[논문]Sub-lethal toxicity assessment of the phenylurea herbicide linuron in developing zebrafish (Danio rerio) embryo/larvae

Maharaj, Sapna; El Ahmadie, Nader; Rheingold, Spencer; El Chehouri, Jana; Yang, Lihua; Souders II, Christopher L.; Martyniuk II, Christopher J.

doi:10.1016/j.ntt.2020.106917

Sub-lethal toxicity assessment of the phenylurea herbicide linuron in developing zebrafish (Danio rerio) embryo/larvae

Neurotoxicology and teratology, v.81, 2020년, pp.106917 -

Maharaj, Sapna (Department of Physiological Sciences, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida) , El Ahmadie, Nader (Department of Physiological Sciences, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida) , Rheingold, Spencer (Department of Physiological Sciences, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida) , El Chehouri, Jana (Department of Physiological Sciences, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida) , Yang, Lihua (Department of Physiological Sciences, University of Florida Genetics Institute, Interdisciplinary Program in Bio) , Souders II, Christopher L. , Martyniuk II, Christopher J.

Abstract ▼ AI-Helper

Abstract Due to run-off and rain events, agrochemicals can enter water catchments, exerting endocrine disruption effects and toxicity to aquatic organisms. Linuron is a phenylurea herbicide used to control a wide variety of vegetative weeds in agriculture in addition to residential applications. However, there are few studies that quantify its toxicity to early developmental stages of fish. The objectives of this study were to assess the acute toxicity of linuron to zebrafish embryos/larvae by measuring mortality, morphological deformities, oxidative respiration, gene expression, and locomotor activity via the Visual Motor Response test. Zebrafish embryos at ~6-h post-fertilization (hpf) were exposed to either embryo rearing medium (ERM), or one dose of 0.625, 1.25, 2.5, 5, and 10 μM linuron for up to 7 days post-fertilization (dpf) depending on the assay. Zebrafish larvae exposed to linuron displayed pericardial edema, yolk sac edema, and spinal curvature. Oxidative respiration assessments in embryos using the Agilent XFe24 Flux Analyzer revealed that linuron decreased mean basal respiration and oligomycin-induced ATP-linked respiration in 30 hpf embryos at 20 μM after a 24-hour exposure. In 7 dpf larvae, transcript abundance was determined for 6 transcripts that have a role in oxidative respiration (atp06, cox1, cox4-1, cox5a1, cytb, and nd1); the relative abundance of these transcripts was not altered with linuron treatment. A Visual Motor Response test was conducted on 7 dpf larvae to determine whether linuron (0.625 to 5 μM) impaired locomotor activity. Larval activity in the dark period decreased in a dose dependent manner and there were indications of hypoactivity as low as 1.25 μM. Transcript abundance was thus determined for tyrosine hydroxylase (th1) and glutamic acid decarboxylase 67 (gad1b), two rate limiting enzymes that control the production of dopamine and gamma-aminobutyric acid respectively. The mRNA levels of gad1b (p = 0.019) were reduced with increasing concentrations of linuron while th1 (p = 0.056) showed a similar decreasing trend, suggesting that neurotransmitter biosynthesis may be altered with exposure to linuron. This study improves knowledge related to the toxicity mechanisms for linuron and is the first to demonstrate that this anti-androgenic chemical impairs oxidative respiration and exerts neurotoxic effects associated with neurotransmitter biosynthesis during early development. These data are significant for environmental risk assessment of agrochemicals. Highlights Linuron induced significant mortality and deformity at 5 to 10 μM in larval zebrafish. Oxygen consumption rates in zebrafish embryos decreased with increasing exposure concentrations. Linuron at concentrations as low as 1.25 μM induced hypoactivity in larval zebrafish. Transcript levels of glutamic acid decarboxylase 1B were reduced with increasing concentrations of linuron.

