented S. baicalensis, and in vivo evaluation is necessary to confirm the effects of S. baicalensis extract.
참고문헌 (20)
Bruno, M. E. C. and T. J. Montville. 1993. Common mechanistic action of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 59: 3003-3010.
Erwin, E. S., G. J. Marco, and E. M. Emery. 1961. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44: 1768-1771.
Gao, Z., K. Huang, and H. Xu. 2001. Protective effects of flavonoids in the roots of scutellaria baicalensis georgi against hydrogen peroxide-induced oxidative stress in HSSY5Y cells. Pharmacol. Res. 43: 173-178.
Gao, Z., K. Huang, X. Yang, and H. Xu. 1999. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim. Biophys. Acta. 1472: 643-650.
Guo, A. J. Y., R. C. Y. Choi, A. W. H. Cheung, V. P. Chen, S. L. Xu, T. T. X. Dong, J. J. Chen, and K. W. K. Tsim. 2011. Baicalin, a flavone, induces the differentiation of cultured osteoblasts: an action via the Wnt/ ${\beta}$ -catenin signaling pathway. J. Biol. Chem. 286: 27882-27893.
Hungate, R. E., J. Reichl, and R. Prins. 1971. Parameters of fermentation in a continuously fed sheep: evidence of a microbial rumination pool. Appl. Microbiol. 22: 1104-1113.
Hungate, R. E., W. Smith, T. Bauchop, I. Yu, and J. C. Rabinowitz. 1970. Formate as an Intermediate in the Bovine Rumen Fermentation. J. Bacteriol. 102: 389-397.
Johnson, K. A. and D. E. Johnson. 1995. Methane emissions from cattle. J. Anim. Sci. 73: 2483-2492.
Landers, T. F., B. Cohen, T. E. Wittum, and E. L. Larson. 2012. A review of antibiotic use in food animals: Perspective, policy, and potential. Public Health Rep. 127: 4-22.
Masoko, P. and J. N. Eloff. 2007. Screening of twenty-four South African Combretum and six Terminalia species (Combretaceae) for antioxidant activities. Afr. J. Tradit. Complement. Altern. Med. 4: 231-239.
Mathew, A. G., R. Cissell, and S. Liamthong. 2007. Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production. Foodborne. Pathog. Dis. 4: 115-33.
Mukherjee, S. and A. Ramesh. 2015. Bacteriocin-producing strains of Lactobacillus plantarum inhibit adhesion of Staphylococcus aureus to extracellular matrix: quantitative insight and implications in antibacterial therapy. J. Med. Microbiol. 64: 1514-1526.
Nightingale, K. K., Y. H. Schukken, C. R. Nightingale, E. D. Fortes, A. J. Ho, Z. Her, Y. T. Grohn, P. L. McDonough, and M. Wiedmann. 2004. Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl. Environ. Microbiol. 70: 4458-4467.
Wilson, A. R., D. Sigee, and H. A. S. Epton. 2005. Anti-bacterial activity of Lactobacillus plantarum strain SK1 against Listeria monocytogenes is due to lactic acid production. J. Appl. Microbiol. 99: 1516-1522.
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