[논문]말에서 개체식별 및 친자확인을 위한 ISAG Microsatellite Marker의 유용성 및 표준화

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[국내논문] 말에서 개체식별 및 친자확인을 위한 ISAG Microsatellite Marker의 유용성 및 표준화
Standardization and Usefulness of ISAG Microsatellite Markers for Individual Identification and Parentage Verification in Horse Breeds 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.26 no.3 = no.68, 2009년, pp.220 - 225

초록
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말에서 개체식별 및 친자확인을 위한 ISAG microsatellite marker의 적용을 위한 유용성 및 표준화를 위해 더러브렛종 6두를 포함한 다양한 품종의 20두를 대상으로 22개의 ISAG microsatellite marker를 분석한 결과 대립유전자의 수는 4개에서 8개로 분포하였고 더러브렛종에서 출현하는 대립유전자의 대부분이 비더러브렛종에서도 출현하였다. 본 연구결과를 통해서 ISAG microsatellte marker를 제주말의 혈통등록에 적용할 수 있음을 알 수 있었다.

Abstract ▼ AI-Helper

The present study demonstrates a new approach that enables effective horse parentage testing using 22 ISAG microsatellite markers involving 6 heads of Thoroughbred horse(TB) and non-TB. In the comparison allele distribution between these horses, the alleles found in the TB were numerously detected in the non-TB. As results, we confirmed that these ISAG microsatellite markers might apply the pedigree registration of Korean native horse(Jeju horse).

주제어

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문제 정의

The aim of the comparison test of ISAG is to enable laboratories working on immunogenetics and to maintain high standards in DNA and biochemical polymorphisms of animals. Furthermore, it is valuable to entered into international agreement on nomenclature and typing procedures.
However, the microsatellite markers are differented with ISAG markers in Jeju horse. This study was conducted to standardize the application of ISAG horse markers for parentage verification and individual identification in horse breeds, especially Jeju horse.

제안 방법

The Committee has recommended that parentage testing should consist of an exclusion based on the incompatibility of two or more markers, because an exclusion based on a single marker may involve an element of uncertainty. All possibilities should be tried to obtain additional information to support a decision for such an exclusion, including test for additional markers or mutation analysis (1).
This suggests that the judgment criterion for registration by evaluating allele distribution needs to be revised, especially for basic registration in the Jeju horse. Also, after genotyping, parentage testing was performed according to Mendelian fashion and ISAG guideline. As demonstrated in this study, 2 foals were not inherited alleles from sire or dam, and excluded by the incompatibility of 13 and 17 markers, respectively.

대상 데이터

The results of parentage testing in 2 foals (TB foal, TB and Irish Draught Cross breed, respectively) are shown in Table 3. 2 foals were not inherited alleles from sire or dam, and excluded by the incompatibility of 13 (AHT5, ASB2, ASB23, HMS1, HMS2, HMS3, HTG10, LEX3, TKY279, TKY287, TKY297 and VHL20 in TB) and 17 (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS7, HTG4, HTG10, LEX3, LEX33, TKY279, TKY287 and TKY297) markers, respectively.
Also, after genotyping, parentage testing was performed according to Mendelian fashion and ISAG guideline. As demonstrated in this study, 2 foals were not inherited alleles from sire or dam, and excluded by the incompatibility of 13 and 17 markers, respectively. From our data, we confirmed that these ISAG microsatellite markers might apply the pedigree registration of Korean native horse and useful tools for managing to breed Jeju horse.
Genomic DNAs from 20 horse blood samples (1 Cob horse, 1 Connemara pony, 8 Irish Draught horse, 6 TB, 3 Thoroughbred and Irish Draught Cross horse, 1 Tobiano horse) for 2007/2008 ISAG horse comparison test were extracted by QIAamp DNA Blood Maxi Kit according to the manufacturer's protocols. Twenty-two ISAG microsatellite markers were selected for this study (Table 1). These microsatellite markers have been reported by the Horse Applied Genetics Committee of ISAG for individual identification and parentage verification of horse breeds.

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