[논문]Silymarin과 작약감초탕 병용투여의 C57BL/6 마우스 간조직 지질축적 및 염증 억제효과

최정원; 조수정; 신미래; 박해진

doi:10.6116/kjh.2022.37.5.17.

Silymarin과 작약감초탕 병용투여의 C57BL/6 마우스 간조직 지질축적 및 염증 억제효과
Combined Treatment of Silymarin and Jakyakgamcho-tang Suppresses Hepatic Lipid Accumulation and Inflammation in C57BL/6 Mice 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.17 - 26

최정원 (대구한의대학교 한의학과 본초학교실) , 조수정 ((재)경북지역사업평가단) , 신미래 (대구한의대학교 한의학과 본초학교실) , 박해진 (대구한의대학교 바이오융복합시험센터)

Abstract ▼ AI-Helper

Objective : The aim of the present study is to examine hepatic lipid-lowering and anti-inflammatory effects of silymarin combined with Jakyakgamcho-tang on non-alcoholic fatty liver disease in a high fat diet-induced obese mice model. Methods : C57BL/6 mice were divided into four dietary groups: (1) Normal, (2) Control (60% high-fat diet), (3) Control + silymarin 50 mg/kg/day (Silymarin), (4) Control + Silymarin 50 mg/kg/day + Jakyakgamcho-tang 100 mg/kg/day (SPG). After 12 weeks administration, mice were sacrificed and lipids and inflammation-related biomarkers were analyzed liver and plasma. Results : Silymarin and SPG treatments significantly lowered body and liver weights compared to the Control. Serumlipids (triglyceride (TG), total cholesterol) and pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 1𝛽, and IL-6) concentrations were significantly lowered in the Silymarin and SPG groups than the Control group. Silymarin and SPG treatments suppressed hepatic TG level and hepatic lipid droplets compared to the Control. Theses two treatments significantly increased hepatic kinase B1 and AMP-activated protein kinase protein levels, and significantly decreased hepatic key lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase and stearyl coenzyme A desaturase 1) protein levels than the Control. SPG also significantly increased hepatic fatty acid oxidation-related protein (peroxisome proliferator-activated receptor alpha and uncoupling protein 2) levels than the Control. Conclusions: Silymarin and SPG suppressed hepatic lipid accumulation by regulating hepatic protein expression, and lowered blood pro-inflammatory cytokines concentrations though the synergic effect of silymarin and Jakyakgamchotang was not clear.

주제어

표/그림 (8)

표 Table 1. Total Polyphenol Content and Antioxidant Property of Jakyakgamcho-tang
표 Table 2. Body Weight and Food Efficiency Ratio
그림 Fig. 1. Serum profiles. All values are mean±SE (n=9). ^##p < 0.01 and ^###p < 0.001, compared with Normal group. *p < 0.05 and ***p < 0.001, compared with Control group. Control, 60% high-fat diet group; Silymarin, Control with silymarin 50 mg/kg/day group; SPG, Silymarin with Jakyakgamcho-tang 100 mg/kg/day group. TC, total cholesterol, TG, triglyceride.
표 Table 3. Organ Weight (g/100 g body weight)
그림 Fig. 2. Hepatic lipids. All values are mean±SE (n=9). ^##p < 0.01 and ^###p < 0.001, compared with Normal group. ***p < 0.001, compared with Control group. Control, 60% high-fat diet group; Silymarin, Control with silymarin 50 mg/kg/day group; SPG, Silymarin with Jakyakgamchotang 100 mg/kg/day group.
그림 Fig. 3. Hepatic and white adipose tissue morphology. Control, 60% high-fat diet group; Silymarin, Control with silymarin 50 mg/kg/day group; SPG, Silymarin with Jakyakgamcho-tang 100 mg/kg/day group. H&E, Hematoxylin and eosin stain. ORO, oil red O stain.
그림 Fig. 4. Masson's Trichrome-stained liver sections. Control, 60% high-fat diet group; Silymarin, Control with silymarin 50 mg/kg/day group; SPG, Silymarin with Jakyakgamcho-tang 100 mg/kg/day group.
그림 Fig. 5. Hepatic protein expression. All values are mean±SE (n=9). ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001, compared with Normal group. *p < 0.05 and **p < 0.01, compared with Control group. Control, 60% high-fat diet group; Silymarin, Control with silymarin 50 mg/kg/day group; SPG, Silymarin with Jakyakgamcho-tang 100 mg/kg/day group.

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