[논문]표고버섯 수확후배지 퇴비 추출물에서 Bacillus velezensis HKB-1의 배양적 특징 및 고추역병의 생물학적 방제

김자윤; 서현지; 강대선; 강희완

doi:10.14480/jm.2021.19.4.272

[국내논문] 표고버섯 수확후배지 퇴비 추출물에서 Bacillus velezensis HKB-1의 배양적 특징 및 고추역병의 생물학적 방제
Cultural characteristics of Bacillus velezensis HKB-1 in the water extract of the composted spent mushroom substrate of Lentinula edodes and biological control of Phytophthora blight disease of pepper 원문보기

Journal of mushrooms = 한국버섯학회지, v.19 no.4, 2021년, pp.272 - 278

김자윤 (한경대학교 생명공학부 원예생명공학전공) , 서현지 (한경대학교 생명공학부 원예생명공학전공) , 강대선 ((주)케이글로벌) , 강희완 (한경대학교 생명공학부 원예생명공학전공)

초록
AI-Helper

Bacillus velezensis HKB-1가 표고버섯 수확후배지 퇴비로부터 분리되었으며 고추역병균(Phythopthora capsici), 인삼모잘록병균(Rhizoctonia solani), 고추탄저병균(Collectotrichum coccodes) 및 시들음병(Fusarium oxysporium)의 균사체 성장을 70% 이상 억제하는 항 진균 활성을 보였다. B. velezensis HKB-1은 표고버섯 수확후배지 퇴비 물 추출물과 당밀 1% 첨가배지에서 다른 상업용 세균배지보다 10~100배 더 높은 세균증식률을 보였으며 고추 역병균의 균사체 생장을 90% 억제하였으며 고추생육 촉진효과 및 고추역병에 대하여 70% 이상의 방제효과가 있었다.

Abstract ▼ AI-Helper

Bacillus velezensis HKB-1 was isolated from the composted spent mushroom substrate of Lentinula edodes (LeCSMS) and inhibited mycelial growth of phytopathogenic fungal species, Phythhopthora capsici, Collectotrichum coccodes and Fusarium oxysporium by more than 70%. B. velezensis HKB-1 showed bacterial growth rate 10 to 100 times higher than that of other commercial bacterial media in water extract of LeCSMS supplemented with 1% molasses. The LeCSMS medium was effective in promoting the growth of pepper and controlling Phytophthora blight disease of pepper.

Keyword

표/그림 (8)

그림 Fig. 1. Antifungal activity of Bacillus velezenesis HKB-1 cells against mycelia of different phytopathogenic fungal species.
표 Table 1. Physicochemical characteristics in water extract from composted spent mushroom substrate of Lentinula edodes cultured with Bacillus velezensis HKB-1
표 Table 2. Metal ion content in water extract from composted spent mushroom substrate of Lentinula edodes cultured with Bacillus velezensis HKB-1
표 Table 3. The cell density of Bacillus velezensis HKB-1 cultured in different media
그림 Fig. 2. Mycelial growth inhibition of Phythopthora capsici on different culture media of Bacillus velezensis HKB-1.
표 Table 4. Growth promotion of pepper seedlings by LeCSMS water extract cultured with Bacillus velezensis HKB-1
표 Table 5. Mycelial growth inhibition of Phythopthora capsici on different culture media of Bacillus velezensis HKB-1
그림 Fig. 3. Protective effect of Bacillus velezensis HKB-1 cultured in water extract from composted spent mushroom substrate (WESMS) of Lentinula edodes against Phytophthora blight disease of pepper. The diseases index was checked on pepper for days after inoculation (A) and disease severity was observed after 8 days of inoculation (B). Value represents the mean disease index ± standard deviations of three replicates for each treatment.

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