주제어

참고문헌 (45)

Zebrafish Bortolotto 11 2 142 2014 10.1089/zeb.2013.0923 Long-term exposure to paraquat alters behavioral parameters and dopamine levels in adult zebrafish (Danio rerio)

상세보기
Aquat. Toxicol. Cao 198 129 2018 10.1016/j.aquatox.2018.02.023 Short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish (Danio rerio)

상세보기
Chemosphere Cao 214 303 2019 10.1016/j.chemosphere.2018.09.105 Developmental toxicity of the fungicide ziram in zebrafish (Danio rerio)

상세보기
Environ. Sci. Pollut. Res. Int. Cao 26 5 4913 2019 10.1007/s11356-018-3957-z Developmental toxicity of the triazole fungicide cyproconazole in embryo-larval stages of zebrafish (Danio rerio)

상세보기
Environmental Toxicology and Water Quality: An International Journal Caux 13 1 1 1998 10.1002/(SICI)1098-2256(1998)13:1<1::AID-TOX1>3.0.CO;2-B Canadian water quality guidelines for linuron

상세보기
Neuroscience Cocco 343 300 2017 10.1016/j.neuroscience.2016.07.018 Characterization of the γ-aminobutyric acid signaling system in the zebrafish (Danio rerio Hamilton) central nervous system by reverse transcription-quantitative polymerase chain reaction

상세보기
Aquat. Toxicol. Crago 110-111 74 2012 10.1016/j.aquatox.2011.12.021 A mixture of an environmentally realistic concentration of a phthalate and herbicide reduces testosterone in male fathead minnow (Pimephales promelas) through a novel mechanism of action

상세보기
Chemosphere Crumrkz 36 10 2175 1998 10.1016/S0045-6535(97)10190-4 Fate of the herbicide linuron in outdoor experimental ditches

상세보기
Arch. Environ. Contam. Toxicol. da Costa Chaulet 77 3 443 2019 10.1007/s00244-019-00644-7 Glyphosate- and fipronil-based agrochemicals and their mixtures change zebrafish behavior

상세보기
Environ. Toxicol. Pharmacol. da Silva Simoes 54 53 2017 10.1016/j.etap.2017.06.024 The metabolic effects of diuron in the rat liver

상세보기
Environ. Toxicol. Chem. Dai 33 1 11 2014 10.1002/etc.2406 Zebrafish as a model system to study toxicology

상세보기
J. Exp. Biol. Duggan 214 Pt 11 1880 2011 10.1242/jeb.053322 Coordination of cytochrome c oxidase gene expression in the remodelling of skeletal muscle

상세보기
Toxicol. Lett. Fraser 276 62 2017 10.1016/j.toxlet.2017.05.021 Toxicant induced behavioural aberrations in larval zebrafish are dependent on minor methodological alterations

상세보기
Aquat. Toxicol. Fraser 193 128 2017 10.1016/j.aquatox.2017.10.002 Endocrine disruptors affect larval zebrafish behavior: testing potential mechanisms and comparisons of behavioral sensitivity to alternative biomarkers

상세보기
J. Environ. Sci. Fu 63 116 2018 10.1016/j.jes.2017.09.010 Multiresidue determination and potential risks of emerging pesticides in aquatic products from Northeast China by LC-MS/MS

상세보기
Cell Death Dis. Gatliff 8 6 e2896 2017 10.1038/cddis.2017.186 A role for TSPO in mitochondrial Ca 2+ homeostasis and redox stress signaling

상세보기
Pakistan Journal of Weed Science Research Hayyat 22 3 2016 Screening of herbicides for efficient control of broadleaf weeds in wheat (Triticum aestivum L.)
Environ. Toxicol. Chem. Hogan 31 6 1391 2012 10.1002/etc.1815 Exposure to the pesticide linuron affects androgen-dependent gene expression in the three-spined stickleback (Gasterosteus aculeatus)

상세보기
Environ. Sci. Eur. Legradi 30 1 46 2018 10.1186/s12302-018-0173-x An ecotoxicological view on neurotoxicity assessment

상세보기
Environ. Pollut. Liang 245 272 2019 10.1016/j.envpol.2018.10.130 Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio)

상세보기
Comp Biochem Physiol C Toxicol Pharmacol Marlatt 194 9 2017 10.1016/j.cbpc.2017.01.002 Biological responses to phenylurea herbicides in fish and amphibians: new directions for characterizing mechanisms of toxicity

상세보기
Comp Biochem Physiol C Toxicol Pharmacol Marlatt 157 1 24 2013 10.1016/j.cbpc.2012.09.001 The effects of the urea-based herbicide linuron on reproductive endpoints in the fathead minnow (Pimephales promelas)

상세보기
J. Appl. Bacteriol. Maule 57 2 369 1984 10.1111/j.1365-2672.1984.tb01403.x Herbicide effects on the population growth of some green algae and cyanobacteria

