[논문]반응표면분석법을 이용한 용아초 기능성 성분의 마이크로웨이브 최적 추출조건 예측

박종진; 이원영

doi:10.7783/kjmcs.2016.24.4.263

반응표면분석법을 이용한 용아초 기능성 성분의 마이크로웨이브 최적 추출조건 예측
Prediction of Optimal Microwave Extraction Conditions for Functional Compounds from Agrimonia pilosa Ledeb Using Response Surface Methodology 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.24 no.4, 2016년, pp.263 - 270

박종진 (경북대학교 식품공학부 식품응용공학과) , 이원영 (경북대학교 식품공학부 식품응용공학과)

Abstract ▼ AI-Helper

Background: In this study, microwave extraction was used, which is an effective method to extract useful bioactive substances as it requires low quantities of solvent and short time periods. The aim of this study was to determine the optimal extraction conditions for Agrimonia pilosa Ledeb. Methods and Results: The independent variables were ethanol concentration, microwave power, and extraction time, each of which had five levels. The dependent variables were total polyphenol and total flavonoid content, and DPPH radical scavenging activity. To determine the optimal extraction conditions for bioactive compounds, a response surface methodology was employed. Contour maps were generated from polynomial equations. The optimal conditions were then assumed by superimposing these contour maps. Based on the resulting graph, the optimal microwave extraction conditions for Agrimonia pilosa Ledeb were determined as 42 - 48% ethanol concentration, 240 - 280W microwave power, and 13 - 20 min of extraction time. Conclusions: Ethanol concentration had a significant effect on microwave extraction, in terms of total polyphenol and total flavonoid content, as well as DPPH radical scavenging activity. Microwave power and extraction time influenced the total polyphenol content, but not the total flavonoid content or the DPPH radical scavenging activity.

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문제 정의

The aim of this study is to determine the optimum extraction condition of DPPH radical scavenging activity (DPPH) and functional ingredients, including total polyphenol content (TPC) and total flavonoid content (TFC) by using microwave-assisted extraction and response surface methodology. Those results will be fundamental data to set microwave extraction condition of A.

가설 설정

In this study, aqueous ethanol shows a higher TPC than pure ethanol and water. This result indicated that aqueous ethanol reacts more effectively with microwave energy to produce a high TPC.

제안 방법

The experimental data were expressed to statistical three dimension surface and contour plots. The contour maps were overlapped to indicate optimal conditions.
Through single-factor experiments and central composite design (CCD), ethanol concentration (0, 25, 50, 75 and 100%), microwave power (60, 120, 180, 240 and 300 W), and extraction time (4, 8, 12, 16 and 20 min) were selected as suitable conditions for extraction and were encoded under five levels (−2, −1, 0, +1 and +2) (Table 1).

대상 데이터

The Agrimonia pilosa Ledeb used in this study was harvested from the Gyeongsang province in Korea. The dried sample was purchased in the market.
A microdigest machine (Soxwave 100, Prolabo, Fontenay, France) was used to extract total polyphenol, total flavonoid, and antioxidants. The device operates at a frequency of 2,450 MHz and is equipped with a reflux condenser. A total of 50 ㎖ of ethanol was mixed with 1 g of sample powder and used to fill an extraction vessel.
To select proper solvent for extraction, four different solvents including distilled water, ethanol, acetone and ethyl acetate were used. Among them, ethanol and distilled water extracts show higher contents of functional compounds and DPPH radical scavenging activity than acetone and ethyl acetate.

이론/모형

DPPH radical scavenging activity was assayed by using a modified version of the DPPH method by Blois (1958). To make DPPH solution, 0.
The total flavonoid content was analyzed by using aluminum colorimetric assay (Teng et al., 2009). Approximately 70 ㎕ of extract, which was diluted two times with distilled water, was mixed with 430 ㎕ of distilled water, 50 ㎕ of 5% NaNO₂ and 50 ㎕ of 10% Al(NO₃)₃9H₂O.

성능/효과

In this study, extraction of TFC was enhanced in the range of ethanol concentration from 45 to 55% with increase of extraction time. But TFC was decreased more than 20 min of extraction time.
The experimental values for TPC, TFC, and DPPH were 35.15 ± 1.58 ㎎ · GAE/g, 103.61 ± 4.36 ㎎ · RE/g, and 68.74 ± 1.40%, respectively (Table 5).

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