[논문]Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors

Oh, Jihae; Lee, Chiwoo; Kaang, Bong-Kiun

doi:10.4196/kjpp.2019.23.4.237

Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors 원문보기 논문타임라인

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.23 no.4, 2019년, pp.237 - 249

Oh, Jihae (School of Biological Sciences, Seoul National University) , Lee, Chiwoo (School of Biological Sciences, Seoul National University) , Kaang, Bong-Kiun (School of Biological Sciences, Seoul National University)

Abstract ▼ AI-Helper

Confirming the direct link between neural circuit activity and animal behavior has been a principal aim of neuroscience. The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. These GECI-based signals can be acquired by several tools including two-photon microscopy and microendoscopy for precise or wide imaging at cellular to synaptic levels. In addition, the images from GECI signals can be analyzed with open source codes including constrained non-negative matrix factorization for endoscopy data (CNMF_E) and miniscope 1-photon-based calcium imaging signal extraction pipeline (MIN1PIPE), and considering parameters of the imaged brain regions (e.g., diameter or shape of soma or the resolution of recorded images), the real-time activity of each cell can be acquired and linked with animal behaviors. As a result, GECI signal analysis can be a powerful tool for revealing the functions of neuronal circuits related to specific behaviors.

주제어

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문제 정의

In this paper, we reviewed the overall properties of GECIs, from calcium ions/indicators to image analysis. GECIs enable researchers to observe cells with behavior in real time, providing many neural circuit research advantages.
In this review, we discuss the developmental history as well as the pros and cons of ion indicators, focusing on genetically encoded calcium indicators (GECIs). Along with GECI properties, we discuss the imaging methods and corresponding GECI signal analysis.
Along with GECI properties, we discuss the imaging methods and corresponding GECI signal analysis. This review aims to offer an overview of the biophysical properties and limitations of the different GECI variants and to provide perspective to GECI users for choosing the best-suited variants.

참고문헌 (125)
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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
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