본 연구는 구강환경요인을 살펴보고, 구강환경이 구강세균 검출에 미치는 위험요인 알아보고자 시행하였다. 연구기간은 2015년 2월 15일~ 2월 28일까지 성인 근로자 60명을 대상으로 조사하였다. 연구변수로는 안정시, 자극시 타액분비율, 타액완충능, 타액 pH, 설배와 설하 구강건조도, 구취 설태량을 측정하였다. 구강세균의 검출 유무를 확인하기 위하여 안정시 타액분비율의 gDNA를 추출하여 균을 검출하였다. 그 결과, S.mutans균은 흡연자 15배, 자극시, 안정시 타액분비율 1.3~1.6배 위험도가 더 높았으며, P.intermedia는 흡연자 13배, 설하 구강건조도 4.3배, 설태량 4배 TM7은 설하 구강건조도 5.5배 더 높은 위험도를 나타냈다. 구강 내 세균을 줄이기 위해서는 타액분비율을 촉진시키는 습관을 형성하고, 금연교육을 시행함이 중요하다고 본다. 또한 구강위생이 깨끗하고 관리가 잘되는 성인에서는 구강세균이 많이 줄어들고, 구강질환을 좀 더 예방할 수 있을 것이라 기대된다.
본 연구는 구강환경요인을 살펴보고, 구강환경이 구강세균 검출에 미치는 위험요인 알아보고자 시행하였다. 연구기간은 2015년 2월 15일~ 2월 28일까지 성인 근로자 60명을 대상으로 조사하였다. 연구변수로는 안정시, 자극시 타액분비율, 타액완충능, 타액 pH, 설배와 설하 구강건조도, 구취 설태량을 측정하였다. 구강세균의 검출 유무를 확인하기 위하여 안정시 타액분비율의 gDNA를 추출하여 균을 검출하였다. 그 결과, S.mutans균은 흡연자 15배, 자극시, 안정시 타액분비율 1.3~1.6배 위험도가 더 높았으며, P.intermedia는 흡연자 13배, 설하 구강건조도 4.3배, 설태량 4배 TM7은 설하 구강건조도 5.5배 더 높은 위험도를 나타냈다. 구강 내 세균을 줄이기 위해서는 타액분비율을 촉진시키는 습관을 형성하고, 금연교육을 시행함이 중요하다고 본다. 또한 구강위생이 깨끗하고 관리가 잘되는 성인에서는 구강세균이 많이 줄어들고, 구강질환을 좀 더 예방할 수 있을 것이라 기대된다.
This research examined the oral environmental factors to identify the risk factors for oral bacteria detection. This study comprised of 60 office workers aged between 20 and 65 years, and was performed from January 15 to February 28, 2015. The study variables measured were the stimulated and unstimu...
This research examined the oral environmental factors to identify the risk factors for oral bacteria detection. This study comprised of 60 office workers aged between 20 and 65 years, and was performed from January 15 to February 28, 2015. The study variables measured were the stimulated and unstimulated salivary flow rates, salivary buffering, saliva pH, dry mouth at the dorsum of the tongue and the sublingual region, halitosis, and the degree of tongue-coating as oral environmental factors. To identify the presence of oral bacteria, pathogens were detected by extracting the gDNA of the resting salivary flow rate. The risk of S.mutans detection was 15 times higher with smokers, 1.3~1.6 times higher when the resting or stimulated salivary flow rate was reduced by 1 mm. The risk of P.intermedia detection was 13 times higher in smokers, 4.3 times higher as the severity of oral dryness was lowered, and 4 times higher for adults with a tongue coating than those without. In addition, the risk of detecting TM7 was 5.5 times higher as sublingual dryness was decreased by 1mm. The oral bacterial count will be reduced considerably by smoking cessation education and habits that facilitate a salivary flow rate. Furthermore, adults with good and well-managed dental hygiene are anticipated to have less oral bacteria and fewer dental diseases.
