[논문]비스페놀A 유도 간 손상 마우스에서 해삼(Stichopus japonicas) 추출물 및 가수분해물의 간 기능 개선 효과

김세정; 조윤호; 박비오; 유대석; 김두호; 김민정; 곽연길; 김진성

doi:10.15433/ksmb.2022.14.2.061

비스페놀A 유도 간 손상 마우스에서 해삼(Stichopus japonicas) 추출물 및 가수분해물의 간 기능 개선 효과
Extracts and Enzymatic Hydrolysates Derived from Sea Cucumber Stichopus japonicas Ameliorate Hepatic Injury in BisphenolA-treated Mice 원문보기

한국해양바이오학회지 = Journal of marine bioscience and biotechnology, v.14 no.2, 2022년, pp.61 - 68

김세정 (금산인삼약초산업진흥원) , 조윤호 (금산인삼약초산업진흥원) , 박비오 (금산인삼약초산업진흥원) , 유대석 (금산인삼약초산업진흥원) , 김두호 (휴온스푸디언스 기업부설연구소) , 김민정 (휴온스푸디언스 기업부설연구소) , 곽연길 (휴온스푸디언스 기업부설연구소) , 김진성 (금산인삼약초산업진흥원)

Abstract ▼ AI-Helper

This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.

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표/그림 (6)

표 Table 1. List of samples (extracts and hydrolysates) derived from Stichopus japonicas used in this study
그림 Fig. 1. Cytotoxicity (a), NO production (b), and ROS producti on (c) in RAW 264.7 treated with sea cucumber extrac ts (3,000 μg/mL). *: means significantly decreased co mpared to control or LPS (p < 0.05*, 0.01**, 0.001** *). Con; control, LPS; treated with lipopolysaccharide 100 ng/mL.
그림 Fig. 2. Cytotoxicity (a), NO production (b), and ROS production (c) in RAW 264.7 treated with sea cucumber hydrolysates. *: means significantly decreased compared to control or LPS (p < 0.05*, 0.01**, 0.001***). Con; control, LPS; treated with lipopolysaccharide 100 ng/mL, pep-L; 3,000 and 5,000 μg/mL, PNL-L; 1,000 and 2,000 μg/mL.
그림 Fig. 3. Cytotoxicity (a), lipid production (b), and cell recovery (c) in HepG2 treated with sea cucumber extracts (3,000 μg/mL). *: means significantly decreased, #: means significantly increased compared to control, PA, or BPA (p < 0.05*, 0.01**, 0.001***). Con; control, PA; treated with palmitic acid 300 μM, BPA; treated with bisphenolA 200 μM.
그림 Fig. 4. Serum ALT (a) and AST (b) level in ICR mouse treated with bisphenolA and sea cucumber samples. *: means significantly decreased compared to control or LPS (p < 0.05*, 0.01**, 0.001***). Con; saline solution, B-V/B-; bisphenolA 20 mg/kg, SM; silymarin 80 mg/kg, 85-Dw1; 1 g/kg, 85-Dw2; 2 g/kg, Pep-L; 5 g/kg.
그림 Fig. 5. The representative histological profiles of the liver sections. The liver sections were stained with hematoxylin and eosin (H&E). X100. (a) saline solution, (b) bisphenol A 20 mg/kg, (c) silymarin 80 mg/kg, (d) 85-Dw1 1 g/kg, (e) 85-Dw2 2 g/kg, (f) Pep-L 5 g/kg.

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