상세보기
McGee 2004 Survey of Pesticide Use in Ontario, 2003
Comp Biochem Physiol Part D Genomics Proteomics Ornostay 8 4 263 2013 10.1016/j.cbd.2013.08.001 Classifying chemical mode of action using gene networks and machine learning: a case study with the herbicide linuron

상세보기
Patterson 2004 Analysis of Risks to Endangered and Threatened Salmon and Steelhead
Nucleic Acids Res. Pfaffl 29 9 e45 2001 10.1093/nar/29.9.e45 A new mathematical model for relative quantification in real-time RT-PCR

상세보기
Aquat. Toxicol. Pottinger 140-141 145 2013 10.1016/j.aquatox.2013.05.020 Anti-androgens act jointly in suppressing spiggin concentrations in androgen-primed female three-spined sticklebacks - prediction of combined effects by concentration addition

상세보기
Sci. Total Environ. Quintaneiro 586 390 2017 10.1016/j.scitotenv.2016.11.153 Endocrine and physiological effects of linuron and S-metolachlor in zebrafish developing embryos

상세보기
Toxicol. in Vitro Santos 28 5 932 2014 10.1016/j.tiv.2014.04.004 Toxicity of the herbicide linuron as assessed by bacterial and mitochondrial model systems

상세보기
Reproductive toxicology (Elmsford, N.Y.) Schiller 42 210 2013 10.1016/j.reprotox.2013.09.003 Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption

상세보기
Aquat. Toxicol. Schiller 155 62 2014 10.1016/j.aquatox.2014.06.005 Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption

상세보기
Genes Brain Behav. Shontz 17 4 e12460 2018 10.1111/gbb.12460 Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

상세보기
Aquat. Ecol. Snel 32 2 113 1998 10.1023/A:1009971930626 Inhibition of photosystem II (PSII) electron transport as a convenient endpoint to assess stress of the herbicide linuron on freshwater plants
Ecotoxicol. Environ. Saf. Souza 168 479 2019 10.1016/j.ecoenv.2018.10.070 Difenoconazole and linuron dissipation kinetics in carrots under open-field conditions

상세보기
Bull. Environ. Contam. Toxicol. Struger 87 4 420 2011 10.1007/s00128-011-0361-5 Occurrence and distribution of sulfonylurea and related herbicides in central Canadian surface waters 2006-2008

상세보기
Environ. Toxicol. Pharmacol. Topal 49 14 2017 10.1016/j.etap.2016.11.009 Evaluation of 8-hydroxy-2-deoxyguanosine and NFkB activation, oxidative stress response, acetylcholinesterase activity, and histopathological changes in rainbow trout brain exposed to linuron

상세보기
Environ. Sci. Technol. Uren Webster 49 5 3110 2015 10.1021/es505498u The herbicide linuron inhibits cholesterol biosynthesis and induces cellular stress responses in brown trout

상세보기
United States Environmental Protection Agency, Reregistration Eligibility Decision (RED) for Linuron March 1995, Washington, D.C. 20460.
United States Environmental Protection Agency, Reregistration Eligibility Decision (RED) for Diuron September 30, 2003, Washington, D.C. 20460.
U.S. Environmental Protection Agency (U.S. EPA) 9 1984 Chemical Information Fact Sheet Number 28: Linuron
Toxicol. Lett. Wang 283 13 2018 10.1016/j.toxlet.2017.10.022 Mitochondrial bioenergetics and locomotor activity are altered in zebrafish (Danio rerio) after exposure to the bipyridylium herbicide diquat

상세보기
Chemosphere Wang 191 106 2018 10.1016/j.chemosphere.2017.10.032 Paraquat affects mitochondrial bioenergetics, dopamine system expression, and locomotor activity in zebrafish (Danio rerio)

상세보기
Westerfield 1995 The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Brachydanio rerio)
J. Biochem. Mol. Toxicol. Zhao 20 3 103 2006 10.1002/jbt.20126 Interaction of diuron and related substituted phenylureas with the Ah receptor pathway

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Sub-lethal toxicity assessment of the phenylurea herbicide linuron in developing zebrafish (Danio rerio) embryo/larvae

Abstract ▼ AI-Helper

주제어

참고문헌 (45)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트