[논문]부산 태종대 굴통머리 미역(Undaria pinnatifida)의 화학적 특성 및 항산화 효과

신영도; 이정우; 최명원; 임선영

doi:10.5657/kfas.2023.0196

부산 태종대 굴통머리 미역(Undaria pinnatifida)의 화학적 특성 및 항산화 효과
Chemical Characteristics and Antioxidant Effects of Sea Mustard Undaria pinnatifida from the Gultongmeori Area, Taejongdae, Busan 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.2, 2023년, pp.196 - 203

신영도 (한국해양대학교 해양과학융합학부) , 이정우 (한국해양대학교 해양과학융합학부) , 최명원 (한국해양대학교 해양과학융합학부) , 임선영 (한국해양대학교 해양과학융합학부)

Abstract ▼ AI-Helper

We investigated the nutritional characteristics and antioxidant effects of sea mustard Undaria pinnatifida fractions from Gultongmeori in Taejongdae, Youngdo, Busan. Based on dry weight, the moisture, crude protein, crude lipid, crude ash, and crude fiber contents were 34.98%, 11.55%, 0.43%, 17.82%, and 3.45%, respectively. To evaluate the antioxidant effect, we used radical scavenging (DPPH and ABTS), reactive oxygen species (ROS) production measurement, and DNA oxidation assays. Total flavonoid and phenol contents were higher in the n-hexane fraction than in other fractions. The n-hexane fraction was more effective at scavenging free radicals than other fractions as assessed using DPPH and ABTS assays (P<0.05). The ROS production assay showed that all sea mustard fractions decreased H2O2 induced cellular ROS production compared to that seen in the control (P<0.05); however, the n-hexane fraction reduced cellular ROS production to a greater extent than the other fractions. Furthermore, the n-hexane fraction from Gultongmeori significantly inhibited genomic DNA oxidation. These results indicate that the antioxidant effect of sea mustard is associated with its high flavonoid and phenol content. This study proposes that processed food products supplemented with sea mustard can be developed as functional foods to promote health in the local population.

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표/그림 (7)

그림 Fig. 1. Map of sea mustard Undaria pinnatifida collection sites in Taejongdai.
표 Table 1. Proximate composition of sea mustard Undaria pinnatifida of Gultongmeori areas in Taejoungdai, Yeongdo, Busan
$Table 2. Contents of Gultongmeori areas in Taejoungdai, Yeongdo, Busan of total flavonoids and phenols of fractions from sea mustard Undaria pinnatifida$ 표 Table 2. Contents of Gultongmeori areas in Taejoungdai, Yeongdo, Busan of total flavonoids and phenols of fractions from sea mustard Undaria pinnatifida
$Table 3. DPPH radical scavenging effect (%) of fractions from sea mustard Undaria pinnatifida$ 표 Table 3. DPPH radical scavenging effect (%) of fractions from sea mustard Undaria pinnatifida
$Table 4. ABTS radical scavenging effect (%) of fractions from sea mustard Undaria pinnatifida$ 표 Table 4. ABTS radical scavenging effect (%) of fractions from sea mustard Undaria pinnatifida
$Fig. 2. Inhibitory effect of solvent fractions from sea mustard Undaria pinnatifida from Gultongmeori areas in Taejongdai (A, 0.1 mg/mL; B, 0.05 mg/mL) on levels of reactive oxygen species in HT-1080 human fibrosarcoma cells. Control, Treated with H2O2 and distilled water; blank was treated with distilled water without H2O2; n-Hexane, n-Hexane fraction; 85% aq. MeOH, 85% aqueous methanol fraction; n- BuOH, n-butanol faction; Water, Water fraction. Values are expressed as mean±SD. Values with different letters are significantly different at P<0.01 using Tukey's test.$ 그림 Fig. 2. Inhibitory effect of solvent fractions from sea mustard Undaria pinnatifida from Gultongmeori areas in Taejongdai (A, 0.1 mg/mL; B, 0.05 mg/mL) on levels of reactive oxygen species in HT-1080 human fibrosarcoma cells. Control, Treated with H₂O₂ and distilled water; blank was treated with distilled water without H₂O₂; n-Hexane, n-Hexane fraction; 85% aq. MeOH, 85% aqueous methanol fraction; n- BuOH, n-butanol faction; Water, Water fraction. Values are expressed as mean±SD. Values with different letters are significantly different at P<0.01 using Tukey's test.
$Fig. 3. Antioxidant effects of solvent fractions from sea mustard Undaria pinnatifida on genomic DNA in HT-1080 cells. Control, Treated with H2O2 and distilled water; blank was treated with distilled water without H2O2. n-Hexane, n-Hexane fraction; 85% aq. MeOH, 85% aqueous methanol fraction; n-BuOH, n-butanol faction; Water, water fraction$ 그림 Fig. 3. Antioxidant effects of solvent fractions from sea mustard Undaria pinnatifida on genomic DNA in HT-1080 cells. Control, Treated with H₂O₂ and distilled water; blank was treated with distilled water without H₂O₂. n-Hexane, n-Hexane fraction; 85% aq. MeOH, 85% aqueous methanol fraction; n-BuOH, n-butanol faction; Water, water fraction

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대상 데이터

실험에 사용된 돌미역은 부산시 태종대 굴통머리 구역에서 2021년 3월에 채취되었다(Fig. 1).

데이터처리

실험결과는 Mean±SD (Standard deviation)으로 나타내었고 분석된 실험 데이터는 대조군과 각 시료로부터 얻은 실험 자료로부터 one-way ANOVA를 실시하여 P<0.05 수준에서 유의성을 검증하였고 사후검증은 Tukey’s test를 실시하였다(SAS program v.9.1, SAS Institute Inc., Cary, NC, USA).

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