혈관신생과정은 종양 형성과 이동에 필수적인 과정으로 촉진제와 저해제에 의하여 조절되며, 이러한 혈관신생과정의 저해는 새로운 항암치료 기법으로 이용하고 있다. 최근, 한약재와 식료품으로부터 추출한 천연물을 새로운 치료 물질로 널리 이용하고 있으며, 실제 in vitro 뿐만 아니라 in vivo 상에서도 항암 효과가 나타나는 것을 확인하였다. 그 중 도라지는 아시아에서 한약재와 식료품으로 오랫동안 사용 되어왔다. 본 연구에서는 도라지 추출물이 in vitro 상에서 인간 제대 정맥 내피 세포의 혈관신생을 억제하는 효과에 대해 조사하였다. 도라지 추출물은 세포독성 없이, 혈관 형성 및 이동, 침윤 현상을 모두 억제하는 효과를 보였다. 특히, 세포 이동은 80% 정도 감소 시켰으며 침윤 현상이 거의 나타나지 않는 것을 확인하였다. 이러한 결과를 토대로 도라지 추출물은 혈관신생 억제제로 이용할 수 있으며, 더 나아가 항암제로 개발될 수 있을 것이라 사료된다.
혈관신생과정은 종양 형성과 이동에 필수적인 과정으로 촉진제와 저해제에 의하여 조절되며, 이러한 혈관신생과정의 저해는 새로운 항암치료 기법으로 이용하고 있다. 최근, 한약재와 식료품으로부터 추출한 천연물을 새로운 치료 물질로 널리 이용하고 있으며, 실제 in vitro 뿐만 아니라 in vivo 상에서도 항암 효과가 나타나는 것을 확인하였다. 그 중 도라지는 아시아에서 한약재와 식료품으로 오랫동안 사용 되어왔다. 본 연구에서는 도라지 추출물이 in vitro 상에서 인간 제대 정맥 내피 세포의 혈관신생을 억제하는 효과에 대해 조사하였다. 도라지 추출물은 세포독성 없이, 혈관 형성 및 이동, 침윤 현상을 모두 억제하는 효과를 보였다. 특히, 세포 이동은 80% 정도 감소 시켰으며 침윤 현상이 거의 나타나지 않는 것을 확인하였다. 이러한 결과를 토대로 도라지 추출물은 혈관신생 억제제로 이용할 수 있으며, 더 나아가 항암제로 개발될 수 있을 것이라 사료된다.
Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful sou...
Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful source of novel therapeutic compounds. The balloon-flower has long been used as a traditional medicinal material and food in Asia. In this study, we investigated whether extract of balloon-flower would inhibit in vitro angiogenesis and vascular-like network formation in human umbilical vein endothelial cells (HUVECs). The extract of Balloon-flower did not affect the viability of HUVECs. However, treatment with the Balloon-flower extract suppressed tube formation of HUVECs. In addition, after treatment with the Balloon-flower extract, cell migration decreased about 80%, and cell invasion was almost completely inhibited. Taken together, these results suggest that extract of Balloon-flower may have potential as an angiogenic inhibitor and that it could be developed as an anticancer agent.
Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful source of novel therapeutic compounds. The balloon-flower has long been used as a traditional medicinal material and food in Asia. In this study, we investigated whether extract of balloon-flower would inhibit in vitro angiogenesis and vascular-like network formation in human umbilical vein endothelial cells (HUVECs). The extract of Balloon-flower did not affect the viability of HUVECs. However, treatment with the Balloon-flower extract suppressed tube formation of HUVECs. In addition, after treatment with the Balloon-flower extract, cell migration decreased about 80%, and cell invasion was almost completely inhibited. Taken together, these results suggest that extract of Balloon-flower may have potential as an angiogenic inhibitor and that it could be developed as an anticancer agent.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
대상 데이터
Data are presented as means ± standard deviation (SD). All experiments were performed in triplicate.
Grain-alcoholic extracts derived from biomass, dried root and leaf of Balloon-flower, has been prepared upon cold sonication process at temperature of 20℃. Biomass was purchased from Kyung-dong medicinal herb market (Seoul, Korea). Biomass has been powdered then, mixed with 70% alcoholic solution in pure water before processing.
Basic fibroblast growth factor (bFGF) and heparin were obtained from Pepprotech (Gaithersbeg, USA). M199, Fetal Bovine Serum (FBS), penicillin and streptomycin were purchased from JBI (Daegu, Korea). Matrigel was purchased from Collaborative Biomedical Products (Bedford, MA.