This research examined the oral environmental factors to identify the risk factors for oral bacteria detection. This study comprised of 60 office workers aged between 20 and 65 years, and was performed from January 15 to February 28, 2015. The study variables measured were the stimulated and unstimulated salivary flow rates, salivary buffering, saliva pH, dry mouth at the dorsum of the tongue and the sublingual region, halitosis, and the degree of tongue-coating as oral environmental factors. To identify the presence of oral bacteria, pathogens were detected by extracting the gDNA of the resting salivary flow rate. The risk of S.mutans detection was 15 times higher with smokers, 1.3~1.6 times higher when the resting or stimulated salivary flow rate was reduced by 1 mm. The risk of P.intermedia detection was 13 times higher in smokers, 4.3 times higher as the severity of oral dryness was lowered, and 4 times higher for adults with a tongue coating than those without. In addition, the risk of detecting TM7 was 5.5 times higher as sublingual dryness was decreased by 1mm. The oral bacterial count will be reduced considerably by smoking cessation education and habits that facilitate a salivary flow rate. Furthermore, adults with good and well-managed dental hygiene are anticipated to have less oral bacteria and fewer dental diseases.
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문제 정의
Therefore, this study examined oral environmental factors and the presence of oral bacteria in adult workers. This investigation aims to establish a reference base to diagnose and prevent oral diseases by identifying risk factors of oral bacteria.
Therefore, this study examined oral environmental factors and the presence of oral bacteria in adult workers. This investigation aims to establish a reference base to diagnose and prevent oral diseases by identifying risk factors of oral bacteria.
This study aimed to examine oral environmental factors and identify risk factors affecting oral bacteria detection in oral environment.
제안 방법
To identify bacteria species, we measured unstimulated and stimulated SFRs. To identify oral environmental factors salivary buffering capacity, saliva pH, dryness at the dorsum of the tongue and the sublingual region, halitosis, and the degree of tongue-coating.
Species-specific PCR primers were used to detect S.mutans ATCC25175, P.intermedia ATCC25611, and TM7 phylum designed in this study. To obtain the optimum annealing temperature of any primer pair, denaturation and extension were consistently carried out at 95ºC for 30sec and 72ºC for 1 min.
PCR was performed at 59-72ºC for 1 min for annealing. For the verification of manufactured PCR primers, procedures were done on a PTC-100 thermocycler (MJ Research Ins., Watertown, MA, USA) using AccuPower PCR Premix (Bbioneer Corp., Daejeon, Korea). To make a 25ul of PCR mixed solution, a 5uM of forward and reverse primers each and a 1ug of genomic DNA of each strain were added.
A chi-square test was used to verify the association of oral bacteria detection with drinking, smoking, systemic diseases, oral environmental factors (stimulated or unstimulated SFR, salivary buffering capacity, pH, halitosis, and tongue-coating). Moreover, a logistic regression analysis was performed to verify the risk factors of oral bacteria according to oral environmental factors.
대상 데이터
This study comprised 60 office workers aged between 20 and 65 years, and performed from January 15 to February 28, 2015. All subjects consented and were fully informed about research procedures.
This study was conducted after gaining Institutional review board(IRB) approval (BUIRB-201410-HR-0011). In this research, 20 men (33.3%) and 40 women (66.7%) were enrolled as subjects, and their mean age was 33.8 years. There were 37 non-smokers (61.
The results of detected oral bacteria are shown in Figure 2. There were 38 subjects with S.mutans, 46 with P.intermedia, 9 with TM7, 32 with S.mutans and P.intermedia, 6 with S.mutans and TM7, 9 with P.intermedia and TM7, 6 with all three bacteria species, and 8 without any bacteria.
성능/효과
First, The results of this study show that S.mutans, an etiological agent of tooth decay, is dominantly found in smokers. The risk of S.
Second, According to the finding of this study, the risk of S.mutans detection is identified to be 1.3-1.6 times higher in adults with a lower SFR in comparison to the healthy individuals. The risks of P.
Third, The risk of P.intermedia detection has been reported to be about 4 times higher in adults with a greater amount of tongue-coating, indicating the importance of tongue-coating management. In fact, there are many reports on amount of tongue-coating and its high relationship with halitosis.
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