[논문]보성녹차 감압증류추출물의 주름개선, 미백 효능과 열수추출물의 항산화, 항균효과

이광원; 홍정현; 정선화; 김영균; 박신성; 박수인; 신문삼

doi:10.17703/jcct.2021.7.4.845

보성녹차 감압증류추출물의 주름개선, 미백 효능과 열수추출물의 항산화, 항균효과
Wrinkle Improvement, Whitening Effect of Boseong Camellia sinensis Vaccum Distilled Extract and Antioxidant, Antibacterial Efficacy of its Hydrothermal Extract 원문보기

Journal of the convergence on culture technology : JCCT = 문화기술의 융합, v.7 no.4, 2021년, pp.845 - 855

이광원 (을지대학교 시니어헬스케어학과) , 홍정현 (스킨룸(주)) , 정선화 (티아) , 김영균 ((주)차연) , 박신성 (을지대학교 미용화장품과학과) , 박수인 (을지대학교 시니어헬스케어학과) , 신문삼 (을지대학교 미용화장품과학과)

초록
AI-Helper

본 연구는 보성에서 재배된 녹차잎을 감압증류와 열수추출법으로 추출하여 항산화, 항주름, 미백, 항균 in vitro 실험과 HPLC 분석을 수행하였다. 보성녹차 감압증류추출물은 주름개선(Elastase 활성 저해 실험)과, 미백(Mushroom tyrosinase 활성 저해 실험) 효능에서 우수한 결과를 나타내었으며, 열수추출물보다 낮은 농도에서 IC50 값이 측정되었다. 보성녹차 열수추출물은 항산화 시험(DPPH, ABTS+ radical 소거능, SOD 유사활성능, 총 폴리페놀 함량)에서 우수한 결과를 나타내었다. 항균 실험에서는 보성녹차 열수추출물에 의해 Staphylococcus aureus, Cutibacterium acnes 균주에 대하여 Clear zone이 형성되었다. HPLC 분석 결과로, EGCG와 Caffeine이 열수추출물에서 40.29 mg/g, 9.94 mg/g 검출되었고, 감압증류추출물에서는 Caffeine이 3.4 mg/g 검출되었다. 따라서 보성녹차 감압증류추출물은 주름개선과 미백효과가 탁월하며, 보성녹차 열수추출물은 항산화, 항균효과가 우수함을 확인하였다.

Abstract ▼ AI-Helper

In this study, Camellia sinensis Leaf cultivated in Boseong was extracted by vacuum distillation and hydrothermal extraction methods, and we performed in vitro experiments such as antioxidant, anti-wrinkle, whitening, and antibacterial activity test and HPLC analysis. Wrinkle improvement (elastase inhibition assay) and whitening effect (mushroom tyrosinase inhibition assay) of Boseong vacuum distilled extract have excellent results and IC50 values of vacuum distillation extract was measured at a lower concentration than that of the hydrothermal extract. The antioxidant activity of the Boseong hydrothermal extract was excellently measured in DPPH radical and ABTS+ radical scavenging activity, SOD-like activity, and total polyphenol content. In the antibacterial experiment, the Boseong hydrothermal extract formed a clear zone for the Staphylococcus aureus and Cutibacterium acnes strain. As a result of HPLC analysis, EGCG and caffeine in the hydrothermal extract were 40.29 mg/g, 9.94 mg/g respectively, and caffeine in the vacuum distilled extract was 3.40 mg/g. Therefore, we examined that Boseong Camellia sinensis vacuum distilled extract has excellent anti-wrinkle and whitening effect, and its hydrothermal extract has good antioxidant and antibacterial efficacy.

주제어

표/그림 (9)

그림 그림 1. 보성녹차 추출물의 DPPH radical 소거능(%) Figure 1. DPPH radical scavenging activity of CSV, CSW Positive control: L-ascorbic acid 66.40 ± 0.21% (radical scavenging activity) at a concentration of 10 μg/mL, **P<0.01, ***P<0.001 vs control, CTL: control, sample untreated group, PC: Positive control, CSV: Camellia sinensis leaf vacuum distillation extract, CSW: Camellia sinensis leaf hydrothermal extract.
표 표 1. 보성녹차 추출물의 수율 Table 1. Yield of CSV, CSW
그림 그림 2. 보성녹차 추출물의 ABTS+ radical 소거능(%) Figure 2. ABTS+ radical scavenging activity of CSV, CSW. Positive control: L-ascorbic acid 66.45 ± 0.16% (radical scavenging activity) at a concentration of 10 μg/mL, ***P<0.001 vs control, CTL: Control, sample untreated group, PC: Positive control, CSV: Camellia sinensis leaf vacuum distillation extract, CSW: Camellia sinensis leaf hydrothermal extract.
그림 그림 3. 보성녹차 추출물의 SOD 유사활성능(%) Figure 3. SOD-like activity of CSV, CSW. Positive control: L-ascorbic acid 52.03 ± 1.80% (SOD-like activity) at a concentration of 20 μg/mL, **P<0.01, ***P<0.001 vs control, CTL: Control, sample untreated group, PC: Positive control, CSV: Camellia sinensis leaf vacuum distillation extract, CSW: Camellia sinensis leaf hydrothermal extract.
표 표 2. 보성녹차 감압증류추출물, 열수추출물에 함유된 총 폴리페놀 함량 Table 2. Total polyphenols of vacuum distillation and hydrothermal extracts from Camellia sinensis leaf
그림 그림 4. 보성녹차 추출물의 Elastase 활성 저해능(%) Figure 4. Elastase inhibitory activity of CSV, CSW Positive control: N-Methoxysuccinyl-(Ala)2-Pro-Val-chlorome thyl ketone 89.56 ± 1.64% (elastase inhibitory activity) at a concentration of 10 μg/mL, ***P<0.001 vs control, CTL: Control, sample untreated group, PC: Positive control, CSV: Camellia sinensis leaf vacuum distillation extract, CSW: Camellia sinensis leaf hydrothermal extract.
그림 그림 5. 보성녹차 추출물의 Tyrosinase 활성 저해능(%) Figure 5. Tyrosinase inhibitory activity of CSV, CSW. Positive control: Kojic acid 31.91 ± 0.19% (tyrosinase inhibitory activity) at a concentration of 10 μg/mL, ***P<0.001 vs control, CTL: Control, sample untreated group, PC: Positive control, CSV: Camellia sinensis leaf vacuum distillation extract, CSW: Camellia sinensis leaf hydrothermal extract.
표 표 3. 보성녹차 추출물의 clear zone 지름 측정 결과 Table 3. The result of clear zone diameter of CSV, CSW
표 표 4. 보성녹차의 추출법 따른 ECGC, Caffeine 분석 결과 Table 4. The analysis result of the contents of EGCG, caffeine containing Camellia sinensis leaf extracts from Boseong according to extraction method

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