데이터처리
Statistical comparison was performed using Student’s t-test.
이론/모형
Extracts of Balloon-flower inhibits the vascular network formation. (A) The cytotoxic effect of Balloon-flower extract on the HUVECs was determined using the MTT assay. The treatment for 24 hr did not affect the cell viability of HUVEC cells in the tested concentrations.
참고문헌 (23)
Adriana, B. R., Rafael, M. L. and Gilberto, S. 2001. Natural products in anticancer therapy. Curr. Opi. Phamacol. 1, 364-369.
Chen, X., Bao, J., Guo, J., Ding, Q., Lu, J., Huang, M. and Wang, Y. 2012. Biological activities and potential molecular targets of cucurbitacins: a focus on cancer. Anticancer Drugs 23, 777-787.
Efferth, T., Li, P. C., Konkimalla, V. S. and Kaina, B. 2007. From traditional Chinese medicine to rational cancer therapy. Trends Mol. Med. 13, 353-361.
Huang, M., Lu, J. J., Huang, M. Q., Bao, J. L., Chen, X. P. and Wang, Y. T. 2012. Terpenoids: natural products for cancer therapy. Expert. Opin. Investig. Drugs 21, 1801-1818.
Lee, J. S., Choi, M. S., Seo, K. I., Lee, H. I., Lee, J. H., Kim, M. J. and Lee, M. K. 2014. Platycodi radix saponin inhibits glucosidase in vitro and modulates hepatic glucose-regulating enzyme activities in C57BL/KsJ -db/db mice. Arch. Pharm. Res. 37, 773-782.
Lee, W. H., Gam, C. O., Ku, S. K. and Choi, S. H. 2011. Single oral dose toxicity test of platycodin d, a saponin from platycodin radix in mice. Toxicol. Res. 27, 217-224.
Meng, Z., Garrett, C. R., Shen, Y., Liu, L., Yang, P., Huo, Y., Zhao, Q., Spelman, A. R., Ng, C. S., Chang, D. Z. and Cohen, L. 2012. Prospective randomized evaluation of traditional Chinese medicine combined with chemotherapy: a randomised phase II study of wild toad extract plus gemcitabine in patients with advanced pancreatic adenocarcinomas. Br. J. Cancer 107, 411-416.
Morishita, S., Shoji, M., Oguni, Y., Ito, C., Higuchi, M. and Sakanashi, M. 1992. Pharmacological actions of "kyushin," a drug containing toad venom: cardio-tonic and arrhythmogenic effects, and excitatory effect on respiration. Am. J. Chin. Med. 20, 245-256.
Naldini, A. and Carraro, F. 2005. Role of inflammatory mediators in angiogenesis. Curr. Drug Targets Inflam. Allergy 4, 3-8.
Neychev, V. K., Nikolova, E., Zhelev, N. and Mitev, V. I. 2007. Saponins from Tribulusterrestris L. are less toxic for normal human fibroblasts than for many cancer lines: Influence on apoptosis and proliferation. Exp. Biol. Med. 232, 126-133.
Park, D. I., Lee, J. H., Moon, S. K., Kim, C. H., Lee, Y. T., Cheong, J., Choi, B. T. and Choi, Y. H. 2005. Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells. Pharmacol. Res. 51, 437-443.
Qi, F., Li, A., Zhao, L., Xu, H., Inagaki, Y., Wang, D., Cui, X., Gao, B., Kokudo, N., Nakata, M. and Tang, W. 2010. Cinobufacini, an aqueous extract from Bufo bufo gargarizans Cantor, induces apoptosis through a mitochondria-mediated pathway in human hepatocellular carcinoma cells. J. Ethnopharmacol. 128, 654-661.
Qin, H., Du, X., Zhang, Y. and Wang, R. 2014. Platycodin D, a triterpenoid saponin from Platycodon grandiflorum, induces G2/M arrest and apoptosis in human hepatoma HepG2 cells by modulating the PI3K/Akt pathway. Tumour Biol. 35, 1267-1274.
Siddiqui, F. A., Desai, H., Siddiqui, T. F. and Francis, J. L. 2002. Hemoglobin induces the expression and secretion of vascular endothelial growth factor from human malignant cells. Hematol. J. 3, 264-270.
Zhang, L., Wang, Y., Yang, D., Zhang, C., Zhang, N., Li, M. and Liu, Y. 2015. Platycodon grandifloras-An ethnopharmacological, phytochemical and pharmacological review. J. Ethnopharmacol. 164, 147-161.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.