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논문 상세정보

초록

총 아플라톡신 분석을 위한 최적의 추출 및 용출조건을 확립하여 최적의 전처리 조건을 확립하였다. 추출용매는 70% methanol 용액에 NaCl을 각각 1% 첨가한 용액을 사용하였고, 정제용 컬럼인 immunoaffinity column을 이용하여 acetonitrile 3 mL를 용출용매로 사용하여 아플라톡신 혼합용액의 분리능을 좋게 하였다. 검출한계는 0.05 ng/g 수준이였고, 식품의 유형별 총 아플라톡신($B_1,\;B_2,\;G_1,\;G_2$)의 회수율은 곡류 $74.1{\sim}95.5%,\;83.7{\sim}98.8%,\;80.4{\sim}102.4%$$72.8{\sim}76.5%$, 두류 $85.8~87.5%,\;83.8{\sim}90.7%,\;92.0{\sim}94.5%$$60.6{\sim}65.6%$, 견과류 $84.6{\sim}97.1%,\;86.0{\sim}94.1%,\;95.5{\sim}111.5%$$71.0{\sim}89.9%$, 가공식품 $81.5{\sim}87.1%,\;82.8{\sim}85.8%,\;85.4{\sim}92.7%$$68.9{\sim}76.4%$, 건조과실류 $83.6{\sim}93.5%,\;78.1{\sim}90.4%,\;93.0{\sim}108.5%$$64.9{\sim}78.5%$, 기타식품 $72.5{\sim}98.3%,\;73.1{\sim}96.4%,\;83.5{\sim}107.2%$$64.2{\sim}75.8%$를 보여주었다.

Abstract

We optimized conditions of extract solvents and elution solvents for total aflatoxins in foods using HPLC/FLD. The extract solvent was 70% methanol solution including 1% NaCl and the 3 mL of acetonitrile was used as elution solvent using immnuoaffinity column. The detection limits (LOD) was 0.05 ng/g. The recoveries for total aflatoxins ($B_1,\;B_2,\;G_1\;and\;G_2$) studied in foods were cereals ($74.1{\sim}95.5%,\;83.7{\sim}98.8%,\;80.4{\sim}102.4%,\;72.8{\sim}76.5%$), pulses ($85.8{\sim}87.5%,\;83.8{\sim}90.7%,\;92.0{\sim}94.5%,\;60.6{\sim}65.6%$), nuts ($84.6{\sim}97.1%,\;86.0{\sim}94.1%,\;95.5{\sim}111.5%,\;71.0{\sim}89.9%$), processed foods ($81.5{\sim}87.1%,\;82.8{\sim}85.8%,\;85.4{\sim}92.7%,\;68.9{\sim}76.4%$), dried fruits ($83.6{\sim}93.5%,\;78.1{\sim}90.4%,\;93.0{\sim}108.5%,\;64.9{\sim}78.5%$) and other foods ($72.5{\sim}98.3%,\;73.1{\sim}96.4%,\;83.5{\sim}107.2%,\;64.2{\sim}75.8%$), respectively.

참고문헌 (18)

  1. IARC, Monograph on the evaluation of carcinotenic risk to humans (Lyon:IARC), Vol. 82, pp. 171 (2002) 
  2. European Commission, Commission Regulation. available at [http://europa.eu.int/eur-lex/en/consleg/pdf/2001/en_ 2001R0466_do_001.pdf] 
  3. Food and Drug Administration, Action levels for poisonous or deleterious substances in human food and animal feed. available at [http://www.cfsan.fda.gov/ ~lrd/fdaact.html#afla] 
  4. Codex alimentarius, Maximum level and sampling plan for total aflatoxins in peanuts intended for further processing. available at [http://www.codexalimentarius.net/download/ standards/335/CXS_209e.pdf] 
  5. Korea Food and Drug Administration, Food Code, (2005) 
  6. Papp E., H-Otta K., Zaray G., Mincsovics E. Liquid chromatographic determination of aflatoxins, Microchemical J. 73, 39-46 (2002) 
  7. Boenke A. Method validation for mycotoxin determinations in food and feedstuffs, trends in analytical chemistry, 17(1), 10- 17 (1998) 
  8. Malone B. R., Humphrey C. W., Romer T. R., Richard J. L. Determination of aflatoxins in grains and raw peanuts by a rapid precedure with fluorometric analysis, Journal of AOAC International, 83(1), 95-98 (2000) 
  9. Asis R., Paola R. D., Aldao M. A. J. Determination of aflatoxin B1 in highly contaminated peanut samples using HPLC and ELISA, Food and Agricultural Immunology, 14, 201-208 (2002.) 
  10. Daradimos E, Marcaki P., Koupparis M. Evaluation and validation of two fluorometric HPLC methods for the determination of aflatoxin B1 in olive oil, Food Additives and Contaminants, 17(1), 65-73 (2000) 
  11. Akiyama H., Goda Y., Tanaka T., Toyoda M. Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up, Journal of Chromatography A, 932, 153-157 (2001) 
  12. Kim E. K., Shon D. H., Yoo J. Y., Ryu D., Lee C., Kim Y. B. Natural occurrence of aflatoxins in Korean Meju, Food Additives and Contaminants, 18(2), 151-156 (2001) 
  13. Stroka J., Anklam E., Jorissen U., Gilbert J. Immunoaffinity column cleanup with liquid chromatography using postcolumn bromination for determination of aflatoxins in peanut butter, pistachio paste, fig paste, and paprika powder: Collaborative study, Journal of AOAC International, 83(2), 320-340 (2000) 
  14. Mably M., Mankotia M., Cavlovic P., Tam J., Wong L., Pantazopoulos P., Calway P., Scott P. M. Survey of aflatoxins in beer sold in Canada, Food Additives and Contaminants, 22(12), 1252-1257 (2005) 
  15. AOAC Official Method 991.31, Afaltoxins in Corn, Raw Peanuts and Peanut butter, AOAC Official Methods of Analysis (1995) 
  16. Moller T. E., Nyberg M. Efficiency of different extraction solvent mixtures used in analyses of aflatoxins from a certified peanut meal reference material, Food Additives and Contaminants, 21(8), 781-785 (2004) 
  17. Chan D., MacDonald S. J., Boughtflower V., Brereton P. Simultaneous determination of aflatoxins and ochratoxin A in food using a fully automated immunoaffinity column clean-up and liquid chromatography-fluorescence detection, Journal of Chromatography A, 1059, 13-16 (2004) 
  18. Pearson S. M., Candlish A. A. G., Aidoo K. E., Smith J. E. Determination of aflatoxin levels in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescence detection, Biotechnology Techniques, 13, 97-99 (1999) 

이 논문을 인용한 문헌 (4)

  1. Jang, Mi-Ran ; Lee, Chang-Hee ; Cho, Sung-Hye ; Park, Joon-Shik ; Kwon, Eun-Young ; Lee, Eun-Jin ; Kim, So-Hee ; Kim, Dai-Byung 2007. "A Survey of Total Aflatoxins in Food Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Liquid Chromatography Tandem Mass Spectrometry(LC-MS/MS)" 한국식품과학회지 = Korean journal of food science and technology, 39(5): 488~493 
  2. Song, Hyuk_hwan ; Lee, Hee-Seok ; Lee, Chan 2008. "Survey of Beauvericin Contamination in Korean Grains by HPLC and the Production of Beauvericin and Enniatin Derivatives by Fusarium oxysporum KFCC 11363P" 한국식품위생안전성학회지 = Journal of food hygiene and safety, 23(1): 73~79 
  3. Kim, Kyeong-Yeol ; Nam, Min-Ji ; Nam, Bo-Ram ; Ryu, Hee-Jung ; Song, Jeong-Eon ; Shim, Won-Bo ; Lee, Soo-Hyung ; Chung, Duck-Hwa 2010. "Determination of Total Aflatoxins in Foods by Parallelism of ELISA and LC/MS/MS" 韓國環境保健學會誌 = Journal of environmental health sciences, 36(1): 52~60 
  4. Park, Ji-Won ; Yoo, Myung-Sang ; Kuk, Ju-Hee ; Ji, Young-Ae ; Lee, Jin-Ha 2013. "Simultaneous Determination and Mornitoring of Aflatoxin and Ochratoxin A in Food" 한국식품위생안전성학회지 = Journal of food hygiene and safety, 28(1): 75~82 

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